Investigating the role of Candida albicans as a universal substrate for oral bacteria using a transcriptomic approach: implications for interkingdom biofilm control?

Christopher Delaney; Susanth Alapati; Muhanna Alshehri; Dominika Kubalova; Chandra Lekha Ramalingham Veena; Sumaya Abusrewil; Bryn Short; David Bradshaw; Jason L. Brown

doi:10.1111/apm.13327

Investigating the role of Candida albicans as a universal substrate for oral bacteria using a transcriptomic approach: implications for interkingdom biofilm control?

Christopher Delaney^* (Corresponding Author), Susanth Alapati, Muhanna Alshehri, Dominika Kubalova, Chandra Lekha Ramalingham Veena, Sumaya Abusrewil, Bryn Short, David Bradshaw, Jason L. Brown^* (Corresponding Author)

^*Corresponding author for this work

Dental Education

Research output: Contribution to journal › Article › peer-review

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Abstract

Candida albicans is frequently identified as a colonizer of the oral cavity in health and has recently been termed a “keystone” commensal due to its role on the bacterial communities. However, the role that C. albicans plays in such interactions is not fully understood. Therefore, this study aimed to identify the relationship between C. albicans and bacteria associated with oral symbiosis and dysbiosis. To do this, we evaluated the ability of C. albicans to support the growth of the aerobic commensal Streptococcus gordonii and the anaerobic pathogens Fusobacterium nucleatum and Porphyromonas gingivalis in the biofilm environment. RNA-Sequencing with the Illumina platform was then utilized to identify C. albicans gene expression and functional pathways involved during such interactions in dual-species and a 4-species biofilm model. Results indicated that C. albicans was capable of supporting growth of all three bacteria, with a significant increase in colony counts of each bacteria in the dual-species biofilm (p

Original language	English
Journal	APMIS
DOIs	https://doi.org/10.1111/apm.13327
Publication status	Published - 11 May 2023

Bibliographical note

FUNDING INFORMATION
We would like to acknowledge the funding support from the GlaxoSmithKline BBSRC Industrial CASE PhD studentship for CD (BB/P504567/1).

Keywords

Biofilms
Candida Albicans
Streptococcus gordonii
Fusobacterium nucleatum
Porphyromonas gingivalis
fungal-bacterial interactions
RNA-sequencing

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@article{3484a98f9ec24630b1f99669db2d4bd3,

title = "Investigating the role of Candida albicans as a universal substrate for oral bacteria using a transcriptomic approach: implications for interkingdom biofilm control?",

abstract = "Candida albicans is frequently identified as a colonizer of the oral cavity in health and has recently been termed a “keystone” commensal due to its role on the bacterial communities. However, the role that C. albicans plays in such interactions is not fully understood. Therefore, this study aimed to identify the relationship between C. albicans and bacteria associated with oral symbiosis and dysbiosis. To do this, we evaluated the ability of C. albicans to support the growth of the aerobic commensal Streptococcus gordonii and the anaerobic pathogens Fusobacterium nucleatum and Porphyromonas gingivalis in the biofilm environment. RNA-Sequencing with the Illumina platform was then utilized to identify C. albicans gene expression and functional pathways involved during such interactions in dual-species and a 4-species biofilm model. Results indicated that C. albicans was capable of supporting growth of all three bacteria, with a significant increase in colony counts of each bacteria in the dual-species biofilm (p ",

keywords = "Biofilms, Candida Albicans, Streptococcus gordonii, Fusobacterium nucleatum, Porphyromonas gingivalis, fungal-bacterial interactions, RNA-sequencing",

author = "Christopher Delaney and Susanth Alapati and Muhanna Alshehri and Dominika Kubalova and Veena, {Chandra Lekha Ramalingham} and Sumaya Abusrewil and Bryn Short and David Bradshaw and Brown, {Jason L.}",

note = "FUNDING INFORMATION We would like to acknowledge the funding support from the GlaxoSmithKline BBSRC Industrial CASE PhD studentship for CD (BB/P504567/1).",

year = "2023",

month = may,

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doi = "10.1111/apm.13327",

language = "English",

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T2 - implications for interkingdom biofilm control?

AU - Delaney, Christopher

AU - Alapati, Susanth

AU - Alshehri, Muhanna

AU - Kubalova, Dominika

AU - Veena, Chandra Lekha Ramalingham

AU - Abusrewil, Sumaya

AU - Short, Bryn

AU - Bradshaw, David

AU - Brown, Jason L.

N1 - FUNDING INFORMATION We would like to acknowledge the funding support from the GlaxoSmithKline BBSRC Industrial CASE PhD studentship for CD (BB/P504567/1).

PY - 2023/5/11

Y1 - 2023/5/11

N2 - Candida albicans is frequently identified as a colonizer of the oral cavity in health and has recently been termed a “keystone” commensal due to its role on the bacterial communities. However, the role that C. albicans plays in such interactions is not fully understood. Therefore, this study aimed to identify the relationship between C. albicans and bacteria associated with oral symbiosis and dysbiosis. To do this, we evaluated the ability of C. albicans to support the growth of the aerobic commensal Streptococcus gordonii and the anaerobic pathogens Fusobacterium nucleatum and Porphyromonas gingivalis in the biofilm environment. RNA-Sequencing with the Illumina platform was then utilized to identify C. albicans gene expression and functional pathways involved during such interactions in dual-species and a 4-species biofilm model. Results indicated that C. albicans was capable of supporting growth of all three bacteria, with a significant increase in colony counts of each bacteria in the dual-species biofilm (p

AB - Candida albicans is frequently identified as a colonizer of the oral cavity in health and has recently been termed a “keystone” commensal due to its role on the bacterial communities. However, the role that C. albicans plays in such interactions is not fully understood. Therefore, this study aimed to identify the relationship between C. albicans and bacteria associated with oral symbiosis and dysbiosis. To do this, we evaluated the ability of C. albicans to support the growth of the aerobic commensal Streptococcus gordonii and the anaerobic pathogens Fusobacterium nucleatum and Porphyromonas gingivalis in the biofilm environment. RNA-Sequencing with the Illumina platform was then utilized to identify C. albicans gene expression and functional pathways involved during such interactions in dual-species and a 4-species biofilm model. Results indicated that C. albicans was capable of supporting growth of all three bacteria, with a significant increase in colony counts of each bacteria in the dual-species biofilm (p

KW - Biofilms

KW - Candida Albicans

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KW - Fusobacterium nucleatum

KW - Porphyromonas gingivalis

KW - fungal-bacterial interactions

KW - RNA-sequencing

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DO - 10.1111/apm.13327

M3 - Article

SN - 0903-4641

JO - APMIS

JF - APMIS

ER -

Investigating the role of Candida albicans as a universal substrate for oral bacteria using a transcriptomic approach: implications for interkingdom biofilm control?

Abstract

Bibliographical note

Keywords

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