Infrared Spectroscopy for Studying Structure and Aging Effects in Rhamnolipid Biosurfactants

Johannes Kiefer*, Mohd Nazren Radzuan, James Winterburn

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)
6 Downloads (Pure)


Biosurfactants are produced by microorganisms and represent amphiphilic compounds with polar and non-polar moieties; hence they can be used to stabilize emulsions, e.g., in the cosmetic and food sectors. Their structure and its changes when exposed to light and elevated temperature are yet to be fully understood. In this study, we demonstrate that attenuated total reflection infrared (ATR-IR) spectroscopy is a useful tool for the analysis of biosurfactants, using rhamnolipids produced by fermentation as an example. A key feature is that the analytical method does not require sample preparation despite the high viscosity of the purified natural product.

Original languageEnglish
Article number533
Number of pages7
JournalApplied Sciences
Issue number5
Publication statusPublished - May 2017


  • vibrational spectroscopy
  • FTIR
  • Pseudomonas aeruginosa
  • rhamnose
  • fatty acid
  • OIL


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