Distinctions between cell types underpin organisational principles for nervous system function. Functional variation also exists between neurons of the same type. This is exemplified by correspondence between grid cell spatial scales and synaptic integrative properties of stellate cells (SCs) in the medial entorhinal cortex. However, we know little about how functional variability is structured either within or between individuals. Using ex-vivo patch-clamp recordings from up to 55 SCs per mouse, we find that integrative properties vary between mice and, in contrast to modularity of grid cell spatial scales, have a continuous dorsoventral organisation. Our results constrain mechanisms for modular grid firing and provide evidence for inter-animal phenotypic variability among neurons of the same type. We suggest that neuron type properties are tuned to circuit level set points that vary within and between animals.
Bibliographical noteFunding Information:
We thank Vanessa Stempel for comments on the manuscript, Tor Stensola and Edvard Moser for sharing published data, and Lukas Solanka and Lukas Fischer for help with building the large cage. This work was supported by grants to MN from the Wellcome Trust (200855/Z/16/Z) and the BBSRC (BB/L010496/1, BB/1022147/1 and BB/H020284/1).
© 2020, eLife Sciences Publications Ltd. All rights reserved.
Data Availability StatementProcessed data used for analyses and all associated code is available from the GitHub page for the project (https://github.com/MattNolanLab/Inter_Intra_Variation, copy archived at https://github.com/elifesciences-publications/Inter_Intra_Variation). Raw data has been made available from our institutional repository and can be found at https://doi.org/10.7488/ds/2765.
The following data sets were generated
Hugh PDerek LGMatthew FN (2020) Edinburgh DataShare Inter- and intra-animal variation in the integrative properties of stellate cells in the medial entorhinal cortex. https://doi.org/10.7488/ds/2765
- GRID CELLS
- HCN1 CHANNELS