Heat-Stress Responses Differ among Species from Different ‘Bangia’ Clades of Bangiales (Rhodophyta)

Ho Viet Khoa; Puja Kumari; Hiroko Uchida; Akio Murakami; Satoshi Shimada; Koji Mikami

doi:10.3390/plants10081733

Heat-Stress Responses Differ among Species from Different ‘Bangia’ Clades of Bangiales (Rhodophyta)

Ho Viet Khoa, Puja Kumari, Hiroko Uchida, Akio Murakami, Satoshi Shimada, Koji Mikami

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

3 Downloads (Pure)

Abstract

The red alga ‘Bangia’ sp. ESS1, a ‘Bangia’ 2 clade member, responds to heat stress via accelerated asexual reproduction and acquires thermotolerance based on heat-stress memory. However, whether these strategies are specific to ‘Bangia’ 2, especially ‘Bangia’ sp. ESS1, or whether they are employed by all ‘Bangia’ species is currently unknown. Here, we examined the heat-stress responses of ‘Bangia’ sp. ESS2, a newly identified ‘Bangia’ clade 3 member, and Bangia atropurpurea. Intrinsic thermotolerance differed among species: Whereas ‘Bangia’ sp. ESS1 survived at 30 °C for 7 days, ‘Bangia’ sp. ESS2 and B. atropurpurea did not, with B. atropurpurea showing the highest heat sensitivity. Under sublethal heat stress, the release of asexual spores was highly repressed in ‘Bangia’ sp. ESS2 and completely repressed in B. atropurpurea, whereas it was enhanced in ‘Bangia’ sp. ESS1. ‘Bangia’ sp. ESS2 failed to acquire heat-stress tolerance under sublethal heat-stress conditions, whereas the acquisition of heat tolerance by priming with sublethal high temperatures was observed in both B. atropurpurea and ‘Bangia’ sp. ESS1. Finally, unlike ‘Bangia’ sp. ESS1, neither ‘Bangia’ sp. ESS2 nor B. atropurpurea acquired heat-stress memory. These findings provide insights into the diverse heat-stress response strategies among species from different clades of ‘Bangia’.

Original language	English
Article number	1733
Number of pages	15
Journal	Plants
Volume	10
Issue number	8
Early online date	22 Aug 2021
DOIs	https://doi.org/10.3390/plants10081733
Publication status	Published - 22 Aug 2021

Bibliographical note

Acknowledgments: We are grateful to Hiromi Nakao from Betsukai Fishery Cooperative, Shyoichi Itakura from the Hiyama Fisheries Extension Office, Yoshitomo Hamaya from the Esashi Branch of the Fisheries Co-operative Association of Hiyama, and Hidehiko Sasaki from the Main Branch of the
Fisheries Co-operative Association of Hiyama for permission and support in collecting seaweed on Kamomejima Island in Esasi. We also thank Ryunosuke Irie for technical assistance in phylogenetic analysis, Chengze Li for the maintenance of ‘Bangia’ sp. ESS2, and Yuji Hiwatashi for technical assistance in microscopic observation. Ho Viet Khoa was supported by Ministry of Education, Culture, Sports, Science and Technology of Japan. Puja Kumari is an International Research Fellow supported by JSPS with Postdoctoral Fellowship for Research in Japan (Standard). We also thank Hiroyuki Mizuta for permission of Ho Viet Khoa to study in the Miyagi University

Keywords

Bangia atropurpurea
‘Bangia’ sp
heat stress
asexual reproduction
stress memory
thermotolerance
‘Bangia’ sp.

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Khoa_etal_MDPI_Heat_Stress_Responses_VoR
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Cite this

