Mn-Loaded apolactoferrin dots for in vivo MRI and NIR-II cancer imaging

Hui Zhou, Han Yang, Lin Tang, Yue Wang, Yang Li, Nan Liu, Xiaodong Zeng, Yu Yan, Junzhu Wu, Shizhen Chen, Long Xiao, Yi Yu, Zixin Deng, Hai Deng, Xuechuan Hong (Corresponding Author), Yuling Xiao (Corresponding Author)

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

The integration of molecular imaging probes into a biodegradable, noncytotoxic and biocompatible nanoplatform is very promising for high efficacy tumor diagnosis but is still a great challenge. Herein, lactoferrin (Lf), an iron-binding glycoprotein, was applied to develop a novel molecular platform. Biocompatible Mn-loaded apolactoferrin Mn2+-Apo-Lf-PEG was first prepared. The first dual-modality imaging NIR-II/MRI nanoprobe (H-dot) was then constructed by linking a NIR-II fluorescent probe (CH1055) to the surface of Mn2+-Apo-Lf-PEG via glutaraldehyde cross linking. Cellular studies showed that the obtained H-dot exhibited low toxicity, excellent stability, biocompatibility, significantly enhanced T1-weighted magnetic resonance imaging (MRI) and superior NIR-II imaging optical properties. The H-dot was subsequently evaluated in subcutaneous HepG2 liver xenografts and subcutaneous/orthotopic U87MG glioblastoma xenografts, revealing its excellent dual imaging properties in small-animal models for the first time, such as high tumor specificity and contrast, good tumor uptake, and accurate tumor delineation. These particular properties of the Mn-loaded apolactoferrin dotmake it a promisingmolecular platform for the development of novel imaging probes and clinical translation.

Original languageEnglish
Pages (from-to)9448-9454
Number of pages7
JournalJournal of Materials Chemistry A
Volume7
Issue number31
Early online date11 Jul 2019
DOIs
Publication statusPublished - 21 Aug 2019

Bibliographical note

Acknowledgements
This work was partially supported by grants from the National Natural Science Foundation of China (81773674, 81573383 and 21763002), the NSFHP (2017CFA024, 2017CFB711, and 2016ACA126), the Health Commission of Hubei Province Scientific Research Project (WJ2019M177 and WJ2019M178), the Tibet Autonomous Region Science and Technology Plan Project Key Project (XZ201901-GB-11), the Shenzhen Science and Technology Research Grant (JCYJ20170303170809222) and the Fundamental Research Funds for the Central Universities.

Keywords

  • CONTRAST AGENT
  • NANOPARTICLES
  • FLUORESCENCE
  • MANGANESE
  • FLUOROPHORES
  • WAVELENGTHS
  • LACTOFERRIN
  • COMPLEXES
  • CHROMIUM
  • COBALT

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