Abstract
The integration of molecular imaging probes into a biodegradable, noncytotoxic and biocompatible nanoplatform is very promising for high efficacy tumor diagnosis but is still a great challenge. Herein, lactoferrin (Lf), an iron-binding glycoprotein, was applied to develop a novel molecular platform. Biocompatible Mn-loaded apolactoferrin Mn2+-Apo-Lf-PEG was first prepared. The first dual-modality imaging NIR-II/MRI nanoprobe (H-dot) was then constructed by linking a NIR-II fluorescent probe (CH1055) to the surface of Mn2+-Apo-Lf-PEG via glutaraldehyde cross linking. Cellular studies showed that the obtained H-dot exhibited low toxicity, excellent stability, biocompatibility, significantly enhanced T1-weighted magnetic resonance imaging (MRI) and superior NIR-II imaging optical properties. The H-dot was subsequently evaluated in subcutaneous HepG2 liver xenografts and subcutaneous/orthotopic U87MG glioblastoma xenografts, revealing its excellent dual imaging properties in small-animal models for the first time, such as high tumor specificity and contrast, good tumor uptake, and accurate tumor delineation. These particular properties of the Mn-loaded apolactoferrin dotmake it a promisingmolecular platform for the development of novel imaging probes and clinical translation.
Original language | English |
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Pages (from-to) | 9448-9454 |
Number of pages | 7 |
Journal | Journal of Materials Chemistry A |
Volume | 7 |
Issue number | 31 |
Early online date | 11 Jul 2019 |
DOIs | |
Publication status | Published - 21 Aug 2019 |
Bibliographical note
AcknowledgementsThis work was partially supported by grants from the National Natural Science Foundation of China (81773674, 81573383 and 21763002), the NSFHP (2017CFA024, 2017CFB711, and 2016ACA126), the Health Commission of Hubei Province Scientific Research Project (WJ2019M177 and WJ2019M178), the Tibet Autonomous Region Science and Technology Plan Project Key Project (XZ201901-GB-11), the Shenzhen Science and Technology Research Grant (JCYJ20170303170809222) and the Fundamental Research Funds for the Central Universities.
Keywords
- CONTRAST AGENT
- NANOPARTICLES
- FLUORESCENCE
- MANGANESE
- FLUOROPHORES
- WAVELENGTHS
- LACTOFERRIN
- COMPLEXES
- CHROMIUM
- COBALT