Hydrodynamic stretching for prostate cancer detection

Yuri Belotti; Michael Conneely; Scott Palmer; Tianjun Huang; Paul Campbell; Stephen McKenna; Ghulam Nabi; David McGloin

doi:10.1117/12.2179201

Hydrodynamic stretching for prostate cancer detection

Yuri Belotti, Michael Conneely, Scott Palmer, Tianjun Huang, Paul Campbell, Stephen McKenna, Ghulam Nabi, David McGloin

Physics

University of Dundee

Research output: Chapter in Book/Report/Conference proceeding › Published conference contribution

1 Citation (Scopus)

Abstract

Advances in diagnostic technologies enabled scientists to link a large number of diseases with structural changes of the intracellular organisation. This intrinsic biophysical characteristic opened up the possibility to perform clinical assessments based on the measurement of single-cell mechanical properties. In this work, we combine microfluidics, high speed imaging and computational automatic tracking to measure the single-cell deformability of large samples of prostate cancer cells at a rate of ∼ 10⁴cells/s. Such a high throughput accounts for the inherent heterogeneity of biological samples and enabled us to extract statistically meaningful signatures from each cell population. In addition, using our technique we investigate the effect of Latrunculin A to the cellular stiffness.

Original language	English
Title of host publication	Bio-MEMS and Medical Microdevices II
Editors	Sander van den Driesche
Publisher	SPIE
ISBN (Electronic)	9781628416411
DOIs	https://doi.org/10.1117/12.2179201
Publication status	Published - 2015
Event	Bio-MEMS and Medical Microdevices II Conference - Barcelona, Spain Duration: 5 May 2015 → 6 May 2015

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9518
ISSN (Print)	1605-7422

Conference

Conference	Bio-MEMS and Medical Microdevices II Conference
Country/Territory	Spain
City	Barcelona
Period	5/05/15 → 6/05/15

Bibliographical note

Publisher Copyright:
© 2015 SPIE.

Keywords

Automated Tracking
Cell Mechanics
High Speed Imaging
High Throughput Diagnosis
Microfluidics
Prostate Cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1117/12.2179201

Cite this

Hydrodynamic stretching for prostate cancer detection. / Belotti, Yuri; Conneely, Michael; Palmer, Scott et al.
Bio-MEMS and Medical Microdevices II. ed. / Sander van den Driesche. SPIE, 2015. 95180H (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9518).

Research output: Chapter in Book/Report/Conference proceeding › Published conference contribution

Belotti, Y, Conneely, M, Palmer, S, Huang, T, Campbell, P, McKenna, S, Nabi, G & McGloin, D 2015, Hydrodynamic stretching for prostate cancer detection. in S van den Driesche (ed.), Bio-MEMS and Medical Microdevices II., 95180H, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9518, SPIE, Bio-MEMS and Medical Microdevices II Conference, Barcelona, Spain, 5/05/15. https://doi.org/10.1117/12.2179201

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keywords = "Automated Tracking, Cell Mechanics, High Speed Imaging, High Throughput Diagnosis, Microfluidics, Prostate Cancer",

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AU - Belotti, Yuri

AU - Conneely, Michael

AU - Palmer, Scott

AU - Huang, Tianjun

AU - Campbell, Paul

AU - McKenna, Stephen

AU - Nabi, Ghulam

AU - McGloin, David

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AB - Advances in diagnostic technologies enabled scientists to link a large number of diseases with structural changes of the intracellular organisation. This intrinsic biophysical characteristic opened up the possibility to perform clinical assessments based on the measurement of single-cell mechanical properties. In this work, we combine microfluidics, high speed imaging and computational automatic tracking to measure the single-cell deformability of large samples of prostate cancer cells at a rate of ∼ 104cells/s. Such a high throughput accounts for the inherent heterogeneity of biological samples and enabled us to extract statistically meaningful signatures from each cell population. In addition, using our technique we investigate the effect of Latrunculin A to the cellular stiffness.

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KW - Cell Mechanics

KW - High Speed Imaging

KW - High Throughput Diagnosis

KW - Microfluidics

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BT - Bio-MEMS and Medical Microdevices II

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Hydrodynamic stretching for prostate cancer detection

Abstract

Publication series

Conference

Bibliographical note

Keywords

UN SDGs

Access to Document

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Cite this