Nitric oxide synthesis in patients with infective gastroenteritis

P  Forte; R S  Dykhuizen; Eric Milne; A  McKenzie; C C  Smith; N  Benjamin

doi:10.1136/gut.45.3.355

Nitric oxide synthesis in patients with infective gastroenteritis

P Forte, R S Dykhuizen, Eric Milne, A McKenzie, C C Smith, N Benjamin

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Background-There is evidence that endogenous nitrate synthesis is notably increased in patients with infective gastroenteritis.

Aims-To determine whether this is due to nitric oxide (NO) production via the L-arginine/NO pathway.

Methods-Seven male patients with community acquired bacterial gastroenteritis and 15 healthy male volunteers participated in this study, All patients had stool culture positive infective gastroenteritis. A bolus of 200 mg L-[N-15](2)-arginine was administered intravenously after an overnight fast. Urine was collected for the next 36 hours. Urinary [N-15:N-14]nitrate ratio was assessed by dry combustion in an isotope ratio mass spectrometer.

Results-Mean 36 hour total urinary nitrate excretion in the gastroenteritis group was 5157 (577) mu mol compared with 2594 (234) mu mol in the control group (p<0.001). Thirty six hour urinary [N-15]nitrate excretion was considerably higher in the gastroenteritis group compared with the control group (13782 (1665) versus 1698 (98) eta mol; p<0.001). These values represent 1.129 (0.139)% and 0.138 (0.007)% of [N-15]nitrogen administered (p<0.001), respectively. Corrected 36 hour urinary [N-15]nitrate excretion for urinary creatinine was also significantly higher in the patient compared with the control group (1934 (221) versus 303 (35) eta mol/mmol; p<0.001).

Conclusion-Results show notably enhanced nitrate synthesis due to increased activity of the L-arginine/NO pathway in patients with infective gastroenteritis.

Original language	English
Pages (from-to)	355-361
Number of pages	7
Journal	Gut
Volume	45
Issue number	3
DOIs	https://doi.org/10.1136/gut.45.3.355
Publication status	Published - Sept 1999

Keywords

endothelium derived relaxing factor
L-[N-15](2)-arginine
nitrates
infection
diarrhoea
inflammatory bowel-disease
colon epithelial-cells
plasma nitrate concentration
active ulcerative-colitis
challenge in-vivo
synthase activity
L-arginine
bacterial translocation
Chrons-Disease
endotoxin challenge

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1136/gut.45.3.355

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@article{98d9d262f5284970a5fa6c660421828a,

title = "Nitric oxide synthesis in patients with infective gastroenteritis",

abstract = "Background-There is evidence that endogenous nitrate synthesis is notably increased in patients with infective gastroenteritis.Aims-To determine whether this is due to nitric oxide (NO) production via the L-arginine/NO pathway.Methods-Seven male patients with community acquired bacterial gastroenteritis and 15 healthy male volunteers participated in this study, All patients had stool culture positive infective gastroenteritis. A bolus of 200 mg L-[N-15](2)-arginine was administered intravenously after an overnight fast. Urine was collected for the next 36 hours. Urinary [N-15:N-14]nitrate ratio was assessed by dry combustion in an isotope ratio mass spectrometer.Results-Mean 36 hour total urinary nitrate excretion in the gastroenteritis group was 5157 (577) mu mol compared with 2594 (234) mu mol in the control group (p<0.001). Thirty six hour urinary [N-15]nitrate excretion was considerably higher in the gastroenteritis group compared with the control group (13782 (1665) versus 1698 (98) eta mol; p<0.001). These values represent 1.129 (0.139)% and 0.138 (0.007)% of [N-15]nitrogen administered (p<0.001), respectively. Corrected 36 hour urinary [N-15]nitrate excretion for urinary creatinine was also significantly higher in the patient compared with the control group (1934 (221) versus 303 (35) eta mol/mmol; p<0.001).Conclusion-Results show notably enhanced nitrate synthesis due to increased activity of the L-arginine/NO pathway in patients with infective gastroenteritis.",

keywords = "endothelium derived relaxing factor, L-[N-15](2)-arginine, nitrates, infection, diarrhoea, inflammatory bowel-disease, colon epithelial-cells, plasma nitrate concentration, active ulcerative-colitis, challenge in-vivo, synthase activity, L-arginine, bacterial translocation, Chrons-Disease, endotoxin challenge",

