Increased parasite resistance of greater amberjack (Serbia dumerili Risso 1810) juveniles fed a cMOS supplemented diet is associated with upregulation of a discrete set of immune genes in mucosal tissues

Álvaro Fernández-Montero; Silvia Torrecillas; Marisol Izquierdo; María José Caballero; Douglas John Milne; Christopher John Secombes; John Sweetman; Polyana Da Silva; Félix Acosta; Daniel Montero

doi:10.1016/j.fsi.2018.10.034

Increased parasite resistance of greater amberjack (Serbia dumerili Risso 1810) juveniles fed a cMOS supplemented diet is associated with upregulation of a discrete set of immune genes in mucosal tissues

Álvaro Fernández-Montero, Silvia Torrecillas, Marisol Izquierdo, María José Caballero, Douglas John Milne, Christopher John Secombes, John Sweetman, Polyana Da Silva, Félix Acosta, Daniel Montero

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Abstract

The main objective of this study was to determine the effect of two forms of mannan oligosaccharides (MOS: Bio-Mos® and cMOS: Actigen®, Alltech Inc, USA) and their combination on greater amberjack (Seriola dumerili) growth performance and feed efficiency, immune parameters and resistance against ectoparasite (Neobenedenia girellae) infection. Fish were fed for 90 days with 5 g kg-1 MOS, 2 g kg-1 cMOS or a combination of both prebiotics, in a Seriola commercial base diet (Skretting, Norway). At the end of the feeding period, no differences were found in growth performance or feed efficiency. Inclusion of MOS also had no effect on lysozyme activity in skin mucus and serum, but the supplementation of diets with cMOS induced a significant increase of serum bactericidal activity. Dietary cMOS also reduced significantly greater amberjack skin parasite levels, parasite total length and the number of parasites detected per unit of fish surface following a cohabitation challenge with N. girellae, whereas no effect of MOS was detected on these parameters. Of 17 immune genes studied cMOS dietary inclusion up-regulated hepcidin, defensin, Mx protein, interferon-γ (IFNγ), mucin-2 (MUC-2), interleukin-1β (IL-1B), IL-10 and immunoglobulin-T (IgT) gene expression in gills and/or skin. MOS supplementation had a larger impact on spleen and head kidney gene expression, where piscidin, defensin, iNOS, Mx protein, interferons, IL-1β, IL-10, IL-17 and IL-22 were all upregulated. In posterior gut dietary MOS and cMOS both induced IL-10, IgM and IgT, but with MOS also increasing piscidin, MUC-2, and IL-1β whilst cMOS induced hepcidin, defensin and IFNγ. In general, the combination of MOS and cMOS resulted in fewer or lower increases in all tissues, possibly due to an overstimulation effect. The utilization of cMOS at the dose used here has clear benefits on parasite resistance in greater amberjack, linked to upregulation of a discrete set of immune genes in mucosal tissues.

Original language	English
Pages (from-to)	35-45
Number of pages	11
Journal	Fish & Shellfish Immunology
Volume	86
Early online date	16 Oct 2018
DOIs	https://doi.org/10.1016/j.fsi.2018.10.034
Publication status	Published - Mar 2019

Bibliographical note

This work has been cofinanced by the “Agencia Canaria de investigación, Innovación y Sociedad de la Información de la Consejería de Economía, Industria, Comercio y Conocimiento” and the European social funding, “Programa Operativo Integrado de Canarias 2014-2020”, as funding for the PhD grant of AFM. This project also received funding from the European Union Seventh Framework Programme for research, technological development and demonstration (KBBE-2013-07 single stage, GA 603121, DIVERSIFY).

Keywords

MOS
prebiotics
MALT
amberjack
ectoparasites
cytokines
Ectoparasites
Cytokines
Amberjack
Prebiotics
MANNAN OLIGOSACCHARIDES
FEED-UTILIZATION
RAINBOW-TROUT
GIRELLAE MONOGENEA INFECTION
BODY-COMPOSITION
LIVER HISTOLOGY
GROWTH-PERFORMANCE
FISH
SERIOLA-DUMERILI
ATLANTIC SALMON

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Cite this

Fernández-Montero, Á., Torrecillas, S., Izquierdo, M., Caballero, M. J., Milne, D. J., Secombes, C. J., Sweetman, J., Da Silva, P., Acosta, F., & Montero, D. (2019). Increased parasite resistance of greater amberjack (Serbia dumerili Risso 1810) juveniles fed a cMOS supplemented diet is associated with upregulation of a discrete set of immune genes in mucosal tissues. Fish & Shellfish Immunology, 86, 35-45. https://doi.org/10.1016/j.fsi.2018.10.034

Increased parasite resistance of greater amberjack (Serbia dumerili Risso 1810) juveniles fed a cMOS supplemented diet is associated with upregulation of a discrete set of immune genes in mucosal tissues. / Fernández-Montero, Álvaro; Torrecillas, Silvia; Izquierdo, Marisol et al.
In: Fish & Shellfish Immunology, Vol. 86, 03.2019, p. 35-45.

