Microbiota that affect risk for shigellosis in children in low-income countries

Brianna Lindsay, Joe Oundo, M Anowar Hossain, Martin Antonio, Boubou Tamboura, Alan W Walker, Joseph N Paulson, Julian Parkhill, Richard Omore, Abu S G Faruque, Suman Kumar Das, Usman N Ikumapayi, Mitchell Adeyemi, Doh Sanogo, Debasish Saha, Samba Sow, Tamer H Farag, Dilruba Nasrin, Shan Li, Sandra PanchalingamMyron M Levine, Karen Kotloff, Laurence S Magder, Laura Hungerford, Halvor Sommerfelt, Mihai Pop, James P Nataro, O Colin Stine

Research output: Contribution to journalArticlepeer-review

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Pathogens in the gastrointestinal tract exist within a vast population of microbes. We examined associations between pathogens and composition of gut microbiota as they relate to Shigella spp./enteroinvasive Escherichia coli infection. We analyzed 3,035 stool specimens (1,735 nondiarrheal and 1,300 moderate-to-severe diarrheal) from the Global Enteric Multicenter Study for 9 enteropathogens. Diarrheal specimens had a higher number of enteropathogens (diarrheal mean 1.4, nondiarrheal mean 0.95; p<0.0001). Rotavirus showed a negative association with Shigella spp. in cases of diarrhea (odds ratio 0.31, 95% CI 0.17-0.55) and had a large combined effect on moderate-to-severe diarrhea (odds ratio 29, 95% CI 3.8-220). In 4 Lactobacillus taxa identified by 16S rRNA gene sequencing, the association between pathogen and disease was decreased, which is consistent with the possibility that Lactobacillus spp. are protective against Shigella spp.-induced diarrhea. Bacterial diversity of gut microbiota was associated with diarrhea status, not high levels of the Shigella spp. ipaH gene.

Original languageEnglish
Pages (from-to)242-250
Number of pages9
JournalEmerging Infectious Diseases
Issue number2
Publication statusPublished - Feb 2015

Bibliographical note

We thank Carol Tackett for her consultation on clinical terminology and readability.

B.L., J.P.N., and O.C.S. developed the hypotheses; M.M.L., K.K., and J.P.N. led the Global Enteric Multicenter Study; B.L., J.O., M.A.H., M.A., B.T., R.O., A.S.G.F., S.K.D., U.N.I., M.A., D. Sanogo, D. Saha, S.S., T.H.F., D.N., and S.P. collected data and developed and maintain databases; A.W.W., J.N.P., J.P., M.P., and O.C.S. developed and performed 16S rRNA gene sequencing, assembly, and taxonomic identification; S.L., B.L., and S.P. performed laboratory protocols; and B.L., L.S.M., L.H., and H.S. performed analyses.


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