NFAT5 genes are part of the osmotic regulatory system in Atlantic salmon (Salmo salar)

Marlene Lorgen; Even H Jørgensen; William C Jordan; Samuel A M Martin; David G Hazlerigg

doi:10.1016/j.margen.2016.06.004

NFAT5 genes are part of the osmotic regulatory system in Atlantic salmon (Salmo salar)

Marlene Lorgen, Even H Jørgensen, William C Jordan, Samuel A M Martin, David G Hazlerigg

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Abstract

The anadromous Atlantic salmon utilizes both fresh and salt water (FW and SW) habitats during its life cycle. The parrsmolt transformation (PST) is an important developmental transition from a FW adapted juvenile parr to a SW adapted
smolt. Physiological changes in osmoregulatory tissues, particularly the gill, are key in maintaining effective ion regulation during PST. Changes are initiated prior to SW exposure (preparative phase), and are completed when smolts enter the
sea (activational phase) where osmotic stress may directly stimulate changes in gene expression. In this paper we identify 4 nuclear factor of activated T cells (NFAT5, an osmotic stress transcription factor) paralogues in Atlantic salmon,
which showed strong homology in characterized functional domains with those identified in other vertebrates. Two of the identified paralogues (NFAT5b1 and NFAT5b2) showed increased expression following transfer from FW to SW. This
effect was largest in parr that were maintained under short day photoperiod, and showed the highest increases in chloride ion levels in response to SW exposure. The results of this study suggest that NFAT5 is involved in the osmotic stress response of Atlantic salmon.

Original language	English
Pages (from-to)	25–31
Number of pages	7
Journal	Marine Genomics
Volume	31
Early online date	18 Jun 2016
DOIs	https://doi.org/10.1016/j.margen.2016.06.004
Publication status	Published - 1 Feb 2017

Bibliographical note

Acknowledgements
This study was supported by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC, BB/H008063/1), UK to DGH and SAM. Funding also came from Research Council Norway for project number 241016 for DGH and EJ. This work was carried out as part of a PhD thesis funded by the Marine Alliance of Science and Technology Scotland (MASTS).

Keywords

nuclear activated factor of T-cells
salinity
salmonid
thyroid hormone
whole genome duplication

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10.1016/j.margen.2016.06.004Licence: CC BY

NFAT5 genes are part of the osmotic regulatory system in Atlantic salmon
© 2016 The Authors. Published by Elsevier B.V. This publication is Open Access funded by Biotechnology and Biological Sciences Research Council under a Creative Commons licence https://creativecommons.org/licenses/by/4.0/
Final published version, 605 KBLicence: CC BY

Cite this

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title = "NFAT5 genes are part of the osmotic regulatory system in Atlantic salmon (Salmo salar)",

abstract = "The anadromous Atlantic salmon utilizes both fresh and salt water (FW and SW) habitats during its life cycle. The parrsmolt transformation (PST) is an important developmental transition from a FW adapted juvenile parr to a SW adaptedsmolt. Physiological changes in osmoregulatory tissues, particularly the gill, are key in maintaining effective ion regulation during PST. Changes are initiated prior to SW exposure (preparative phase), and are completed when smolts enter thesea (activational phase) where osmotic stress may directly stimulate changes in gene expression. In this paper we identify 4 nuclear factor of activated T cells (NFAT5, an osmotic stress transcription factor) paralogues in Atlantic salmon,which showed strong homology in characterized functional domains with those identified in other vertebrates. Two of the identified paralogues (NFAT5b1 and NFAT5b2) showed increased expression following transfer from FW to SW. Thiseffect was largest in parr that were maintained under short day photoperiod, and showed the highest increases in chloride ion levels in response to SW exposure. The results of this study suggest that NFAT5 is involved in the osmotic stress response of Atlantic salmon.",

keywords = "nuclear activated factor of T-cells, salinity, salmonid, thyroid hormone, whole genome duplication",

author = "Marlene Lorgen and J{\o}rgensen, {Even H} and Jordan, {William C} and Martin, {Samuel A M} and Hazlerigg, {David G}",

note = "Acknowledgements This study was supported by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC, BB/H008063/1), UK to DGH and SAM. Funding also came from Research Council Norway for project number 241016 for DGH and EJ. This work was carried out as part of a PhD thesis funded by the Marine Alliance of Science and Technology Scotland (MASTS).",

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AU - Lorgen, Marlene

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AU - Jordan, William C

AU - Martin, Samuel A M

AU - Hazlerigg, David G

N1 - Acknowledgements This study was supported by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC, BB/H008063/1), UK to DGH and SAM. Funding also came from Research Council Norway for project number 241016 for DGH and EJ. This work was carried out as part of a PhD thesis funded by the Marine Alliance of Science and Technology Scotland (MASTS).

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N2 - The anadromous Atlantic salmon utilizes both fresh and salt water (FW and SW) habitats during its life cycle. The parrsmolt transformation (PST) is an important developmental transition from a FW adapted juvenile parr to a SW adaptedsmolt. Physiological changes in osmoregulatory tissues, particularly the gill, are key in maintaining effective ion regulation during PST. Changes are initiated prior to SW exposure (preparative phase), and are completed when smolts enter thesea (activational phase) where osmotic stress may directly stimulate changes in gene expression. In this paper we identify 4 nuclear factor of activated T cells (NFAT5, an osmotic stress transcription factor) paralogues in Atlantic salmon,which showed strong homology in characterized functional domains with those identified in other vertebrates. Two of the identified paralogues (NFAT5b1 and NFAT5b2) showed increased expression following transfer from FW to SW. Thiseffect was largest in parr that were maintained under short day photoperiod, and showed the highest increases in chloride ion levels in response to SW exposure. The results of this study suggest that NFAT5 is involved in the osmotic stress response of Atlantic salmon.

AB - The anadromous Atlantic salmon utilizes both fresh and salt water (FW and SW) habitats during its life cycle. The parrsmolt transformation (PST) is an important developmental transition from a FW adapted juvenile parr to a SW adaptedsmolt. Physiological changes in osmoregulatory tissues, particularly the gill, are key in maintaining effective ion regulation during PST. Changes are initiated prior to SW exposure (preparative phase), and are completed when smolts enter thesea (activational phase) where osmotic stress may directly stimulate changes in gene expression. In this paper we identify 4 nuclear factor of activated T cells (NFAT5, an osmotic stress transcription factor) paralogues in Atlantic salmon,which showed strong homology in characterized functional domains with those identified in other vertebrates. Two of the identified paralogues (NFAT5b1 and NFAT5b2) showed increased expression following transfer from FW to SW. Thiseffect was largest in parr that were maintained under short day photoperiod, and showed the highest increases in chloride ion levels in response to SW exposure. The results of this study suggest that NFAT5 is involved in the osmotic stress response of Atlantic salmon.

KW - nuclear activated factor of T-cells

KW - salinity

KW - salmonid

KW - thyroid hormone

KW - whole genome duplication

U2 - 10.1016/j.margen.2016.06.004

DO - 10.1016/j.margen.2016.06.004

M3 - Article

SN - 1874-7787

VL - 31

SP - 25

EP - 31

JO - Marine Genomics

JF - Marine Genomics

ER -

NFAT5 genes are part of the osmotic regulatory system in Atlantic salmon (Salmo salar)

Abstract

Bibliographical note

Keywords

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