Abstract
It has been proposed that in utero factors may predispose towards the development of childhood atopy. To test this hypothesis, it will be necessary to measure T-helper cell (Th) cytokines secreted by human cord blood mononuclear cells (CBMC) stimulated by allergens. However, to date, it has proven impossible to measure allergen-specific CBMC secretion of the key Th cytokine interleukin-4 (IL-4) using conventional sandwich ELISA techniques. We report for the first time the successful measurement of IL-4 secreted by CBMC stimulated by the allergens timothy grass pollen and house dust mite extract. The method is an adaptation of a novel cell-based ELISA (celELISA), which demonstrated an increased (up to 20-fold) sensitivity to detect IL-4. The method is simple, precise, is no more costly than a conventional ELISA, and can identify individuals in a general population whose CBMC exhibit different cytokine biases in response to allergens. The frequency distribution of IL-4 and interferon-gamma (IFN-gamma) CBMC responses to allergens in the general population approximates to a log-normal distribution, which will permit the application of linear regression techniques in the identification of in utero factors which influence Th bias.
Original language | English |
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Pages (from-to) | 13-22 |
Number of pages | 10 |
Journal | Journal of Immunological Methods |
Volume | 234 |
Issue number | 1-2 |
DOIs | |
Publication status | Published - 3 Feb 2000 |
Keywords
- Allergens
- Animals
- Cell Culture Techniques
- Cells, Cultured
- Enzyme-Linked Immunosorbent Assay
- Female
- Fetal Blood
- Humans
- Interferon-gamma
- Interleukin-4
- Leukocytes, Mononuclear
- Mites
- Poaceae
- Pollen
- Pregnancy
- Reproducibility of Results
- Sensitivity and Specificity
- T-Lymphocytes, Helper-Inducer
- Interferon-¿
- T-helper subsets
- Human cord blood mononuclear cells
- ELISA
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Maternal allergen exposure during pregnancy and childhood allergy: changing UK Public Health policy
Anthony Seaton (Coordinator) & Graham Devereux (Coordinator)
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