Modular gene over-expression strategies for Candida albicans

Vitor  Cabral; Murielle  Chauvel; Arnaud Firon; Audrey Nesseir; Sophie Bachellier-Bassi; Yogesh  Chaudhari; Carol Anne Munro; Christophe d'Enfert

doi:10.1007/978-1-61779-539-8_15

Modular gene over-expression strategies for Candida albicans

Vitor Cabral, Murielle Chauvel, Arnaud Firon, Audrey Nesseir, Sophie Bachellier-Bassi, Yogesh Chaudhari, Carol Anne Munro, Christophe d'Enfert

Institut Pasteur Paris

Research output: Chapter in Book/Report/Conference proceeding › Chapter

15 Citations (Scopus)

Abstract

Over-expression is a valid functional genomics approach to characterise genes of unknown function on a genome-wide scale. Strains are engineered to over-express a specific gene and the resulting gain-of-function phenotype assessed. Here, we describe the strategy we are adopting to synthesise a Candida albicans ORFeome collection and the options available to create over-expressing strains from this collection.

Original language	English
Title of host publication	Host-Fungus Interactions
Subtitle of host publication	Methods and Protocols
Editors	Alexandra Brand, Donna MacCallum
Place of Publication	NewYork
Publisher	Humana Press
Pages	227-244
Number of pages	18
Volume	2
ISBN (Print)	9781617795381
DOIs	https://doi.org/10.1007/978-1-61779-539-8_15
Publication status	Published - 29 Feb 2012

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	845
ISSN (Print)	1064-3745

Keywords

Gateway cloning
Genetic transformation
Integrative vectors
Over-expression
Strain storage
TAP tagging
Western blotting

Access to Document

10.1007/978-1-61779-539-8_15

Cite this

Cabral, V., Chauvel, M., Firon, A., Nesseir, A., Bachellier-Bassi, S., Chaudhari, Y., Munro, C. A., & d'Enfert, C. (2012). Modular gene over-expression strategies for Candida albicans. In A. Brand, & D. MacCallum (Eds.), Host-Fungus Interactions: Methods and Protocols (Vol. 2, pp. 227-244). (Methods in Molecular Biology; Vol. 845). Humana Press. https://doi.org/10.1007/978-1-61779-539-8_15

Modular gene over-expression strategies for Candida albicans. / Cabral, Vitor ; Chauvel, Murielle ; Firon, Arnaud et al.
Host-Fungus Interactions: Methods and Protocols. ed. / Alexandra Brand; Donna MacCallum. Vol. 2 NewYork: Humana Press, 2012. p. 227-244 (Methods in Molecular Biology; Vol. 845).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Cabral, V, Chauvel, M, Firon, A, Nesseir, A, Bachellier-Bassi, S, Chaudhari, Y, Munro, CA & d'Enfert, C 2012, Modular gene over-expression strategies for Candida albicans. in A Brand & D MacCallum (eds), Host-Fungus Interactions: Methods and Protocols. vol. 2, Methods in Molecular Biology, vol. 845, Humana Press, NewYork, pp. 227-244. https://doi.org/10.1007/978-1-61779-539-8_15

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Modular gene over-expression strategies for Candida albicans

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