Abstract
Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on phiBT1 integrase were shown to have a broad host range and are fully compatible with those based on the phiC31 attP-int locus.
Original language | English |
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Pages (from-to) | 5320-5323 |
Number of pages | 3 |
Journal | Journal of Bacteriology |
Volume | 185 |
DOIs | |
Publication status | Published - 2003 |
Keywords
- ESCHERICHIA-COLI
- COELICOLOR A3(2)
- SEQUENCE