Intraerythrocytic stages of Plasmodium falciparum biosynthesize menaquinone

Renata Tonhosolo; Heloisa B Gabriel; Miriam Y Matsumura; Fernanda J Cabral; Márcio M Yamamoto; Fábio L D'Alexandri; Rodrigo A C Sussmann; Rodrigo Belmonte; Valnice J Peres; Dean C Crick; Gerhard Wunderlich; Emília A Kimura; Alejandro M Katzin

doi:10.1016/j.febslet.2010.10.055

Intraerythrocytic stages of Plasmodium falciparum biosynthesize menaquinone

Renata Tonhosolo, Heloisa B Gabriel, Miriam Y Matsumura, Fernanda J Cabral, Márcio M Yamamoto, Fábio L D'Alexandri, Rodrigo A C Sussmann, Rodrigo Belmonte, Valnice J Peres, Dean C Crick, Gerhard Wunderlich, Emília A Kimura, Alejandro M Katzin

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Herein, we show that intraerythrocytic stages of Plasmodium falciparum have an active pathway for biosynthesis of menaquinone. Kinetic assays confirmed that plasmodial menaquinone acts at least in the electron transport. Similarly to Escherichia coli, we observed increased levels of menaquinone in parasites kept under anaerobic conditions. Additionally, the mycobacterial inhibitor of menaquinone synthesis Ro 48-8071 also suppressed menaquinone biosynthesis and growth of parasites, although off-targets may play a role in this growth-inhibitory effect. Due to its absence in humans, the menaquinone biosynthesis can be considered an important drug target for malaria.

Original language	English
Pages (from-to)	4761-4768
Number of pages	8
Journal	FEBS Letters
Volume	584
Issue number	23
Early online date	29 Oct 2010
DOIs	https://doi.org/10.1016/j.febslet.2010.10.055
Publication status	Published - Dec 2010

Keywords

anaerobiosis
animals
benzophenones
electrons
erythrocytes
life cycle stages
malaria
molecular targeted therapy
Plasmodium falciparum
vitamin K 2

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.febslet.2010.10.055

Cite this

Tonhosolo, R., Gabriel, H. B., Matsumura, M. Y., Cabral, F. J., Yamamoto, M. M., D'Alexandri, F. L., Sussmann, R. A. C., Belmonte, R., Peres, V. J., Crick, D. C., Wunderlich, G., Kimura, E. A., & Katzin, A. M. (2010). Intraerythrocytic stages of Plasmodium falciparum biosynthesize menaquinone. FEBS Letters, 584(23), 4761-4768. https://doi.org/10.1016/j.febslet.2010.10.055

@article{f8583502b1aa4d9dada6f782f27b0845,

title = "Intraerythrocytic stages of Plasmodium falciparum biosynthesize menaquinone",

abstract = "Herein, we show that intraerythrocytic stages of Plasmodium falciparum have an active pathway for biosynthesis of menaquinone. Kinetic assays confirmed that plasmodial menaquinone acts at least in the electron transport. Similarly to Escherichia coli, we observed increased levels of menaquinone in parasites kept under anaerobic conditions. Additionally, the mycobacterial inhibitor of menaquinone synthesis Ro 48-8071 also suppressed menaquinone biosynthesis and growth of parasites, although off-targets may play a role in this growth-inhibitory effect. Due to its absence in humans, the menaquinone biosynthesis can be considered an important drug target for malaria.",

keywords = "anaerobiosis, animals, benzophenones, electrons, erythrocytes, life cycle stages, malaria, molecular targeted therapy, Plasmodium falciparum, vitamin K 2",

author = "Renata Tonhosolo and Gabriel, {Heloisa B} and Matsumura, {Miriam Y} and Cabral, {Fernanda J} and Yamamoto, {M{\'a}rcio M} and D'Alexandri, {F{\'a}bio L} and Sussmann, {Rodrigo A C} and Rodrigo Belmonte and Peres, {Valnice J} and Crick, {Dean C} and Gerhard Wunderlich and Kimura, {Em{\'i}lia A} and Katzin, {Alejandro M}",

year = "2010",

month = dec,

doi = "10.1016/j.febslet.2010.10.055",

language = "English",

volume = "584",

pages = "4761--4768",

journal = "FEBS Letters",

issn = "1873-3468",

publisher = "Wiley",

number = "23",

}

TY - JOUR

T1 - Intraerythrocytic stages of Plasmodium falciparum biosynthesize menaquinone

AU - Tonhosolo, Renata

AU - Gabriel, Heloisa B

AU - Matsumura, Miriam Y

AU - Cabral, Fernanda J

AU - Yamamoto, Márcio M

AU - D'Alexandri, Fábio L

AU - Sussmann, Rodrigo A C

AU - Belmonte, Rodrigo

AU - Peres, Valnice J

AU - Crick, Dean C

AU - Wunderlich, Gerhard

AU - Kimura, Emília A

AU - Katzin, Alejandro M

PY - 2010/12

Y1 - 2010/12

N2 - Herein, we show that intraerythrocytic stages of Plasmodium falciparum have an active pathway for biosynthesis of menaquinone. Kinetic assays confirmed that plasmodial menaquinone acts at least in the electron transport. Similarly to Escherichia coli, we observed increased levels of menaquinone in parasites kept under anaerobic conditions. Additionally, the mycobacterial inhibitor of menaquinone synthesis Ro 48-8071 also suppressed menaquinone biosynthesis and growth of parasites, although off-targets may play a role in this growth-inhibitory effect. Due to its absence in humans, the menaquinone biosynthesis can be considered an important drug target for malaria.

AB - Herein, we show that intraerythrocytic stages of Plasmodium falciparum have an active pathway for biosynthesis of menaquinone. Kinetic assays confirmed that plasmodial menaquinone acts at least in the electron transport. Similarly to Escherichia coli, we observed increased levels of menaquinone in parasites kept under anaerobic conditions. Additionally, the mycobacterial inhibitor of menaquinone synthesis Ro 48-8071 also suppressed menaquinone biosynthesis and growth of parasites, although off-targets may play a role in this growth-inhibitory effect. Due to its absence in humans, the menaquinone biosynthesis can be considered an important drug target for malaria.

KW - anaerobiosis

KW - animals

KW - benzophenones

KW - electrons

KW - erythrocytes

KW - life cycle stages

KW - malaria

KW - molecular targeted therapy

KW - Plasmodium falciparum

KW - vitamin K 2

U2 - 10.1016/j.febslet.2010.10.055

DO - 10.1016/j.febslet.2010.10.055

M3 - Article

C2 - 21036171

SN - 1873-3468

VL - 584

SP - 4761

EP - 4768

JO - FEBS Letters

JF - FEBS Letters

IS - 23

ER -

Intraerythrocytic stages of Plasmodium falciparum biosynthesize menaquinone

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this