@article{d144d1bb658944309418393302582ee3,

title = "Heat-Stress Responses Differ among Species from Different {\textquoteleft}Bangia{\textquoteright} Clades of Bangiales (Rhodophyta)",

abstract = "The red alga {\textquoteleft}Bangia{\textquoteright} sp. ESS1, a {\textquoteleft}Bangia{\textquoteright} 2 clade member, responds to heat stress via accelerated asexual reproduction and acquires thermotolerance based on heat-stress memory. However, whether these strategies are specific to {\textquoteleft}Bangia{\textquoteright} 2, especially {\textquoteleft}Bangia{\textquoteright} sp. ESS1, or whether they are employed by all {\textquoteleft}Bangia{\textquoteright} species is currently unknown. Here, we examined the heat-stress responses of {\textquoteleft}Bangia{\textquoteright} sp. ESS2, a newly identified {\textquoteleft}Bangia{\textquoteright} clade 3 member, and Bangia atropurpurea. Intrinsic thermotolerance differed among species: Whereas {\textquoteleft}Bangia{\textquoteright} sp. ESS1 survived at 30 °C for 7 days, {\textquoteleft}Bangia{\textquoteright} sp. ESS2 and B. atropurpurea did not, with B. atropurpurea showing the highest heat sensitivity. Under sublethal heat stress, the release of asexual spores was highly repressed in {\textquoteleft}Bangia{\textquoteright} sp. ESS2 and completely repressed in B. atropurpurea, whereas it was enhanced in {\textquoteleft}Bangia{\textquoteright} sp. ESS1. {\textquoteleft}Bangia{\textquoteright} sp. ESS2 failed to acquire heat-stress tolerance under sublethal heat-stress conditions, whereas the acquisition of heat tolerance by priming with sublethal high temperatures was observed in both B. atropurpurea and {\textquoteleft}Bangia{\textquoteright} sp. ESS1. Finally, unlike {\textquoteleft}Bangia{\textquoteright} sp. ESS1, neither {\textquoteleft}Bangia{\textquoteright} sp. ESS2 nor B. atropurpurea acquired heat-stress memory. These findings provide insights into the diverse heat-stress response strategies among species from different clades of {\textquoteleft}Bangia{\textquoteright}.",

keywords = "Bangia atropurpurea, {\textquoteleft}Bangia{\textquoteright} sp, heat stress, asexual reproduction, stress memory, thermotolerance, {\textquoteleft}Bangia{\textquoteright} sp.",

author = "Khoa, {Ho Viet} and Puja Kumari and Hiroko Uchida and Akio Murakami and Satoshi Shimada and Koji Mikami",

note = "Acknowledgments: We are grateful to Hiromi Nakao from Betsukai Fishery Cooperative, Shyoichi Itakura from the Hiyama Fisheries Extension Office, Yoshitomo Hamaya from the Esashi Branch of the Fisheries Co-operative Association of Hiyama, and Hidehiko Sasaki from the Main Branch of the Fisheries Co-operative Association of Hiyama for permission and support in collecting seaweed on Kamomejima Island in Esasi. We also thank Ryunosuke Irie for technical assistance in phylogenetic analysis, Chengze Li for the maintenance of {\textquoteleft}Bangia{\textquoteright} sp. ESS2, and Yuji Hiwatashi for technical assistance in microscopic observation. Ho Viet Khoa was supported by Ministry of Education, Culture, Sports, Science and Technology of Japan. Puja Kumari is an International Research Fellow supported by JSPS with Postdoctoral Fellowship for Research in Japan (Standard). We also thank Hiroyuki Mizuta for permission of Ho Viet Khoa to study in the Miyagi University",

year = "2021",

month = aug,

day = "22",

doi = "10.3390/plants10081733",

language = "English",

volume = "10",

journal = "Plants",

issn = "2223-7747",

publisher = "MDPI",

number = "8",

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TY - JOUR

T1 - Heat-Stress Responses Differ among Species from Different ‘Bangia’ Clades of Bangiales (Rhodophyta)

AU - Khoa, Ho Viet

AU - Kumari, Puja

AU - Uchida, Hiroko

AU - Murakami, Akio

AU - Shimada, Satoshi

AU - Mikami, Koji

N1 - Acknowledgments: We are grateful to Hiromi Nakao from Betsukai Fishery Cooperative, Shyoichi Itakura from the Hiyama Fisheries Extension Office, Yoshitomo Hamaya from the Esashi Branch of the Fisheries Co-operative Association of Hiyama, and Hidehiko Sasaki from the Main Branch of the Fisheries Co-operative Association of Hiyama for permission and support in collecting seaweed on Kamomejima Island in Esasi. We also thank Ryunosuke Irie for technical assistance in phylogenetic analysis, Chengze Li for the maintenance of ‘Bangia’ sp. ESS2, and Yuji Hiwatashi for technical assistance in microscopic observation. Ho Viet Khoa was supported by Ministry of Education, Culture, Sports, Science and Technology of Japan. Puja Kumari is an International Research Fellow supported by JSPS with Postdoctoral Fellowship for Research in Japan (Standard). We also thank Hiroyuki Mizuta for permission of Ho Viet Khoa to study in the Miyagi University

