Interaction of whey protein with polyphenols from salal fruits (Gaultheria shallon) and the effects on protein structure and hydrolysis pattern by Flavourzyme®

He Ni* (Corresponding Author), Helen Hayes, David Stead, Guang Liu, Huaijie Yang, Haihang Li, Vassilios Raikos

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
7 Downloads (Pure)

Abstract

Milk whey can interact with polyphenols leading to the formation of complexes. In this research, whey protein was fortified with salal fruits (SB) extract and the effect on protein structure was investigated. Particle size and tertiary structure analysis indicates α‐Lactalbumin‐ligand interactions when whey is supplemented with SB extract. Circular dichroism spectroscopy suggests conformational changes of α‐Lac to a partially unfolded state as indicated by the decrease in α‐helix structures. Enzymatic treatment of whey protein mixed with SB revealed differences in the hydrolysis pattern. LC‐MS/MS data analysis indicates that a higher number of peptides are released when whey is mixed with SB. Peptides of known bioactivity were identified in all hydrolysates. The supplementation of whey protein with SB extract can influence protein hydrolysis and the release of peptides following enzymatic treatment with commercial proteases which may affect the functional and health related properties of the hydrolysate.

Original languageEnglish
Pages (from-to)1281-1288
Number of pages8
JournalInternational Journal of Food Science and Technology
Volume55
Issue number3
Early online date22 Oct 2019
DOIs
Publication statusPublished - 1 Mar 2020

Bibliographical note

This work is part of the Strategic Research Programme 2016-2021 and is funded by the Scottish Government's Rural and Environment Science and Analytical Services Division (RESAS). This work was also supported by Guangdong Provincial Science and Technology Program (No. 2017A030310031).

Keywords

  • whey protein
  • salal berry
  • Flavourzyme®
  • bioactive peptides
  • interaction
  • BIOACTIVE PEPTIDES
  • ACID
  • Flavourzyme (R)
  • ANTIOXIDANT PROPERTIES
  • CONCENTRATE
  • OPTIMIZATION
  • Bioactive peptides
  • MILK
  • Flavourzyme

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