Lipidomics Profiling of Human Adipose Tissue Identifies a Pattern of Lipids Associated with Fish Oil Supplementation

Elizabeth G Stanley; Benjamin J Jenkins; Celia G Walker; Albert Koulman; Lucy Browning; Annette L West; Philip C Calder; Susan A Jebb; Julian L Griffin

doi:10.1021/acs.jproteome.7b00161

Lipidomics Profiling of Human Adipose Tissue Identifies a Pattern of Lipids Associated with Fish Oil Supplementation

Elizabeth G Stanley, Benjamin J Jenkins, Celia G Walker, Albert Koulman, Lucy Browning, Annette L West, Philip C Calder, Susan A Jebb, Julian L Griffin

The Rowett Institute of Nutrition and Health

University of Southampton

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

To understand the interaction between diet and health, biomarkers that accurately reflect consumption of foods of perceived health relevance are needed. The aim of this investigation was to use direct infusion-mass spectrometry (DI-MS) lipidomics to determine the effects of fish oil supplementation on lipid profiles of human adipose tissue. Adipose tissue samples from an n-3 polyunsaturated fatty acid (PUFA) supplementation study (n = 66) were analyzed to compare the pattern following supplementation equivalent to zero or four portions of oily fish per week. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were incorporated into highly unsaturated (≥5 double bonds) triglycerides (TGs), phosphocholines, and phosphoethanolamines as well as being detected directly as the nonesterified fatty acid forms. Multivariate statistics demonstrated that phospholipids were the most accurate and sensitive lipids for the assessing EPA and DHA incorporation into adipose tissue. Potential confounding factors (adiposity, age, and sex of the subject) were also considered in the analysis, and adiposity was also associated with an increase in highly unsaturated TGs as a result of incorporation of the n-6 PUFA arachidonic acid. DI-MS provides a high-throughput analysis of fatty acid status that can monitor oily fish consumption, suitable for use in cohort studies.

Original language	English
Pages (from-to)	3168-3179
Number of pages	12
Journal	Journal of Proteome Research
Volume	16
Issue number	9
Early online date	6 Jun 2017
DOIs	https://doi.org/10.1021/acs.jproteome.7b00161
Publication status	Published - 1 Sept 2017

Bibliographical note

We thank the Medical Research Council, U.K. (MC_UP_A90_1006), BBSRC (BB/H013539/2; bb/I000933/I), and the British Heart Foundation for their support. The original trial was funded by the U.K. Foods Standards Agency (N05065/66). Equazen (Wallingford, Oxford, U.K.) supplied the capsules for the intervention. The views expressed are those of the authors and do not necessarily reflect U.K. Government policy or advice.

Keywords

Adipose Tissue/chemistry
Adult
Animals
Body Weight
Case-Control Studies
Dietary Supplements
Docosahexaenoic Acids/metabolism
Eicosapentaenoic Acid/metabolism
Fatty Acids, Nonesterified/metabolism
Fatty Acids, Omega-6/metabolism
Female
Fish Oils/administration & dosage
Humans
Lipid Metabolism/drug effects
Male
Middle Aged
Phospholipids/metabolism
Principal Component Analysis
Triglycerides/metabolism

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acs.jproteome.7b00161

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title = "Lipidomics Profiling of Human Adipose Tissue Identifies a Pattern of Lipids Associated with Fish Oil Supplementation",

abstract = "To understand the interaction between diet and health, biomarkers that accurately reflect consumption of foods of perceived health relevance are needed. The aim of this investigation was to use direct infusion-mass spectrometry (DI-MS) lipidomics to determine the effects of fish oil supplementation on lipid profiles of human adipose tissue. Adipose tissue samples from an n-3 polyunsaturated fatty acid (PUFA) supplementation study (n = 66) were analyzed to compare the pattern following supplementation equivalent to zero or four portions of oily fish per week. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were incorporated into highly unsaturated (≥5 double bonds) triglycerides (TGs), phosphocholines, and phosphoethanolamines as well as being detected directly as the nonesterified fatty acid forms. Multivariate statistics demonstrated that phospholipids were the most accurate and sensitive lipids for the assessing EPA and DHA incorporation into adipose tissue. Potential confounding factors (adiposity, age, and sex of the subject) were also considered in the analysis, and adiposity was also associated with an increase in highly unsaturated TGs as a result of incorporation of the n-6 PUFA arachidonic acid. DI-MS provides a high-throughput analysis of fatty acid status that can monitor oily fish consumption, suitable for use in cohort studies.",

