High dietary protein and fat contents exacerbate hepatic senescence and SASP in mice

Jamil Nehme; Dengbao Yang; Abdullah Altulea; Marta Varela-Eirin; Lu Wang; Sumei Hu; Yingga Wu; Jacques Togo; Chaoqun Niu; John R. Speakman; Marco Demaria

doi:10.1111/febs.16292

High dietary protein and fat contents exacerbate hepatic senescence and SASP in mice

Jamil Nehme, Dengbao Yang, Abdullah Altulea, Marta Varela-Eirin, Lu Wang, Sumei Hu, Yingga Wu, Jacques Togo, Chaoqun Niu, John R. Speakman^*, Marco Demaria^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Dietary choices have a profound impact on the aging process. In addition to the total amount of energy intake, macronutrient composition influences both health and lifespan. However, the exact mechanisms by which dietary macronutrients influence onset and progression of age-associated features remain poorly understood. Cellular senescence is a state of stable growth arrest characterized by the secretion of numerous bioactive molecules with pro-inflammatory properties. Accumulation of senescent cells is considered one of the basic mechanisms of aging and an important contributor to chronic inflammation and tissue degeneration. Whether dietary macronutrients affect the accumulation and the phenotype of senescent cells with age is still unknown. Here, we show that feeding on diets with varying ratios of dietary macronutrients for 3 months has a significant effect on different senescence-associated markers in the mouse liver. High protein intake is associated with higher expression levels of the two classical senescence-associated growth arrest genes, p21 and p16. Furthermore, the expression of many pro-inflammatory secretory markers was increased in diets enriched in protein and further enhanced by increases in fat content. These results provide preliminary evidence that dietary macronutrients have a significant influence on senescence markers and merit further investigation.

Original language	English
Pages (from-to)	1340-1347
Number of pages	8
Journal	FEBS Journal
Volume	290
Issue number	5
Early online date	15 Dec 2021
DOIs	https://doi.org/10.1111/febs.16292
Publication status	Published - Mar 2023

Bibliographical note

Funding Information:
JN is supported by the National Council for Scientific Research‐Lebanese University (CNRS‐L) scholarship. MD is supported by grants from the Dutch Cancer Foundation (KWF). The original mouse macronutrient diet project that generated the samples was funded by the Chinese Academy of Sciences strategic program (Grant XDB 13030100).

Publisher Copyright:
© 2021 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

Data Availability Statement

Peer review
The peer review history for this article is available at https://publons.com/publon/10.1111/febs.16292.

Data accessibility
The data that support the findings of this study are available from the corresponding authors (m.demaria@umcg.nl; j.speakman@abdn.co.uk) upon reasonable request.

Keywords

inflammation
macronutrients composition
SASP
Senescence

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Nehme_etal_TFEBSJ_High_dietary_protein_VOR
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Cite this

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title = "High dietary protein and fat contents exacerbate hepatic senescence and SASP in mice",

abstract = "Dietary choices have a profound impact on the aging process. In addition to the total amount of energy intake, macronutrient composition influences both health and lifespan. However, the exact mechanisms by which dietary macronutrients influence onset and progression of age-associated features remain poorly understood. Cellular senescence is a state of stable growth arrest characterized by the secretion of numerous bioactive molecules with pro-inflammatory properties. Accumulation of senescent cells is considered one of the basic mechanisms of aging and an important contributor to chronic inflammation and tissue degeneration. Whether dietary macronutrients affect the accumulation and the phenotype of senescent cells with age is still unknown. Here, we show that feeding on diets with varying ratios of dietary macronutrients for 3 months has a significant effect on different senescence-associated markers in the mouse liver. High protein intake is associated with higher expression levels of the two classical senescence-associated growth arrest genes, p21 and p16. Furthermore, the expression of many pro-inflammatory secretory markers was increased in diets enriched in protein and further enhanced by increases in fat content. These results provide preliminary evidence that dietary macronutrients have a significant influence on senescence markers and merit further investigation.",

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author = "Jamil Nehme and Dengbao Yang and Abdullah Altulea and Marta Varela-Eirin and Lu Wang and Sumei Hu and Yingga Wu and Jacques Togo and Chaoqun Niu and Speakman, {John R.} and Marco Demaria",

note = "Funding Information: JN is supported by the National Council for Scientific Research‐Lebanese University (CNRS‐L) scholarship. MD is supported by grants from the Dutch Cancer Foundation (KWF). The original mouse macronutrient diet project that generated the samples was funded by the Chinese Academy of Sciences strategic program (Grant XDB 13030100). Publisher Copyright: {\textcopyright} 2021 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.",

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AU - Hu, Sumei

AU - Wu, Yingga

AU - Togo, Jacques

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AU - Speakman, John R.

AU - Demaria, Marco

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N2 - Dietary choices have a profound impact on the aging process. In addition to the total amount of energy intake, macronutrient composition influences both health and lifespan. However, the exact mechanisms by which dietary macronutrients influence onset and progression of age-associated features remain poorly understood. Cellular senescence is a state of stable growth arrest characterized by the secretion of numerous bioactive molecules with pro-inflammatory properties. Accumulation of senescent cells is considered one of the basic mechanisms of aging and an important contributor to chronic inflammation and tissue degeneration. Whether dietary macronutrients affect the accumulation and the phenotype of senescent cells with age is still unknown. Here, we show that feeding on diets with varying ratios of dietary macronutrients for 3 months has a significant effect on different senescence-associated markers in the mouse liver. High protein intake is associated with higher expression levels of the two classical senescence-associated growth arrest genes, p21 and p16. Furthermore, the expression of many pro-inflammatory secretory markers was increased in diets enriched in protein and further enhanced by increases in fat content. These results provide preliminary evidence that dietary macronutrients have a significant influence on senescence markers and merit further investigation.

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High dietary protein and fat contents exacerbate hepatic senescence and SASP in mice

Abstract

Bibliographical note

Data Availability Statement

Keywords

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