Myostatin dysfunction is associated with reduction in overload induced hypertrophy of soleus muscle in mice

P.  Minderis; A. Kilikevicius; J. Baltusnikas; Y. Alhindi; T. Venckunas; L. Bunger; A. Lionikas; A. Ratkevicius

doi:10.1111/sms.12532

Myostatin dysfunction is associated with reduction in overload induced hypertrophy of soleus muscle in mice

P. Minderis, A. Kilikevicius, J. Baltusnikas, Y. Alhindi, T. Venckunas, L. Bunger, A. Lionikas, A. Ratkevicius

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Abstract

The aim of the study was to investigate if myostatin dysfunction would promote the gain in muscle mass and peak isometric force (P0) of soleus muscle (SOL) in response to functional overloading (FO) after ablation of the gastrocnemius muscle. Fifteen male Berlin high (BEH) mice homozygous for the compact mutation causing dysfunction of myostatin and 17 mice with the corresponding wild-type allele (BEH+/+) were subjected to FO of SOL for 28 days at the age of 14 weeks. Compared with BEH+/+ mice, SOL of BEH was heavier (mean ± SD, 13.5 ± 1.5 vs 21.4 ± 1.8 mg, respectively, P < 0.001). After FO, SOL mass increased relatively more in BEH+/+ than BEH strain (34.9 ± 11.5 vs 17.7 ± 11.9%, respectively, P < 0.01). P0 fell (P < 0.01) only in BEH strain, which also showed an increase (P < 0.01) in optimal muscle length. Specific P0 became even more depressed in BEH compared with BEH+/+ strain (8.4 ± 1.4 vs 10.8 ± 1.3 N/g, respectively, P < 0.001). Phosphorylation p70 S6 kinase did not differ between the strains. In summary, myostatin dysfunction impairs adaptation of SOL muscle to high functional demands.

Original language	English
Pages (from-to)	894-901
Number of pages	8
Journal	Scandinavian Journal of Medicine & Science in Sports
Volume	26
Issue number	8
Early online date	24 Aug 2015
DOIs	https://doi.org/10.1111/sms.12532
Publication status	Published - Aug 2016

Bibliographical note

Acknowledgements
This project was also supported by Marie Curie International Reintegration Grant 249156 (A. Lionikas) and the grants VP1-3.1-SMM-01-V-02-003 (A. Kilikevicius) and MIP-067/2012 (T. Venckunas) from the Research Council of Lithuania as well as the grant from the Ministry of Higher Education of Saudi Arabia (Y. Alhind). We wish also to thank Mrs Indre Libnickiene for her excellent technical assistance provided during the project.

Keywords

skeletal muscle
muscle hypertrophy
contractile properties
p70S6K
high resistance exercise

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Accepted Manuscript
This is the peer reviewed version of the following article: Minderis, P., Kilikevicius, A., Baltusnikas, J., Alhindi, Y., Venckunas, T., Bunger, L., Lionikas, A. and Ratkevicius, A. (2016), Myostatin dysfunction is associated with reduction in overload induced hypertrophy of soleus muscle in mice. Scandinavian Journal of Medicine & Science in Sports, 26: 894–901., which has been published in final form at doi: 10.1111/sms.12532. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Accepted author manuscript, 1.03 MBLicence: Unspecified

Cite this

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title = "Myostatin dysfunction is associated with reduction in overload induced hypertrophy of soleus muscle in mice",

abstract = "The aim of the study was to investigate if myostatin dysfunction would promote the gain in muscle mass and peak isometric force (P0) of soleus muscle (SOL) in response to functional overloading (FO) after ablation of the gastrocnemius muscle. Fifteen male Berlin high (BEH) mice homozygous for the compact mutation causing dysfunction of myostatin and 17 mice with the corresponding wild-type allele (BEH+/+) were subjected to FO of SOL for 28 days at the age of 14 weeks. Compared with BEH+/+ mice, SOL of BEH was heavier (mean ± SD, 13.5 ± 1.5 vs 21.4 ± 1.8 mg, respectively, P < 0.001). After FO, SOL mass increased relatively more in BEH+/+ than BEH strain (34.9 ± 11.5 vs 17.7 ± 11.9%, respectively, P < 0.01). P0 fell (P < 0.01) only in BEH strain, which also showed an increase (P < 0.01) in optimal muscle length. Specific P0 became even more depressed in BEH compared with BEH+/+ strain (8.4 ± 1.4 vs 10.8 ± 1.3 N/g, respectively, P < 0.001). Phosphorylation p70 S6 kinase did not differ between the strains. In summary, myostatin dysfunction impairs adaptation of SOL muscle to high functional demands.",

