Mouse Gastrointestinal Colonization Model for Candida auris

Stylianos C Simantirakis; Donna M MacCallum

doi:10.1007/978-1-0716-2417-3_26

Mouse Gastrointestinal Colonization Model for Candida auris

Stylianos C Simantirakis, Donna M MacCallum^* (Corresponding Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Animal colonization and infection models are frequently used to investigate host-pathogen interactions and disease progression. Here, we describe an effective model to investigate the ability of the newly emerged fungal pathogen Candida auris to persistently colonize the gut of immunocompetent mice. In our model, mice are inoculated by gavage and are subsequently monitored for colonization by determining daily fungal stool burdens. At the end of the experiment, mice are culled, and their spleen, liver, kidneys, lungs, heart, and caecum harvested to determine the fungal burdens in order to investigate colonization and potentially dissemination of C. auris to other host organs.

Original language	English
Pages (from-to)	329-340
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2517
DOIs	https://doi.org/10.1007/978-1-0716-2417-3_26
Publication status	Published - 9 Jun 2022

Bibliographical note

Acknowledgements
We thank the University of Aberdeen Medical Research Facility staff, as well as Dr. Ambre F. Chapuis, for their assistance with the mouse experiments. We also thank the A. G. Leventis Foundation for supporting Mr. Stylianos C. Simantirakis during his PhD studies. Dr. Donna MacCallum’s research was supported by BBSRC (BB/P02050X/1), European Commission (847507 HDM-FUN), and NC3Rs (NC/S001557/1 and NC/N002482/1).

Keywords

Animals
Antifungal Agents
Candida
Candida auris
Candidiasis/microbiology
Disease Models, Animal
Host-Pathogen Interactions
Mice

Access to Document

10.1007/978-1-0716-2417-3_26Licence: Unspecified

Cite this

@article{cdcbf7ed9f3743c0b8cc5f838fb8ad7d,

title = "Mouse Gastrointestinal Colonization Model for Candida auris",

abstract = "Animal colonization and infection models are frequently used to investigate host-pathogen interactions and disease progression. Here, we describe an effective model to investigate the ability of the newly emerged fungal pathogen Candida auris to persistently colonize the gut of immunocompetent mice. In our model, mice are inoculated by gavage and are subsequently monitored for colonization by determining daily fungal stool burdens. At the end of the experiment, mice are culled, and their spleen, liver, kidneys, lungs, heart, and caecum harvested to determine the fungal burdens in order to investigate colonization and potentially dissemination of C. auris to other host organs.",

keywords = "Animals, Antifungal Agents, Candida, Candida auris, Candidiasis/microbiology, Disease Models, Animal, Host-Pathogen Interactions, Mice",

author = "Simantirakis, {Stylianos C} and MacCallum, {Donna M}",

note = "Acknowledgements We thank the University of Aberdeen Medical Research Facility staff, as well as Dr. Ambre F. Chapuis, for their assistance with the mouse experiments. We also thank the A. G. Leventis Foundation for supporting Mr. Stylianos C. Simantirakis during his PhD studies. Dr. Donna MacCallum{\textquoteright}s research was supported by BBSRC (BB/P02050X/1), European Commission (847507 HDM-FUN), and NC3Rs (NC/S001557/1 and NC/N002482/1).",

year = "2022",

month = jun,

day = "9",

doi = "10.1007/978-1-0716-2417-3_26",

language = "English",

volume = "2517",

pages = "329--340",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Mouse Gastrointestinal Colonization Model for Candida auris

AU - Simantirakis, Stylianos C

AU - MacCallum, Donna M

N1 - Acknowledgements We thank the University of Aberdeen Medical Research Facility staff, as well as Dr. Ambre F. Chapuis, for their assistance with the mouse experiments. We also thank the A. G. Leventis Foundation for supporting Mr. Stylianos C. Simantirakis during his PhD studies. Dr. Donna MacCallum’s research was supported by BBSRC (BB/P02050X/1), European Commission (847507 HDM-FUN), and NC3Rs (NC/S001557/1 and NC/N002482/1).

PY - 2022/6/9

Y1 - 2022/6/9

N2 - Animal colonization and infection models are frequently used to investigate host-pathogen interactions and disease progression. Here, we describe an effective model to investigate the ability of the newly emerged fungal pathogen Candida auris to persistently colonize the gut of immunocompetent mice. In our model, mice are inoculated by gavage and are subsequently monitored for colonization by determining daily fungal stool burdens. At the end of the experiment, mice are culled, and their spleen, liver, kidneys, lungs, heart, and caecum harvested to determine the fungal burdens in order to investigate colonization and potentially dissemination of C. auris to other host organs.

AB - Animal colonization and infection models are frequently used to investigate host-pathogen interactions and disease progression. Here, we describe an effective model to investigate the ability of the newly emerged fungal pathogen Candida auris to persistently colonize the gut of immunocompetent mice. In our model, mice are inoculated by gavage and are subsequently monitored for colonization by determining daily fungal stool burdens. At the end of the experiment, mice are culled, and their spleen, liver, kidneys, lungs, heart, and caecum harvested to determine the fungal burdens in order to investigate colonization and potentially dissemination of C. auris to other host organs.

KW - Animals

KW - Antifungal Agents

KW - Candida

KW - Candida auris

KW - Candidiasis/microbiology

KW - Disease Models, Animal

KW - Host-Pathogen Interactions

KW - Mice

U2 - 10.1007/978-1-0716-2417-3_26

DO - 10.1007/978-1-0716-2417-3_26

M3 - Article

C2 - 35674966

SN - 1064-3745

VL - 2517

SP - 329

EP - 340

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Mouse Gastrointestinal Colonization Model for Candida auris

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this