Pax9 and Gbx2 Interact in the Pharyngeal Endoderm to Control Cardiovascular Development

Catherine A. Stothard, Silvia Mazzotta, Arjun Vyas, Jurgen E. Schneider, Timothy J. Mohun, Deborah J. Henderson, Helen M. Phillips, Simon D. Bamforth

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Abstract

The correct formation of the aortic arch arteries depends on a coordinated and regulated gene expression profile within the tissues of the pharyngeal arches. Perturbation of the gene regulatory networks in these tissues results in congenital heart defects affecting the arch arteries and the outflow tract of the heart. Aberrant development of these structures leads to interruption of the aortic arch and double outlet right ventricle, abnormalities that are a leading cause of morbidity in 22q11 Deletion Syndrome (DS) patients. We have recently shown that Pax9 functionally interacts with the 22q11DS gene Tbx1 in the pharyngeal endoderm for 4th pharyngeal arch artery morphogenesis, with double heterozygous mice dying at birth with interrupted aortic arch. Mice lacking Pax9 die perinatally with complex cardiovascular defects and in this study we sought to validate further potential genetic interacting partners of Pax9, focussing on Gbx2 which is down-regulated in the pharyngeal endoderm of Pax9-null embryos. Here, we describe the Gbx2-null cardiovascular phenotype and demonstrate a genetic interaction between Gbx2 and Pax9 in the pharyngeal endoderm during cardiovascular development.
Original languageEnglish
Article number20
JournalJournal of Cardiovascular Development and Disease
Volume7
Issue number2
Early online date25 May 2020
DOIs
Publication statusPublished - 1 Jun 2020

Bibliographical note

Funding: T
his research was funded by a British Heart Foundation project grant (PG/16/39/32115; to SDB) and a
British Heart Foundation Non-Clinical PhD Studentship (FS/16/8/31984; to SDB). JES would like to acknowledge
infrastructure funding from the British Heart Foundation, UK (SI/14/1/30718).
Acknowledgments:
The 2xTk-GL2 and BMP4-luc control plasmids were gifts from Anita Rauch and Rena D’Souza.
The Gbx2 and Pitx2 probes were gifts from Alexandra Joyner and Dominic Norris. We acknowledge the Newcastle
University Flow Cytometry Core Facility (FCCF) for assistance with the generation of the flow cytometry data.

Keywords

  • Arch arteries
  • Gbx2
  • Pax9
  • Pharyngeal endoderm
  • Tbx1

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