Animal colonization and infection models are frequently used to investigate host-pathogen interactions and disease progression. Here, we describe an effective model to investigate the ability of the newly emerged fungal pathogen Candida auris to persistently colonize the gut of immunocompetent mice. In our model, mice are inoculated by gavage and are subsequently monitored for colonization by determining daily fungal stool burdens. At the end of the experiment, mice are culled, and their spleen, liver, kidneys, lungs, heart, and caecum harvested to determine the fungal burdens in order to investigate colonization and potentially dissemination of C. auris to other host organs.
We thank the University of Aberdeen Medical Research Facility staff, as well as Dr. Ambre F. Chapuis, for their assistance with the mouse experiments. We also thank the A. G. Leventis Foundation for supporting Mr. Stylianos C. Simantirakis during his PhD studies. Dr. Donna MacCallum’s research was supported by BBSRC (BB/P02050X/1), European Commission (847507 HDM-FUN), and NC3Rs (NC/S001557/1 and NC/N002482/1).
- Antifungal Agents
- Candida auris
- Disease Models, Animal
- Host-Pathogen Interactions