Increased Variation in Body Weight and Food Intake Is Related to Increased Dietary Fat but Not Increased Carbohydrate or Protein in Mice

Yingga Wu, Sumei Hu, Dengbao Yang, Li Li, Baoguo Li, Lu Wang, Min Li, Guanlin Wang, Jianbo Li, Yanchao Xu, Xueying Zhang, Chaoqun Niu, John R Speakman* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
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A variety of inbred mouse strains have been used for research in metabolic disorders. Despite being inbred, they display large inter-individual variability for many traits like food intake and body weight. However, the relationship between dietary macronutrients and inter-individual variation in body weight and food intake of different mouse strains is still unclear. We investigated the association between macronutrient content of the diet and variations in food intake, body composition, and glucose tolerance by exposing five different mouse strains (C57BL/6, BALB/c, C3H, DBA/2, and FVB) to 24 different diets with variable protein, fat, and carbohydrate contents. We found only increasing dietary fat, but not protein or carbohydrate had a significant association (positive) with variation in both food intake and body weight. The highest variation in both body weight and food intake occurred with 50% dietary fat. However, there were no significant relationships between the variation in fat and lean mass with dietary protein, fat, or carbohydrate levels. In addition, none of the dietary macronutrients had significant impacts on the variation in glucose tolerance ability in C57BL/6 mice. In conclusion, the variations in food intake and body weight changes increased with the elevation of dietary fat levels.

Original languageEnglish
Article number835536
Number of pages9
JournalFrontiers in Nutrition
Publication statusPublished - 8 Mar 2022

Bibliographical note

This study was funded by the National Key R&D Program of China (2019YFA0801900) to JS and the Postdoctoral Innovation Fund (2021) to YW. The original diet exposure experiment was funded by the Chinese Academy of Sciences Strategic Program (XDB13030100). JS was also supported during this work by a PIFI professorial fellowship from CAS and a Wolfson merit award from the UK Royal Society.

CORRECTION article Front. Nutr., 21 October 2022 Sec. Nutrition and Metabolism
Corrigendum: Increased variation in body weight and food intake is related to increased dietary fat but not increased carbohydrate or protein in mice

Data Availability Statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.


  • Protein
  • Fat
  • Carbohydrate
  • Mice
  • Strain
  • Variation


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