Research output: Contribution to journal › Article › peer-review

Fernández-Montero, Á, Torrecillas, S, Izquierdo, M, Caballero, MJ, Milne, DJ, Secombes, CJ, Sweetman, J, Da Silva, P, Acosta, F & Montero, D 2019, 'Increased parasite resistance of greater amberjack (Serbia dumerili Risso 1810) juveniles fed a cMOS supplemented diet is associated with upregulation of a discrete set of immune genes in mucosal tissues', Fish & Shellfish Immunology, vol. 86, pp. 35-45. https://doi.org/10.1016/j.fsi.2018.10.034

Fernández-Montero Á, Torrecillas S, Izquierdo M, Caballero MJ, Milne DJ, Secombes CJ et al. Increased parasite resistance of greater amberjack (Serbia dumerili Risso 1810) juveniles fed a cMOS supplemented diet is associated with upregulation of a discrete set of immune genes in mucosal tissues. Fish & Shellfish Immunology. 2019 Mar;86:35-45. Epub 2018 Oct 16. doi: 10.1016/j.fsi.2018.10.034

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title = "Increased parasite resistance of greater amberjack (Serbia dumerili Risso 1810) juveniles fed a cMOS supplemented diet is associated with upregulation of a discrete set of immune genes in mucosal tissues",

abstract = "The main objective of this study was to determine the effect of two forms of mannan oligosaccharides (MOS: Bio-Mos{\textregistered} and cMOS: Actigen{\textregistered}, Alltech Inc, USA) and their combination on greater amberjack (Seriola dumerili) growth performance and feed efficiency, immune parameters and resistance against ectoparasite (Neobenedenia girellae) infection. Fish were fed for 90 days with 5 g kg-1 MOS, 2 g kg-1 cMOS or a combination of both prebiotics, in a Seriola commercial base diet (Skretting, Norway). At the end of the feeding period, no differences were found in growth performance or feed efficiency. Inclusion of MOS also had no effect on lysozyme activity in skin mucus and serum, but the supplementation of diets with cMOS induced a significant increase of serum bactericidal activity. Dietary cMOS also reduced significantly greater amberjack skin parasite levels, parasite total length and the number of parasites detected per unit of fish surface following a cohabitation challenge with N. girellae, whereas no effect of MOS was detected on these parameters. Of 17 immune genes studied cMOS dietary inclusion up-regulated hepcidin, defensin, Mx protein, interferon-γ (IFNγ), mucin-2 (MUC-2), interleukin-1β (IL-1B), IL-10 and immunoglobulin-T (IgT) gene expression in gills and/or skin. MOS supplementation had a larger impact on spleen and head kidney gene expression, where piscidin, defensin, iNOS, Mx protein, interferons, IL-1β, IL-10, IL-17 and IL-22 were all upregulated. In posterior gut dietary MOS and cMOS both induced IL-10, IgM and IgT, but with MOS also increasing piscidin, MUC-2, and IL-1β whilst cMOS induced hepcidin, defensin and IFNγ. In general, the combination of MOS and cMOS resulted in fewer or lower increases in all tissues, possibly due to an overstimulation effect. The utilization of cMOS at the dose used here has clear benefits on parasite resistance in greater amberjack, linked to upregulation of a discrete set of immune genes in mucosal tissues.",

keywords = "MOS, prebiotics, MALT, amberjack, ectoparasites, cytokines, Ectoparasites, Cytokines, Amberjack, Prebiotics, MANNAN OLIGOSACCHARIDES, FEED-UTILIZATION, RAINBOW-TROUT, GIRELLAE MONOGENEA INFECTION, BODY-COMPOSITION, LIVER HISTOLOGY, GROWTH-PERFORMANCE, FISH, SERIOLA-DUMERILI, ATLANTIC SALMON",

author = "{\'A}lvaro Fern{\'a}ndez-Montero and Silvia Torrecillas and Marisol Izquierdo and Caballero, {Mar{\'i}a Jos{\'e}} and Milne, {Douglas John} and Secombes, {Christopher John} and John Sweetman and {Da Silva}, Polyana and F{\'e}lix Acosta and Daniel Montero",

note = "This work has been cofinanced by the “Agencia Canaria de investigaci{\'o}n, Innovaci{\'o}n y Sociedad de la Informaci{\'o}n de la Consejer{\'i}a de Econom{\'i}a, Industria, Comercio y Conocimiento” and the European social funding, “Programa Operativo Integrado de Canarias 2014-2020”, as funding for the PhD grant of AFM. This project also received funding from the European Union Seventh Framework Programme for research, technological development and demonstration (KBBE-2013-07 single stage, GA 603121, DIVERSIFY).",

year = "2019",

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doi = "10.1016/j.fsi.2018.10.034",

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T1 - Increased parasite resistance of greater amberjack (Serbia dumerili Risso 1810) juveniles fed a cMOS supplemented diet is associated with upregulation of a discrete set of immune genes in mucosal tissues

AU - Fernández-Montero, Álvaro

AU - Torrecillas, Silvia

AU - Izquierdo, Marisol

AU - Caballero, María José

AU - Milne, Douglas John

AU - Secombes, Christopher John

AU - Sweetman, John

AU - Da Silva, Polyana

AU - Acosta, Félix

AU - Montero, Daniel

N1 - This work has been cofinanced by the “Agencia Canaria de investigación, Innovación y Sociedad de la Información de la Consejería de Economía, Industria, Comercio y Conocimiento” and the European social funding, “Programa Operativo Integrado de Canarias 2014-2020”, as funding for the PhD grant of AFM. This project also received funding from the European Union Seventh Framework Programme for research, technological development and demonstration (KBBE-2013-07 single stage, GA 603121, DIVERSIFY).

