Inter- and intra-animal variation in the integrative properties of stellate cells in the medial entorhinal cortex

Hugh Pastoll; Derek L Garden; Ioannis Papastathopoulos; Gülşen Sürmeli; Matthew F Nolan

doi:10.7554/eLife.52258

Inter- and intra-animal variation in the integrative properties of stellate cells in the medial entorhinal cortex

Hugh Pastoll, Derek L Garden, Ioannis Papastathopoulos, Gülşen Sürmeli, Matthew F Nolan^* (Corresponding Author)

^*Corresponding author for this work

University of Edinburgh

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

2 Downloads (Pure)

Abstract

Distinctions between cell types underpin organisational principles for nervous system function. Functional variation also exists between neurons of the same type. This is exemplified by correspondence between grid cell spatial scales and synaptic integrative properties of stellate cells (SCs) in the medial entorhinal cortex. However, we know little about how functional variability is structured either within or between individuals. Using ex-vivo patch-clamp recordings from up to 55 SCs per mouse, we find that integrative properties vary between mice and, in contrast to modularity of grid cell spatial scales, have a continuous dorsoventral organisation. Our results constrain mechanisms for modular grid firing and provide evidence for inter-animal phenotypic variability among neurons of the same type. We suggest that neuron type properties are tuned to circuit level set points that vary within and between animals.

Original language	English
Article number	e52258
Number of pages	25
Journal	eLife
Volume	9
Early online date	10 Feb 2020
DOIs	https://doi.org/10.7554/eLife.52258
Publication status	Published - Feb 2020
Externally published	Yes

Bibliographical note

Funding Information:
We thank Vanessa Stempel for comments on the manuscript, Tor Stensola and Edvard Moser for sharing published data, and Lukas Solanka and Lukas Fischer for help with building the large cage. This work was supported by grants to MN from the Wellcome Trust (200855/Z/16/Z) and the BBSRC (BB/L010496/1, BB/1022147/1 and BB/H020284/1).

Data Availability Statement

Processed data used for analyses and all associated code is available from the GitHub page for the project (https://github.com/MattNolanLab/Inter_Intra_Variation, copy archived at https://github.com/elifesciences-publications/Inter_Intra_Variation). Raw data has been made available from our institutional repository and can be found at https://doi.org/10.7488/ds/2765.

The following data sets were generated
Hugh PDerek LGMatthew FN (2020) Edinburgh DataShare Inter- and intra-animal variation in the integrative properties of stellate cells in the medial entorhinal cortex. https://doi.org/10.7488/ds/2765

Keywords

DORSAL
FREQUENCY
GRID CELLS
HCN1 CHANNELS
LAYER-II
MODEL
NEURONS
ORGANIZATION
OSCILLATIONS
THETA

Access to Document

10.7554/eLife.52258Licence: CC BY

Pastoll_etal_N_Inter_and_intra-animal_VOR
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. https://creativecommons.org/licenses/by/4.0/
Final published version, 4.25 MBLicence: CC BY

Cite this

@article{05914382e9174f87a862b734fa717e71,

title = "Inter- and intra-animal variation in the integrative properties of stellate cells in the medial entorhinal cortex",

abstract = "Distinctions between cell types underpin organisational principles for nervous system function. Functional variation also exists between neurons of the same type. This is exemplified by correspondence between grid cell spatial scales and synaptic integrative properties of stellate cells (SCs) in the medial entorhinal cortex. However, we know little about how functional variability is structured either within or between individuals. Using ex-vivo patch-clamp recordings from up to 55 SCs per mouse, we find that integrative properties vary between mice and, in contrast to modularity of grid cell spatial scales, have a continuous dorsoventral organisation. Our results constrain mechanisms for modular grid firing and provide evidence for inter-animal phenotypic variability among neurons of the same type. We suggest that neuron type properties are tuned to circuit level set points that vary within and between animals.",

