Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3

M. Mutocheluh; L. Hindle; C. Aresté; S. A. Chanas; L. M. Butler; K. Lowry; K. Shah; D. J. Evans; D. J. Blackbourn

doi:10.1099/vir.0.034322-0

Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3

M. Mutocheluh, L. Hindle, C. Aresté, S. A. Chanas, L. M. Butler, K. Lowry, K. Shah, D. J. Evans, D. J. Blackbourn

Medical Sciences

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33 Citations (Scopus)

Abstract

Kaposi's sarcoma-associated herpesvirus (KSHV) encodes four viral interferon regulatory factors (vIRF-1-4). We investigated the mechanism and consequences of vIRF-2-mediated inhibition of interferon-response element signalling following type I interferon (IFN) induction. Western blot and electrophoretic mobility-shift assays identified the interferon-stimulated gene factor-3 (ISGF-3) components STAT1 and IRF-9 as the proximal targets of vIRF-2 activity. The biological significance of vIRF-2 inhibition of ISGF-3 was demonstrated by vIRF-2-mediated rescue of the replication of the IFN-sensitive virus encephalomyocarditis virus. This study provides both a mechanism and evidence for KSHV vIRF-2-mediated suppression of the consequences of type 1 IFN-induced signaling.

Original language	English
Pages (from-to)	2394-2398
Number of pages	5
Journal	Journal of General Virology
Volume	92
Issue number	10
DOIs	https://doi.org/10.1099/vir.0.034322-0
Publication status	Published - Oct 2011

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Mutocheluh, M., Hindle, L., Aresté, C., Chanas, S. A., Butler, L. M., Lowry, K., Shah, K., Evans, D. J., & Blackbourn, D. J. (2011). Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3. Journal of General Virology, 92(10), 2394-2398. https://doi.org/10.1099/vir.0.034322-0

Mutocheluh, M, Hindle, L, Aresté, C, Chanas, SA, Butler, LM, Lowry, K, Shah, K, Evans, DJ & Blackbourn, DJ 2011, 'Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3', Journal of General Virology, vol. 92, no. 10, pp. 2394-2398. https://doi.org/10.1099/vir.0.034322-0

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abstract = "Kaposi's sarcoma-associated herpesvirus (KSHV) encodes four viral interferon regulatory factors (vIRF-1-4). We investigated the mechanism and consequences of vIRF-2-mediated inhibition of interferon-response element signalling following type I interferon (IFN) induction. Western blot and electrophoretic mobility-shift assays identified the interferon-stimulated gene factor-3 (ISGF-3) components STAT1 and IRF-9 as the proximal targets of vIRF-2 activity. The biological significance of vIRF-2 inhibition of ISGF-3 was demonstrated by vIRF-2-mediated rescue of the replication of the IFN-sensitive virus encephalomyocarditis virus. This study provides both a mechanism and evidence for KSHV vIRF-2-mediated suppression of the consequences of type 1 IFN-induced signaling.",

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AU - Mutocheluh, M.

AU - Hindle, L.

AU - Aresté, C.

AU - Chanas, S. A.

AU - Butler, L. M.

AU - Lowry, K.

AU - Shah, K.

AU - Evans, D. J.

AU - Blackbourn, D. J.

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AB - Kaposi's sarcoma-associated herpesvirus (KSHV) encodes four viral interferon regulatory factors (vIRF-1-4). We investigated the mechanism and consequences of vIRF-2-mediated inhibition of interferon-response element signalling following type I interferon (IFN) induction. Western blot and electrophoretic mobility-shift assays identified the interferon-stimulated gene factor-3 (ISGF-3) components STAT1 and IRF-9 as the proximal targets of vIRF-2 activity. The biological significance of vIRF-2 inhibition of ISGF-3 was demonstrated by vIRF-2-mediated rescue of the replication of the IFN-sensitive virus encephalomyocarditis virus. This study provides both a mechanism and evidence for KSHV vIRF-2-mediated suppression of the consequences of type 1 IFN-induced signaling.

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Kaposi's sarcoma-associated herpesvirus viral interferon regulatory factor-2 inhibits type 1 interferon signalling by targeting interferon-stimulated gene factor-3

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