Metabolomics profiling reveals the detoxification and tolerance behavior of two bread wheat (Triticum aestivum L.) varieties under arsenate stress

Muhammad Saeed; Umar Masood Quraishi; Ghazala Mustafa; Abida Farooqi; Maria Greger; Riffat Naseem Malik

doi:10.1016/j.foodchem.2024.138612

Metabolomics profiling reveals the detoxification and tolerance behavior of two bread wheat (Triticum aestivum L.) varieties under arsenate stress

Muhammad Saeed, Umar Masood Quraishi, Ghazala Mustafa, Abida Farooqi, Maria Greger, Riffat Naseem Malik^* (Corresponding Author)

^*Corresponding author for this work

Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

The present study conducted metabolomics profiling (targeted and untargeted) in the roots of two wheat varieties (BARANI-70 and NARC-09) under arsenate stress in a hydroponic experiment. The findings indicated a better growth response of BARANI-70 compared to the NARC-09. From amino acid profiling, a total of 26 amino acids (AAs) were quantified in roots. BARANI-70 showed higher induction of stress-responsive AAs compared to the NARC-09. From untargeted metabolomics, a total of 136 metabolites were identified: AAs, fatty acids, purines, carnitines, LysoPCs, and others. The KEGG pathway identified pathways such as linoleic acid metabolism, TCA cycle, glutathione metabolism, and aminoacyl-tRNA biosynthesis that were regulated to improve the defense of tolerant variety. BARANI-70 emerged as a tolerant variety based on the psychological response, As accumulation, and behavior of stress-responsive metabolites. This study should facilitate the breeding of low-As accumulating wheat varieties for future application to ensure sustainable production and food safety.

Original language	English
Article number	138612
Number of pages	15
Journal	Food Chemistry
Volume	443
Early online date	1 Feb 2024
DOIs	https://doi.org/10.1016/j.foodchem.2024.138612
Publication status	E-pub ahead of print - 1 Feb 2024

Bibliographical note

Acknowledgment
Swedish Metabolomics Centre, Umeå, Sweden (www.swedishmetabolomicscentre.se) is acknowledged for metabolic profiling by LC-TOF-MS and amino acid quantification by LC-QqQ-MSMS. We acknowledge Muhammad Anas (Quaid-i-Azam University, Islamabad) for his valuable contribution for data analysis in this study.

Data Availability Statement

Data will be made available on request.

Keywords

Amino acids
Arsenate reductase
Tolerance
Omics
Glutathione

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title = "Metabolomics profiling reveals the detoxification and tolerance behavior of two bread wheat (Triticum aestivum L.) varieties under arsenate stress",

abstract = "The present study conducted metabolomics profiling (targeted and untargeted) in the roots of two wheat varieties (BARANI-70 and NARC-09) under arsenate stress in a hydroponic experiment. The findings indicated a better growth response of BARANI-70 compared to the NARC-09. From amino acid profiling, a total of 26 amino acids (AAs) were quantified in roots. BARANI-70 showed higher induction of stress-responsive AAs compared to the NARC-09. From untargeted metabolomics, a total of 136 metabolites were identified: AAs, fatty acids, purines, carnitines, LysoPCs, and others. The KEGG pathway identified pathways such as linoleic acid metabolism, TCA cycle, glutathione metabolism, and aminoacyl-tRNA biosynthesis that were regulated to improve the defense of tolerant variety. BARANI-70 emerged as a tolerant variety based on the psychological response, As accumulation, and behavior of stress-responsive metabolites. This study should facilitate the breeding of low-As accumulating wheat varieties for future application to ensure sustainable production and food safety.",

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note = "Acknowledgment Swedish Metabolomics Centre, Ume{\aa}, Sweden (www.swedishmetabolomicscentre.se) is acknowledged for metabolic profiling by LC-TOF-MS and amino acid quantification by LC-QqQ-MSMS. We acknowledge Muhammad Anas (Quaid-i-Azam University, Islamabad) for his valuable contribution for data analysis in this study.",

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AU - Farooqi, Abida

AU - Greger, Maria

AU - Naseem Malik, Riffat

N1 - Acknowledgment Swedish Metabolomics Centre, Umeå, Sweden (www.swedishmetabolomicscentre.se) is acknowledged for metabolic profiling by LC-TOF-MS and amino acid quantification by LC-QqQ-MSMS. We acknowledge Muhammad Anas (Quaid-i-Azam University, Islamabad) for his valuable contribution for data analysis in this study.

PY - 2024/2/1

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N2 - The present study conducted metabolomics profiling (targeted and untargeted) in the roots of two wheat varieties (BARANI-70 and NARC-09) under arsenate stress in a hydroponic experiment. The findings indicated a better growth response of BARANI-70 compared to the NARC-09. From amino acid profiling, a total of 26 amino acids (AAs) were quantified in roots. BARANI-70 showed higher induction of stress-responsive AAs compared to the NARC-09. From untargeted metabolomics, a total of 136 metabolites were identified: AAs, fatty acids, purines, carnitines, LysoPCs, and others. The KEGG pathway identified pathways such as linoleic acid metabolism, TCA cycle, glutathione metabolism, and aminoacyl-tRNA biosynthesis that were regulated to improve the defense of tolerant variety. BARANI-70 emerged as a tolerant variety based on the psychological response, As accumulation, and behavior of stress-responsive metabolites. This study should facilitate the breeding of low-As accumulating wheat varieties for future application to ensure sustainable production and food safety.

AB - The present study conducted metabolomics profiling (targeted and untargeted) in the roots of two wheat varieties (BARANI-70 and NARC-09) under arsenate stress in a hydroponic experiment. The findings indicated a better growth response of BARANI-70 compared to the NARC-09. From amino acid profiling, a total of 26 amino acids (AAs) were quantified in roots. BARANI-70 showed higher induction of stress-responsive AAs compared to the NARC-09. From untargeted metabolomics, a total of 136 metabolites were identified: AAs, fatty acids, purines, carnitines, LysoPCs, and others. The KEGG pathway identified pathways such as linoleic acid metabolism, TCA cycle, glutathione metabolism, and aminoacyl-tRNA biosynthesis that were regulated to improve the defense of tolerant variety. BARANI-70 emerged as a tolerant variety based on the psychological response, As accumulation, and behavior of stress-responsive metabolites. This study should facilitate the breeding of low-As accumulating wheat varieties for future application to ensure sustainable production and food safety.

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Metabolomics profiling reveals the detoxification and tolerance behavior of two bread wheat (Triticum aestivum L.) varieties under arsenate stress

Abstract

Bibliographical note

Data Availability Statement

Keywords

Access to Document

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