Glomerular Epithelial and Mesangial Cell Culture and Characterization

Heather M Wilson; Keith N Stewart

doi:10.1007/978-1-61779-367-7_13

Glomerular Epithelial and Mesangial Cell Culture and Characterization

Heather M Wilson, Keith N Stewart

Applied Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Culture of isolated kidney glomerular cells has been employed for almost four decades as a tool to dissect pathophysiological effects of individual cell types in renal disease. This chapter aims to highlight in detail the available techniques to isolate, culture, and characterize human glomerular epithelial and mesangial cells. To establish primary culture of these cells, glomeruli are isolated from the cortex of kidney by differential sieving and cellular outgrowths from cultured glomeruli further subcultured in appropriately coated tissue culture plates/flasks. Methods used for characterization of isolated glomerular mesangial and epithelial cells (podocytes) are described as are the phenotypic markers useful for identification. Other sources of isolated glomerular cells such as immortalized cell lines are briefly discussed.

Original language	English
Pages (from-to)	187-201
Number of pages	15
Journal	Methods in Molecular Biology
Volume	806
Early online date	7 Oct 2011
DOIs	https://doi.org/10.1007/978-1-61779-367-7_13
Publication status	Published - 2012

Bibliographical note

Wilson H.M., Stewart K.N. (2012) Glomerular Epithelial and Mesangial Cell Culture and Characterization. In: Mitry R., Hughes R. (eds) Human Cell Culture Protocols. Methods in Molecular Biology (Methods and Protocols), vol 806. Humana Press. Print ISBN 978-1-61779-366-0 Online ISBN 978-1-61779-367-7

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doi = "10.1007/978-1-61779-367-7_13",

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AB - Culture of isolated kidney glomerular cells has been employed for almost four decades as a tool to dissect pathophysiological effects of individual cell types in renal disease. This chapter aims to highlight in detail the available techniques to isolate, culture, and characterize human glomerular epithelial and mesangial cells. To establish primary culture of these cells, glomeruli are isolated from the cortex of kidney by differential sieving and cellular outgrowths from cultured glomeruli further subcultured in appropriately coated tissue culture plates/flasks. Methods used for characterization of isolated glomerular mesangial and epithelial cells (podocytes) are described as are the phenotypic markers useful for identification. Other sources of isolated glomerular cells such as immortalized cell lines are briefly discussed.

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

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Glomerular Epithelial and Mesangial Cell Culture and Characterization

Abstract

Bibliographical note

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