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author = "Stanley, {Elizabeth G} and Jenkins, {Benjamin J} and Walker, {Celia G} and Albert Koulman and Lucy Browning and West, {Annette L} and Calder, {Philip C} and Jebb, {Susan A} and Griffin, {Julian L}",

note = "We thank the Medical Research Council, U.K. (MC_UP_A90_1006), BBSRC (BB/H013539/2; bb/I000933/I), and the British Heart Foundation for their support. The original trial was funded by the U.K. Foods Standards Agency (N05065/66). Equazen (Wallingford, Oxford, U.K.) supplied the capsules for the intervention. The views expressed are those of the authors and do not necessarily reflect U.K. Government policy or advice.",

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month = sep,

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doi = "10.1021/acs.jproteome.7b00161",

language = "English",

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publisher = "American Chemical Society",

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T1 - Lipidomics Profiling of Human Adipose Tissue Identifies a Pattern of Lipids Associated with Fish Oil Supplementation

AU - Stanley, Elizabeth G

AU - Jenkins, Benjamin J

AU - Walker, Celia G

AU - Koulman, Albert

AU - Browning, Lucy

AU - West, Annette L

AU - Calder, Philip C

AU - Jebb, Susan A

AU - Griffin, Julian L

N1 - We thank the Medical Research Council, U.K. (MC_UP_A90_1006), BBSRC (BB/H013539/2; bb/I000933/I), and the British Heart Foundation for their support. The original trial was funded by the U.K. Foods Standards Agency (N05065/66). Equazen (Wallingford, Oxford, U.K.) supplied the capsules for the intervention. The views expressed are those of the authors and do not necessarily reflect U.K. Government policy or advice.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - To understand the interaction between diet and health, biomarkers that accurately reflect consumption of foods of perceived health relevance are needed. The aim of this investigation was to use direct infusion-mass spectrometry (DI-MS) lipidomics to determine the effects of fish oil supplementation on lipid profiles of human adipose tissue. Adipose tissue samples from an n-3 polyunsaturated fatty acid (PUFA) supplementation study (n = 66) were analyzed to compare the pattern following supplementation equivalent to zero or four portions of oily fish per week. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were incorporated into highly unsaturated (≥5 double bonds) triglycerides (TGs), phosphocholines, and phosphoethanolamines as well as being detected directly as the nonesterified fatty acid forms. Multivariate statistics demonstrated that phospholipids were the most accurate and sensitive lipids for the assessing EPA and DHA incorporation into adipose tissue. Potential confounding factors (adiposity, age, and sex of the subject) were also considered in the analysis, and adiposity was also associated with an increase in highly unsaturated TGs as a result of incorporation of the n-6 PUFA arachidonic acid. DI-MS provides a high-throughput analysis of fatty acid status that can monitor oily fish consumption, suitable for use in cohort studies.

AB - To understand the interaction between diet and health, biomarkers that accurately reflect consumption of foods of perceived health relevance are needed. The aim of this investigation was to use direct infusion-mass spectrometry (DI-MS) lipidomics to determine the effects of fish oil supplementation on lipid profiles of human adipose tissue. Adipose tissue samples from an n-3 polyunsaturated fatty acid (PUFA) supplementation study (n = 66) were analyzed to compare the pattern following supplementation equivalent to zero or four portions of oily fish per week. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were incorporated into highly unsaturated (≥5 double bonds) triglycerides (TGs), phosphocholines, and phosphoethanolamines as well as being detected directly as the nonesterified fatty acid forms. Multivariate statistics demonstrated that phospholipids were the most accurate and sensitive lipids for the assessing EPA and DHA incorporation into adipose tissue. Potential confounding factors (adiposity, age, and sex of the subject) were also considered in the analysis, and adiposity was also associated with an increase in highly unsaturated TGs as a result of incorporation of the n-6 PUFA arachidonic acid. DI-MS provides a high-throughput analysis of fatty acid status that can monitor oily fish consumption, suitable for use in cohort studies.

KW - Adipose Tissue/chemistry

KW - Adult

KW - Animals

KW - Body Weight

KW - Case-Control Studies

KW - Dietary Supplements

KW - Docosahexaenoic Acids/metabolism

KW - Eicosapentaenoic Acid/metabolism

KW - Fatty Acids, Nonesterified/metabolism

KW - Fatty Acids, Omega-6/metabolism

KW - Female

KW - Fish Oils/administration & dosage

KW - Humans

KW - Lipid Metabolism/drug effects

KW - Male

KW - Middle Aged

KW - Phospholipids/metabolism

KW - Principal Component Analysis

KW - Triglycerides/metabolism

U2 - 10.1021/acs.jproteome.7b00161

DO - 10.1021/acs.jproteome.7b00161

M3 - Article

C2 - 28587463

SN - 1535-3893

VL - 16

SP - 3168

EP - 3179

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 9

ER -

Lipidomics Profiling of Human Adipose Tissue Identifies a Pattern of Lipids Associated with Fish Oil Supplementation

Abstract

Bibliographical note

Keywords

UN SDGs

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