Abstract
Many Candida species, such as the opportunistic human pathogen Candida albicans, are Crabtree-Negative yeasts and are therefore highly dependent on the energy generated through oxidative phosphorylation. Respiration control is linked to a range of aspects of C. albicans cell physiology that appear to be important for virulence, most notably its ability to switch from yeast to hyphal forms and the maintenance of the cell wall. The following protocol allows for the measurement and characterization of respiration in C. albicans using high resolution respirometry. We outline how addition of respiration inhibitors can be used to assay the "mode" of respiration, mitochondrial health and the level of electron transport that is coupled to ATP synthase activity in living cell cultures. These data provide useful insight into the effects of external factors, such as exposure to anti-fungal compounds, or internal changes such as genetic alterations on respiratory performance.
Original language | English |
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Article number | e3361 |
Number of pages | 8 |
Journal | Bio-protocol |
Volume | 9 |
Issue number | 17 |
DOIs | |
Publication status | Published - 5 Sept 2019 |
Bibliographical note
This work was supported by the Wellcome Trust Strategic Award for Medical Mycology and FungalImmunology 097377/Z/11/Z
Keywords
- Candida
- Metabolism
- Mitochondria
- Respiration
- Respirometry
- Yeast