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author = "P. Minderis and A. Kilikevicius and J. Baltusnikas and Y. Alhindi and T. Venckunas and L. Bunger and A. Lionikas and A. Ratkevicius",

note = "Acknowledgements This project was also supported by Marie Curie International Reintegration Grant 249156 (A. Lionikas) and the grants VP1-3.1-SMM-01-V-02-003 (A. Kilikevicius) and MIP-067/2012 (T. Venckunas) from the Research Council of Lithuania as well as the grant from the Ministry of Higher Education of Saudi Arabia (Y. Alhind). We wish also to thank Mrs Indre Libnickiene for her excellent technical assistance provided during the project.",

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AU - Minderis, P.

AU - Kilikevicius, A.

AU - Baltusnikas, J.

AU - Alhindi, Y.

AU - Venckunas, T.

AU - Bunger, L.

AU - Lionikas, A.

AU - Ratkevicius, A.

N1 - Acknowledgements This project was also supported by Marie Curie International Reintegration Grant 249156 (A. Lionikas) and the grants VP1-3.1-SMM-01-V-02-003 (A. Kilikevicius) and MIP-067/2012 (T. Venckunas) from the Research Council of Lithuania as well as the grant from the Ministry of Higher Education of Saudi Arabia (Y. Alhind). We wish also to thank Mrs Indre Libnickiene for her excellent technical assistance provided during the project.

PY - 2016/8

Y1 - 2016/8

N2 - The aim of the study was to investigate if myostatin dysfunction would promote the gain in muscle mass and peak isometric force (P0) of soleus muscle (SOL) in response to functional overloading (FO) after ablation of the gastrocnemius muscle. Fifteen male Berlin high (BEH) mice homozygous for the compact mutation causing dysfunction of myostatin and 17 mice with the corresponding wild-type allele (BEH+/+) were subjected to FO of SOL for 28 days at the age of 14 weeks. Compared with BEH+/+ mice, SOL of BEH was heavier (mean ± SD, 13.5 ± 1.5 vs 21.4 ± 1.8 mg, respectively, P < 0.001). After FO, SOL mass increased relatively more in BEH+/+ than BEH strain (34.9 ± 11.5 vs 17.7 ± 11.9%, respectively, P < 0.01). P0 fell (P < 0.01) only in BEH strain, which also showed an increase (P < 0.01) in optimal muscle length. Specific P0 became even more depressed in BEH compared with BEH+/+ strain (8.4 ± 1.4 vs 10.8 ± 1.3 N/g, respectively, P < 0.001). Phosphorylation p70 S6 kinase did not differ between the strains. In summary, myostatin dysfunction impairs adaptation of SOL muscle to high functional demands.

AB - The aim of the study was to investigate if myostatin dysfunction would promote the gain in muscle mass and peak isometric force (P0) of soleus muscle (SOL) in response to functional overloading (FO) after ablation of the gastrocnemius muscle. Fifteen male Berlin high (BEH) mice homozygous for the compact mutation causing dysfunction of myostatin and 17 mice with the corresponding wild-type allele (BEH+/+) were subjected to FO of SOL for 28 days at the age of 14 weeks. Compared with BEH+/+ mice, SOL of BEH was heavier (mean ± SD, 13.5 ± 1.5 vs 21.4 ± 1.8 mg, respectively, P < 0.001). After FO, SOL mass increased relatively more in BEH+/+ than BEH strain (34.9 ± 11.5 vs 17.7 ± 11.9%, respectively, P < 0.01). P0 fell (P < 0.01) only in BEH strain, which also showed an increase (P < 0.01) in optimal muscle length. Specific P0 became even more depressed in BEH compared with BEH+/+ strain (8.4 ± 1.4 vs 10.8 ± 1.3 N/g, respectively, P < 0.001). Phosphorylation p70 S6 kinase did not differ between the strains. In summary, myostatin dysfunction impairs adaptation of SOL muscle to high functional demands.

KW - skeletal muscle

KW - muscle hypertrophy

KW - contractile properties

KW - p70S6K

KW - high resistance exercise

U2 - 10.1111/sms.12532

DO - 10.1111/sms.12532

M3 - Article

SN - 0905-7188

VL - 26

SP - 894

EP - 901

JO - Scandinavian Journal of Medicine & Science in Sports

JF - Scandinavian Journal of Medicine & Science in Sports

IS - 8

ER -

Myostatin dysfunction is associated with reduction in overload induced hypertrophy of soleus muscle in mice

Abstract

Bibliographical note

Keywords

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