PY - 2019/3

Y1 - 2019/3

N2 - The main objective of this study was to determine the effect of two forms of mannan oligosaccharides (MOS: Bio-Mos® and cMOS: Actigen®, Alltech Inc, USA) and their combination on greater amberjack (Seriola dumerili) growth performance and feed efficiency, immune parameters and resistance against ectoparasite (Neobenedenia girellae) infection. Fish were fed for 90 days with 5 g kg-1 MOS, 2 g kg-1 cMOS or a combination of both prebiotics, in a Seriola commercial base diet (Skretting, Norway). At the end of the feeding period, no differences were found in growth performance or feed efficiency. Inclusion of MOS also had no effect on lysozyme activity in skin mucus and serum, but the supplementation of diets with cMOS induced a significant increase of serum bactericidal activity. Dietary cMOS also reduced significantly greater amberjack skin parasite levels, parasite total length and the number of parasites detected per unit of fish surface following a cohabitation challenge with N. girellae, whereas no effect of MOS was detected on these parameters. Of 17 immune genes studied cMOS dietary inclusion up-regulated hepcidin, defensin, Mx protein, interferon-γ (IFNγ), mucin-2 (MUC-2), interleukin-1β (IL-1B), IL-10 and immunoglobulin-T (IgT) gene expression in gills and/or skin. MOS supplementation had a larger impact on spleen and head kidney gene expression, where piscidin, defensin, iNOS, Mx protein, interferons, IL-1β, IL-10, IL-17 and IL-22 were all upregulated. In posterior gut dietary MOS and cMOS both induced IL-10, IgM and IgT, but with MOS also increasing piscidin, MUC-2, and IL-1β whilst cMOS induced hepcidin, defensin and IFNγ. In general, the combination of MOS and cMOS resulted in fewer or lower increases in all tissues, possibly due to an overstimulation effect. The utilization of cMOS at the dose used here has clear benefits on parasite resistance in greater amberjack, linked to upregulation of a discrete set of immune genes in mucosal tissues.

AB - The main objective of this study was to determine the effect of two forms of mannan oligosaccharides (MOS: Bio-Mos® and cMOS: Actigen®, Alltech Inc, USA) and their combination on greater amberjack (Seriola dumerili) growth performance and feed efficiency, immune parameters and resistance against ectoparasite (Neobenedenia girellae) infection. Fish were fed for 90 days with 5 g kg-1 MOS, 2 g kg-1 cMOS or a combination of both prebiotics, in a Seriola commercial base diet (Skretting, Norway). At the end of the feeding period, no differences were found in growth performance or feed efficiency. Inclusion of MOS also had no effect on lysozyme activity in skin mucus and serum, but the supplementation of diets with cMOS induced a significant increase of serum bactericidal activity. Dietary cMOS also reduced significantly greater amberjack skin parasite levels, parasite total length and the number of parasites detected per unit of fish surface following a cohabitation challenge with N. girellae, whereas no effect of MOS was detected on these parameters. Of 17 immune genes studied cMOS dietary inclusion up-regulated hepcidin, defensin, Mx protein, interferon-γ (IFNγ), mucin-2 (MUC-2), interleukin-1β (IL-1B), IL-10 and immunoglobulin-T (IgT) gene expression in gills and/or skin. MOS supplementation had a larger impact on spleen and head kidney gene expression, where piscidin, defensin, iNOS, Mx protein, interferons, IL-1β, IL-10, IL-17 and IL-22 were all upregulated. In posterior gut dietary MOS and cMOS both induced IL-10, IgM and IgT, but with MOS also increasing piscidin, MUC-2, and IL-1β whilst cMOS induced hepcidin, defensin and IFNγ. In general, the combination of MOS and cMOS resulted in fewer or lower increases in all tissues, possibly due to an overstimulation effect. The utilization of cMOS at the dose used here has clear benefits on parasite resistance in greater amberjack, linked to upregulation of a discrete set of immune genes in mucosal tissues.

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KW - prebiotics

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KW - LIVER HISTOLOGY

KW - GROWTH-PERFORMANCE

KW - FISH

KW - SERIOLA-DUMERILI

KW - ATLANTIC SALMON

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ER -

Increased parasite resistance of greater amberjack (Serbia dumerili Risso 1810) juveniles fed a cMOS supplemented diet is associated with upregulation of a discrete set of immune genes in mucosal tissues

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Keywords

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