High Resolution Respirometry in Candida albicans

Lucian Duvenage, Carol A. Munro, Campbell W. Gourlay* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Many Candida species, such as the opportunistic human pathogen Candida albicans, are Crabtree-Negative yeasts and are therefore highly dependent on the energy generated through oxidative phosphorylation. Respiration control is linked to a range of aspects of C. albicans cell physiology that appear to be important for virulence, most notably its ability to switch from yeast to hyphal forms and the maintenance of the cell wall. The following protocol allows for the measurement and characterization of respiration in C. albicans using high resolution respirometry. We outline how addition of respiration inhibitors can be used to assay the "mode" of respiration, mitochondrial health and the level of electron transport that is coupled to ATP synthase activity in living cell cultures. These data provide useful insight into the effects of external factors, such as exposure to anti-fungal compounds, or internal changes such as genetic alterations on respiratory performance.

Original languageEnglish
Article numbere3361
Number of pages8
Issue number17
Publication statusPublished - 5 Sept 2019

Bibliographical note

This work was supported by the Wellcome Trust Strategic Award for Medical Mycology and Fungal
Immunology 097377/Z/11/Z


  • Candida
  • Metabolism
  • Mitochondria
  • Respiration
  • Respirometry
  • Yeast


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