keywords = "DORSAL, FREQUENCY, GRID CELLS, HCN1 CHANNELS, LAYER-II, MODEL, NEURONS, ORGANIZATION, OSCILLATIONS, THETA",

author = "Hugh Pastoll and Garden, {Derek L} and Ioannis Papastathopoulos and G{\"u}l{\c s}en S{\"u}rmeli and Nolan, {Matthew F}",

note = "Funding Information: We thank Vanessa Stempel for comments on the manuscript, Tor Stensola and Edvard Moser for sharing published data, and Lukas Solanka and Lukas Fischer for help with building the large cage. This work was supported by grants to MN from the Wellcome Trust (200855/Z/16/Z) and the BBSRC (BB/L010496/1, BB/1022147/1 and BB/H020284/1). ",

year = "2020",

month = feb,

doi = "10.7554/eLife.52258",

language = "English",

volume = "9",

journal = "eLife",

publisher = "eLife Sciences Publications",

}

TY - JOUR

T1 - Inter- and intra-animal variation in the integrative properties of stellate cells in the medial entorhinal cortex

AU - Pastoll, Hugh

AU - Garden, Derek L

AU - Papastathopoulos, Ioannis

AU - Sürmeli, Gülşen

AU - Nolan, Matthew F

N1 - Funding Information: We thank Vanessa Stempel for comments on the manuscript, Tor Stensola and Edvard Moser for sharing published data, and Lukas Solanka and Lukas Fischer for help with building the large cage. This work was supported by grants to MN from the Wellcome Trust (200855/Z/16/Z) and the BBSRC (BB/L010496/1, BB/1022147/1 and BB/H020284/1).

PY - 2020/2

Y1 - 2020/2

N2 - Distinctions between cell types underpin organisational principles for nervous system function. Functional variation also exists between neurons of the same type. This is exemplified by correspondence between grid cell spatial scales and synaptic integrative properties of stellate cells (SCs) in the medial entorhinal cortex. However, we know little about how functional variability is structured either within or between individuals. Using ex-vivo patch-clamp recordings from up to 55 SCs per mouse, we find that integrative properties vary between mice and, in contrast to modularity of grid cell spatial scales, have a continuous dorsoventral organisation. Our results constrain mechanisms for modular grid firing and provide evidence for inter-animal phenotypic variability among neurons of the same type. We suggest that neuron type properties are tuned to circuit level set points that vary within and between animals.

AB - Distinctions between cell types underpin organisational principles for nervous system function. Functional variation also exists between neurons of the same type. This is exemplified by correspondence between grid cell spatial scales and synaptic integrative properties of stellate cells (SCs) in the medial entorhinal cortex. However, we know little about how functional variability is structured either within or between individuals. Using ex-vivo patch-clamp recordings from up to 55 SCs per mouse, we find that integrative properties vary between mice and, in contrast to modularity of grid cell spatial scales, have a continuous dorsoventral organisation. Our results constrain mechanisms for modular grid firing and provide evidence for inter-animal phenotypic variability among neurons of the same type. We suggest that neuron type properties are tuned to circuit level set points that vary within and between animals.

KW - DORSAL

KW - FREQUENCY

KW - GRID CELLS

KW - HCN1 CHANNELS

KW - LAYER-II

KW - MODEL

KW - NEURONS

KW - ORGANIZATION

KW - OSCILLATIONS

KW - THETA

UR - https://doi.org/10.7554/eLife.52258

UR - http://www.scopus.com/inward/record.url?scp=85081945138&partnerID=8YFLogxK

U2 - 10.7554/eLife.52258

DO - 10.7554/eLife.52258

M3 - Article

VL - 9

JO - eLife

JF - eLife

M1 - e52258

ER -

Inter- and intra-animal variation in the integrative properties of stellate cells in the medial entorhinal cortex

Abstract

Bibliographical note

Data Availability Statement

Keywords

Access to Document

Other files and links

Fingerprint

Cite this