Abstract
Two obligately acidophilic, mesophilic and aerobic soil ammonia-oxidising archaea were isolated from a pH 4.5 arable sandy loam (UK) and pH 4.7 acidic sulphate paddy soil (China)
and designated strains Nd1T and Nd2T, respectively. Strains share >99% 16S rRNA gene sequence identity and their genomes are <2 Mb in length, sharing 79% average nucleotide identity, 81% average amino acid identity and a G+C mol% ~37%. Strains are chemolithotrophs that fix carbon dioxide and gain energy by oxidising ammonia to nitrite with no evidence of mixotrophic growth. Neither strain is capable of using urea as a source of
ammonia. Strains are non-motile in culture although Nd1T does possess genes encoding flagella and therefore may be motile under certain conditions. Cells are small angular rods 0.5 - 1 μm in length and grow in the pH range 4.0 – 6.1 and temperature range 20 – 40o C. Strains Nd1T and Nd2T are distinct in genomic and physiological features and are assigned as the type strains for the species Nitrosotalea devaniterrae sp. nov. (type strain, Nd1T=NCIMB 15248T=DSM110862T) and Nitrosotalea sinensis sp. nov. (type strain, Nd2T=NCIMB 15249T=DSM110863T), respectively, within the genus Nitrosotalea gen. nov. The family Nitrosotaleaceae fam. nov. and order Nitrosotaleales ord. nov. are also proposed officially.
and designated strains Nd1T and Nd2T, respectively. Strains share >99% 16S rRNA gene sequence identity and their genomes are <2 Mb in length, sharing 79% average nucleotide identity, 81% average amino acid identity and a G+C mol% ~37%. Strains are chemolithotrophs that fix carbon dioxide and gain energy by oxidising ammonia to nitrite with no evidence of mixotrophic growth. Neither strain is capable of using urea as a source of
ammonia. Strains are non-motile in culture although Nd1T does possess genes encoding flagella and therefore may be motile under certain conditions. Cells are small angular rods 0.5 - 1 μm in length and grow in the pH range 4.0 – 6.1 and temperature range 20 – 40o C. Strains Nd1T and Nd2T are distinct in genomic and physiological features and are assigned as the type strains for the species Nitrosotalea devaniterrae sp. nov. (type strain, Nd1T=NCIMB 15248T=DSM110862T) and Nitrosotalea sinensis sp. nov. (type strain, Nd2T=NCIMB 15249T=DSM110863T), respectively, within the genus Nitrosotalea gen. nov. The family Nitrosotaleaceae fam. nov. and order Nitrosotaleales ord. nov. are also proposed officially.
| Original language | English |
|---|---|
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Publication status | Accepted/In press - 8 May 2024 |