author = "P Forte and Dykhuizen, {R S} and Eric Milne and A McKenzie and Smith, {C C} and N Benjamin",

year = "1999",

month = sep,

doi = "10.1136/gut.45.3.355",

language = "English",

volume = "45",

pages = "355--361",

journal = "Gut",

issn = "0017-5749",

publisher = "BMJ Publishing Group",

number = "3",

}

TY - JOUR

T1 - Nitric oxide synthesis in patients with infective gastroenteritis

AU - Forte, P

AU - Dykhuizen, R S

AU - Milne, Eric

AU - McKenzie, A

AU - Smith, C C

AU - Benjamin, N

PY - 1999/9

Y1 - 1999/9

N2 - Background-There is evidence that endogenous nitrate synthesis is notably increased in patients with infective gastroenteritis.Aims-To determine whether this is due to nitric oxide (NO) production via the L-arginine/NO pathway.Methods-Seven male patients with community acquired bacterial gastroenteritis and 15 healthy male volunteers participated in this study, All patients had stool culture positive infective gastroenteritis. A bolus of 200 mg L-[N-15](2)-arginine was administered intravenously after an overnight fast. Urine was collected for the next 36 hours. Urinary [N-15:N-14]nitrate ratio was assessed by dry combustion in an isotope ratio mass spectrometer.Results-Mean 36 hour total urinary nitrate excretion in the gastroenteritis group was 5157 (577) mu mol compared with 2594 (234) mu mol in the control group (p<0.001). Thirty six hour urinary [N-15]nitrate excretion was considerably higher in the gastroenteritis group compared with the control group (13782 (1665) versus 1698 (98) eta mol; p<0.001). These values represent 1.129 (0.139)% and 0.138 (0.007)% of [N-15]nitrogen administered (p<0.001), respectively. Corrected 36 hour urinary [N-15]nitrate excretion for urinary creatinine was also significantly higher in the patient compared with the control group (1934 (221) versus 303 (35) eta mol/mmol; p<0.001).Conclusion-Results show notably enhanced nitrate synthesis due to increased activity of the L-arginine/NO pathway in patients with infective gastroenteritis.

AB - Background-There is evidence that endogenous nitrate synthesis is notably increased in patients with infective gastroenteritis.Aims-To determine whether this is due to nitric oxide (NO) production via the L-arginine/NO pathway.Methods-Seven male patients with community acquired bacterial gastroenteritis and 15 healthy male volunteers participated in this study, All patients had stool culture positive infective gastroenteritis. A bolus of 200 mg L-[N-15](2)-arginine was administered intravenously after an overnight fast. Urine was collected for the next 36 hours. Urinary [N-15:N-14]nitrate ratio was assessed by dry combustion in an isotope ratio mass spectrometer.Results-Mean 36 hour total urinary nitrate excretion in the gastroenteritis group was 5157 (577) mu mol compared with 2594 (234) mu mol in the control group (p<0.001). Thirty six hour urinary [N-15]nitrate excretion was considerably higher in the gastroenteritis group compared with the control group (13782 (1665) versus 1698 (98) eta mol; p<0.001). These values represent 1.129 (0.139)% and 0.138 (0.007)% of [N-15]nitrogen administered (p<0.001), respectively. Corrected 36 hour urinary [N-15]nitrate excretion for urinary creatinine was also significantly higher in the patient compared with the control group (1934 (221) versus 303 (35) eta mol/mmol; p<0.001).Conclusion-Results show notably enhanced nitrate synthesis due to increased activity of the L-arginine/NO pathway in patients with infective gastroenteritis.

KW - endothelium derived relaxing factor

KW - L-[N-15](2)-arginine

KW - nitrates

KW - infection

KW - diarrhoea

KW - inflammatory bowel-disease

KW - colon epithelial-cells

KW - plasma nitrate concentration

KW - active ulcerative-colitis

KW - challenge in-vivo

KW - synthase activity

KW - L-arginine

KW - bacterial translocation

KW - Chrons-Disease

KW - endotoxin challenge

U2 - 10.1136/gut.45.3.355

DO - 10.1136/gut.45.3.355

M3 - Article

SN - 0017-5749

VL - 45

SP - 355

EP - 361

JO - Gut

JF - Gut

IS - 3

ER -

Nitric oxide synthesis in patients with infective gastroenteritis

Abstract

Keywords

UN SDGs

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