Isolation of rainbow trout neutrophils with an anti-granulocyte monoclonal antibody

S. H. Hamdani, D. N. McMillan, E. F. Pettersen, H. Wergeland, C. Endresen, Anthony E. Ellis, C. J. Secombes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


A magnetic cell sorting system has been optimised for the purification of rainbow trout neutrophils using a monoclonal antibody (E3D9) raised against Atlantic salmon neutrophils. The purified neutrophils have good viability (85%) and purity (~92%), and were functional in respiratory burst and migration assays. The isolated neutrophils responded rapidly to PMA stimulation, producing levels of superoxide anion (4.85 nmols superoxide min-1/106 cells) approximately twice as high as macrophages from the same species. In the migration assay, there was a four-fold increase in migrating cells using the purified neutrophils compared with unfractionated blood leucocytes, and a relatively high neutrophil migratory activity was seen in the absence of serum.

Original languageEnglish
Pages (from-to)369-380
Number of pages12
JournalVeterinary Immunology and Immunopathology
Issue number4
Publication statusPublished - 12 Jun 1998


  • Eosinophilic granular cells
  • Migration
  • Monoclonal antibody
  • Neutrophils
  • Rainbow trout
  • Respiratory burst


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