Isolation and recombinant expression of an MHV-JHM neutralising monoclonal antibody

Andreas Kolb; M  Lechermaier; A  Heister; A  Toksoy; S G  Siddell

Isolation and recombinant expression of an MHV-JHM neutralising monoclonal antibody

Andreas Kolb, M Lechermaier, A Heister, A Toksoy, S G Siddell

The Rowett Institute of Nutrition and Health

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Abstract

The monoclonal antibody Al (mab Al) efficiently neutralises the infection of susceptible cells by the murine hepatitis virus MHV-JHM in vitro and in vivo (Wege et al., 1984). The variable regions of mab Al were amplified from mRNA of the respective hybridoma cell line by RT-PCR and integrated into different eukaryotic expression vectors. The biological function of the recombinant antibody constructs was verified by virus neutralisation assays. Whereas a complete recombinant antibody (mab Alrec.) expressed in transfected murine myeloma cells inhibited the MHV-JHM infection as well as the parental antibody, a single-chain Fv derived from mab Al did not show any neutralising activity.

Original language	English
Pages (from-to)	657-664
Number of pages	8
Journal	Advances in Experimental Medicine and Biology
Volume	440
Publication status	Published - 1998

Keywords

protein

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Cite this

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title = "Isolation and recombinant expression of an MHV-JHM neutralising monoclonal antibody",

abstract = "The monoclonal antibody Al (mab Al) efficiently neutralises the infection of susceptible cells by the murine hepatitis virus MHV-JHM in vitro and in vivo (Wege et al., 1984). The variable regions of mab Al were amplified from mRNA of the respective hybridoma cell line by RT-PCR and integrated into different eukaryotic expression vectors. The biological function of the recombinant antibody constructs was verified by virus neutralisation assays. Whereas a complete recombinant antibody (mab Alrec.) expressed in transfected murine myeloma cells inhibited the MHV-JHM infection as well as the parental antibody, a single-chain Fv derived from mab Al did not show any neutralising activity.",

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journal = "Advances in Experimental Medicine and Biology",

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T1 - Isolation and recombinant expression of an MHV-JHM neutralising monoclonal antibody

AU - Kolb, Andreas

AU - Lechermaier, M

AU - Heister, A

AU - Toksoy, A

AU - Siddell, S G

PY - 1998

Y1 - 1998

N2 - The monoclonal antibody Al (mab Al) efficiently neutralises the infection of susceptible cells by the murine hepatitis virus MHV-JHM in vitro and in vivo (Wege et al., 1984). The variable regions of mab Al were amplified from mRNA of the respective hybridoma cell line by RT-PCR and integrated into different eukaryotic expression vectors. The biological function of the recombinant antibody constructs was verified by virus neutralisation assays. Whereas a complete recombinant antibody (mab Alrec.) expressed in transfected murine myeloma cells inhibited the MHV-JHM infection as well as the parental antibody, a single-chain Fv derived from mab Al did not show any neutralising activity.

AB - The monoclonal antibody Al (mab Al) efficiently neutralises the infection of susceptible cells by the murine hepatitis virus MHV-JHM in vitro and in vivo (Wege et al., 1984). The variable regions of mab Al were amplified from mRNA of the respective hybridoma cell line by RT-PCR and integrated into different eukaryotic expression vectors. The biological function of the recombinant antibody constructs was verified by virus neutralisation assays. Whereas a complete recombinant antibody (mab Alrec.) expressed in transfected murine myeloma cells inhibited the MHV-JHM infection as well as the parental antibody, a single-chain Fv derived from mab Al did not show any neutralising activity.

KW - protein

M3 - Article

SN - 0065-2598

VL - 440

SP - 657

EP - 664

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

ER -

Isolation and recombinant expression of an MHV-JHM neutralising monoclonal antibody

Abstract

Keywords

UN SDGs

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