Isolation and characterization of new phenolic siderophores with antimicrobial properties from Pseudomonas sp. UIAU-6B

Emmanuel T. Oluwabusola, Olusoji O. Adebisi, Fernando Reyes, Kojo S. Acquah, Mercedes De la Cruz, Larry L. Mweetwa, Joy E. Rajakulendran, Digby F. Warner, Deng Hai, Rainer Ebel, Marcel Jaspars

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Five new phenolic siderophores 1-5 were isolated from the organic extract of a culture broth in a modified SGG medium of Pseu-domonas sp. UIAU-6B, obtained from sediments collected from the Oyun river in North Central Nigeria. The structure of the new compounds, pseudomonin A-C (1-3) and pseudomobactin A and B (4 and 5) isolated alongside two known compounds, pseudomonine (6) and salicylic acid (7), were elucidated based on high-resolution mass spectrometry, 1D and 2D NMR analyses. The absolute configuration of the threonine residue in compounds 1-5 was determined by Marfey analysis. The antimicrobial eval-uation of compound 4 exhibited the most potent activity against vancomycin-sensitive Enterococcus faecium VS144754, followed by 3 and 5, with MIC values ranging from 8 to 32 mu g/mL. Compounds 2 and 3 exhibited moderate activity against Mycobacterium tuberculosis H37Rv, with MIC values of 7.8 and 15.6 mu g/mL, respectively. Plausible biosynthetic hypotheses toward the new com-pounds 1-5 were proposed.

Original languageEnglish
Pages (from-to)2390-2398
Number of pages9
JournalBeilstein journal of organic chemistry
Publication statusPublished - 13 Sept 2021

Bibliographical note

We thank Russell Gray, Subha Arjuna, and Shan Wang of the Marine Biodiscovery Centre, Aberdeen for assisting in NMR, mass spectrometry and Marfey analyses, respectively. We also appreciate the support of Dr. Soji Fakoya and Blessing Akinboyo of Ondo State University of Science and Technology, Okitipupa, Nigeria, for their support in the collection of sediments.
This work was supported by funding from TETFund, Nigeria for a University Senate Based research.


  • Mycobacterium tuberculosis
  • Phenolic siderophores
  • pseudomonine
  • Pseudomonas sp
  • vancomycin-sensitive Enterococcus faecium


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