The human gut microbiota plays a major role in the development of colorectal cancer (CRC). Many studies have attempted to define links between microbiota residents, their function and disease development. We now have incredible molecular tools to allow us to study the gut microbiome however in order to make best use of these sophisticated approaches we need to ensure that samples are collected and processed using standardized and reproducible protocols. Here we provide an overview of molecular analysis methods and describe protocols for collecting and processing clinical samples for subsequent microbiome analysis.
|Title of host publication||Colorectal Cancer|
|Subtitle of host publication||Methods and Protocols|
|Number of pages||15|
|Publication status||Published - 27 Mar 2018|
|Name||Methods in Molecular Biology|
Bibliographical noteThis work was supported by grants from The Chief Scientist Office, Friends of Anchor, NHS Grampian Endowments, NHS Grampian Gastroenterology Endowment funds as well as donations from CRC patients.
- Journal Article
- Colorectal cancer
- Microbial diversity
- Clinical samples
- Molecular microbiology