Long-term survival of Escherichia coli O157 on pasture following an outbreak associated with sheep at a scout camp

Iain D Ogden; Nichola Faye Hepburn; Norval James Colin Strachan; Thomas Hugh Pennington; Marion MacRae; D. R. Fenlon; S. M. Rusbridge

doi:10.1046/j.1472-765x.2002.01052.x

Long-term survival of Escherichia coli O157 on pasture following an outbreak associated with sheep at a scout camp

Iain D Ogden, Nichola Faye Hepburn, Norval James Colin Strachan, Thomas Hugh Pennington, Marion MacRae, D. R. Fenlon, S. M. Rusbridge

Research output: Contribution to journal › Article › peer-review

97 Citations (Scopus)

Abstract

Aims: To monitor the decay of E. coli O157 in soil (loamy sand) on a scout campsite following an outbreak in humans.

Methods and Results: Samples of soil and sheep faeces were collected from the campsite and tested for the presence of E. coli O157 by immunomagnetic separation (IMS) after enrichment in buffered peptone water + vancomycin at 42degreesC for 6 h. Enumeration of target was carried out by direct plating onto sorbitol MacConkey agar plates supplemented with cefixime and tellurite (CTSMAC) incubated at 37degreesC for 24 h. Low numbers (<100 g(-1)) were estimated by the most probable number (3-tube MPN) technique.

Conclusions: Survival was observed for 15 weeks.

Significance and Impact of the Study: A number of laboratory studies have followed the decay of E. coli O157 in soil, animal faeces and water. This study follows (for the first time) the decay of the organism in soil after an outbreak associated with sheep. It demonstrates the long-term persistence of the organism in the environment and the results will be potentially important in performing risk assessments for both human and animal infection.

Original language	English
Pages (from-to)	100-104
Number of pages	4
Journal	Letters in Applied Microbiology
Volume	34
Issue number	2
DOIs	https://doi.org/10.1046/j.1472-765x.2002.01052.x
Publication status	Published - 2002

Keywords

HEMOLYTIC-UREMIC SYNDROME
STRAINS

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title = "Long-term survival of Escherichia coli O157 on pasture following an outbreak associated with sheep at a scout camp",

abstract = "Aims: To monitor the decay of E. coli O157 in soil (loamy sand) on a scout campsite following an outbreak in humans.Methods and Results: Samples of soil and sheep faeces were collected from the campsite and tested for the presence of E. coli O157 by immunomagnetic separation (IMS) after enrichment in buffered peptone water + vancomycin at 42degreesC for 6 h. Enumeration of target was carried out by direct plating onto sorbitol MacConkey agar plates supplemented with cefixime and tellurite (CTSMAC) incubated at 37degreesC for 24 h. Low numbers (<100 g(-1)) were estimated by the most probable number (3-tube MPN) technique.Conclusions: Survival was observed for 15 weeks.Significance and Impact of the Study: A number of laboratory studies have followed the decay of E. coli O157 in soil, animal faeces and water. This study follows (for the first time) the decay of the organism in soil after an outbreak associated with sheep. It demonstrates the long-term persistence of the organism in the environment and the results will be potentially important in performing risk assessments for both human and animal infection.",

keywords = "HEMOLYTIC-UREMIC SYNDROME, STRAINS",

author = "Ogden, {Iain D} and Hepburn, {Nichola Faye} and Strachan, {Norval James Colin} and Pennington, {Thomas Hugh} and Marion MacRae and Fenlon, {D. R.} and Rusbridge, {S. M.}",

year = "2002",

doi = "10.1046/j.1472-765x.2002.01052.x",

language = "English",

volume = "34",

pages = "100--104",

journal = "Letters in Applied Microbiology",

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T1 - Long-term survival of Escherichia coli O157 on pasture following an outbreak associated with sheep at a scout camp

AU - Ogden, Iain D

AU - Hepburn, Nichola Faye

AU - Strachan, Norval James Colin

AU - Pennington, Thomas Hugh

AU - MacRae, Marion

AU - Fenlon, D. R.

AU - Rusbridge, S. M.

PY - 2002

Y1 - 2002

N2 - Aims: To monitor the decay of E. coli O157 in soil (loamy sand) on a scout campsite following an outbreak in humans.Methods and Results: Samples of soil and sheep faeces were collected from the campsite and tested for the presence of E. coli O157 by immunomagnetic separation (IMS) after enrichment in buffered peptone water + vancomycin at 42degreesC for 6 h. Enumeration of target was carried out by direct plating onto sorbitol MacConkey agar plates supplemented with cefixime and tellurite (CTSMAC) incubated at 37degreesC for 24 h. Low numbers (<100 g(-1)) were estimated by the most probable number (3-tube MPN) technique.Conclusions: Survival was observed for 15 weeks.Significance and Impact of the Study: A number of laboratory studies have followed the decay of E. coli O157 in soil, animal faeces and water. This study follows (for the first time) the decay of the organism in soil after an outbreak associated with sheep. It demonstrates the long-term persistence of the organism in the environment and the results will be potentially important in performing risk assessments for both human and animal infection.

AB - Aims: To monitor the decay of E. coli O157 in soil (loamy sand) on a scout campsite following an outbreak in humans.Methods and Results: Samples of soil and sheep faeces were collected from the campsite and tested for the presence of E. coli O157 by immunomagnetic separation (IMS) after enrichment in buffered peptone water + vancomycin at 42degreesC for 6 h. Enumeration of target was carried out by direct plating onto sorbitol MacConkey agar plates supplemented with cefixime and tellurite (CTSMAC) incubated at 37degreesC for 24 h. Low numbers (<100 g(-1)) were estimated by the most probable number (3-tube MPN) technique.Conclusions: Survival was observed for 15 weeks.Significance and Impact of the Study: A number of laboratory studies have followed the decay of E. coli O157 in soil, animal faeces and water. This study follows (for the first time) the decay of the organism in soil after an outbreak associated with sheep. It demonstrates the long-term persistence of the organism in the environment and the results will be potentially important in performing risk assessments for both human and animal infection.

KW - HEMOLYTIC-UREMIC SYNDROME

KW - STRAINS

U2 - 10.1046/j.1472-765x.2002.01052.x

DO - 10.1046/j.1472-765x.2002.01052.x

M3 - Article

SN - 0266-8254

VL - 34

SP - 100

EP - 104

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 2

ER -

Long-term survival of Escherichia coli O157 on pasture following an outbreak associated with sheep at a scout camp

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Keywords

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