Mitotic Golgi partitioning is driven by the membrane-fissioning protein CtBP3/BARS

Cristina Hidalgo Carcedo; Matteo Bonazzi; Stefania Spanò; Gabriele Turacchio; Antonino Colanzi; Alberto Luini; Daniela Corda

doi:10.1126/science.1097775

Mitotic Golgi partitioning is driven by the membrane-fissioning protein CtBP3/BARS

Cristina Hidalgo Carcedo, Matteo Bonazzi, Stefania Spanò, Gabriele Turacchio, Antonino Colanzi, Alberto Luini, Daniela Corda

Medical Sciences

Research output: Contribution to journal › Article › peer-review

110 Citations (Scopus)

Abstract

Organelle inheritance is an essential feature of all eukaryotic cells. As with other organelles, the Golgi complex partitions between daughter cells through the fission of its membranes into numerous tubulovesicular fragments. We found that the protein CtBP3/BARS (BARS) was responsible for driving the fission of Golgi membranes during mitosis in vivo. Moreover, by in vitro analysis, we identified two stages of this Golgi fragmentation process: disassembly of the Golgi stacks into a tubular network, and BARS-dependent fission of these tubules. Finally, this BARS-induced fission of Golgi membranes controlled the G2-to-prophase transition of the cell cycle, and hence cell division.

Original language	English
Pages (from-to)	93-6
Number of pages	4
Journal	Science
Volume	305
Issue number	5680
DOIs	https://doi.org/10.1126/science.1097775
Publication status	Published - 2 Jul 2004

Keywords

Animals
Carrier Proteins
Cell Line
Cytosol
G2 Phase
Golgi Apparatus
Interphase
Intracellular Membranes
Mitosis
Oligonucleotides, Antisense
Protein Structure, Tertiary
Rats
Recombinant Proteins
Transcription Factors

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title = "Mitotic Golgi partitioning is driven by the membrane-fissioning protein CtBP3/BARS",

abstract = "Organelle inheritance is an essential feature of all eukaryotic cells. As with other organelles, the Golgi complex partitions between daughter cells through the fission of its membranes into numerous tubulovesicular fragments. We found that the protein CtBP3/BARS (BARS) was responsible for driving the fission of Golgi membranes during mitosis in vivo. Moreover, by in vitro analysis, we identified two stages of this Golgi fragmentation process: disassembly of the Golgi stacks into a tubular network, and BARS-dependent fission of these tubules. Finally, this BARS-induced fission of Golgi membranes controlled the G2-to-prophase transition of the cell cycle, and hence cell division.",

keywords = "Animals, Carrier Proteins, Cell Line, Cytosol, G2 Phase, Golgi Apparatus, Interphase, Intracellular Membranes, Mitosis, Oligonucleotides, Antisense, Protein Structure, Tertiary, Rats, Recombinant Proteins, Transcription Factors",

author = "{Hidalgo Carcedo}, Cristina and Matteo Bonazzi and Stefania Span{\`o} and Gabriele Turacchio and Antonino Colanzi and Alberto Luini and Daniela Corda",

year = "2004",

month = jul,

day = "2",

doi = "10.1126/science.1097775",

language = "English",

volume = "305",

pages = "93--6",

journal = "Science",

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TY - JOUR

T1 - Mitotic Golgi partitioning is driven by the membrane-fissioning protein CtBP3/BARS

AU - Hidalgo Carcedo, Cristina

AU - Bonazzi, Matteo

AU - Spanò, Stefania

AU - Turacchio, Gabriele

AU - Colanzi, Antonino

AU - Luini, Alberto

AU - Corda, Daniela

PY - 2004/7/2

Y1 - 2004/7/2

N2 - Organelle inheritance is an essential feature of all eukaryotic cells. As with other organelles, the Golgi complex partitions between daughter cells through the fission of its membranes into numerous tubulovesicular fragments. We found that the protein CtBP3/BARS (BARS) was responsible for driving the fission of Golgi membranes during mitosis in vivo. Moreover, by in vitro analysis, we identified two stages of this Golgi fragmentation process: disassembly of the Golgi stacks into a tubular network, and BARS-dependent fission of these tubules. Finally, this BARS-induced fission of Golgi membranes controlled the G2-to-prophase transition of the cell cycle, and hence cell division.

AB - Organelle inheritance is an essential feature of all eukaryotic cells. As with other organelles, the Golgi complex partitions between daughter cells through the fission of its membranes into numerous tubulovesicular fragments. We found that the protein CtBP3/BARS (BARS) was responsible for driving the fission of Golgi membranes during mitosis in vivo. Moreover, by in vitro analysis, we identified two stages of this Golgi fragmentation process: disassembly of the Golgi stacks into a tubular network, and BARS-dependent fission of these tubules. Finally, this BARS-induced fission of Golgi membranes controlled the G2-to-prophase transition of the cell cycle, and hence cell division.

KW - Animals

KW - Carrier Proteins

KW - Cell Line

KW - Cytosol

KW - G2 Phase

KW - Golgi Apparatus

KW - Interphase

KW - Intracellular Membranes

KW - Mitosis

KW - Oligonucleotides, Antisense

KW - Protein Structure, Tertiary

KW - Rats

KW - Recombinant Proteins

KW - Transcription Factors

U2 - 10.1126/science.1097775

DO - 10.1126/science.1097775

M3 - Article

C2 - 15232108

SN - 0036-8075

VL - 305

SP - 93

EP - 96

JO - Science

JF - Science

IS - 5680

ER -

Mitotic Golgi partitioning is driven by the membrane-fissioning protein CtBP3/BARS

Abstract

Keywords

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