PY - 2021/8/22

Y1 - 2021/8/22

N2 - The red alga ‘Bangia’ sp. ESS1, a ‘Bangia’ 2 clade member, responds to heat stress via accelerated asexual reproduction and acquires thermotolerance based on heat-stress memory. However, whether these strategies are specific to ‘Bangia’ 2, especially ‘Bangia’ sp. ESS1, or whether they are employed by all ‘Bangia’ species is currently unknown. Here, we examined the heat-stress responses of ‘Bangia’ sp. ESS2, a newly identified ‘Bangia’ clade 3 member, and Bangia atropurpurea. Intrinsic thermotolerance differed among species: Whereas ‘Bangia’ sp. ESS1 survived at 30 °C for 7 days, ‘Bangia’ sp. ESS2 and B. atropurpurea did not, with B. atropurpurea showing the highest heat sensitivity. Under sublethal heat stress, the release of asexual spores was highly repressed in ‘Bangia’ sp. ESS2 and completely repressed in B. atropurpurea, whereas it was enhanced in ‘Bangia’ sp. ESS1. ‘Bangia’ sp. ESS2 failed to acquire heat-stress tolerance under sublethal heat-stress conditions, whereas the acquisition of heat tolerance by priming with sublethal high temperatures was observed in both B. atropurpurea and ‘Bangia’ sp. ESS1. Finally, unlike ‘Bangia’ sp. ESS1, neither ‘Bangia’ sp. ESS2 nor B. atropurpurea acquired heat-stress memory. These findings provide insights into the diverse heat-stress response strategies among species from different clades of ‘Bangia’.

AB - The red alga ‘Bangia’ sp. ESS1, a ‘Bangia’ 2 clade member, responds to heat stress via accelerated asexual reproduction and acquires thermotolerance based on heat-stress memory. However, whether these strategies are specific to ‘Bangia’ 2, especially ‘Bangia’ sp. ESS1, or whether they are employed by all ‘Bangia’ species is currently unknown. Here, we examined the heat-stress responses of ‘Bangia’ sp. ESS2, a newly identified ‘Bangia’ clade 3 member, and Bangia atropurpurea. Intrinsic thermotolerance differed among species: Whereas ‘Bangia’ sp. ESS1 survived at 30 °C for 7 days, ‘Bangia’ sp. ESS2 and B. atropurpurea did not, with B. atropurpurea showing the highest heat sensitivity. Under sublethal heat stress, the release of asexual spores was highly repressed in ‘Bangia’ sp. ESS2 and completely repressed in B. atropurpurea, whereas it was enhanced in ‘Bangia’ sp. ESS1. ‘Bangia’ sp. ESS2 failed to acquire heat-stress tolerance under sublethal heat-stress conditions, whereas the acquisition of heat tolerance by priming with sublethal high temperatures was observed in both B. atropurpurea and ‘Bangia’ sp. ESS1. Finally, unlike ‘Bangia’ sp. ESS1, neither ‘Bangia’ sp. ESS2 nor B. atropurpurea acquired heat-stress memory. These findings provide insights into the diverse heat-stress response strategies among species from different clades of ‘Bangia’.

KW - Bangia atropurpurea

KW - ‘Bangia’ sp

KW - heat stress

KW - asexual reproduction

KW - stress memory

KW - thermotolerance

KW - ‘Bangia’ sp.

U2 - 10.3390/plants10081733

DO - 10.3390/plants10081733

M3 - Article

SN - 2223-7747

VL - 10

JO - Plants

JF - Plants

IS - 8

M1 - 1733

ER -

Heat-Stress Responses Differ among Species from Different ‘Bangia’ Clades of Bangiales (Rhodophyta)

Abstract

Bibliographical note

Keywords

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