Pharmacological GSK-3beta inhibition improves osteoblast differentiation on titanium surfaces

Simone Lumetti, Sara Ferrillo, Silvia Mazzotta, Guido Maria Macaluso, Mauro Bonanini, Giovanni Passeri, Carlo Galli

Research output: Contribution to journalArticlepeer-review


Rough titanium surfaces enhance the activation of Wnt canonical signaling, a pathway required for osteoblast differentiation. The present study investigated the effects of GSK3b-inhibitor (2'Z,3'E)- 6-Bromoindirubin-3'-oxime (BIO) on osteoblastic differentiation on titanium surfaces with different topography and wettability. C2C12 cells were plated on pickled, acid-etched/sand-blasted (SLA), modified hydrophilic SLA titanium discs (modSLA) and stimulated with increasing doses of BIO. Activation of Wnt canonical signaling was measured with a reporter system. Gene expression was measured in the same cell system by Real Time PCR. Osteoblastic MC3T3 cells were then plated on discs with or without BIO and the expression of osteoblast specific genes was assessed by Real Time PCR. One mM BIO activated Wnt canonical signaling in C2C12 cells on all surfaces, and the highest effect was on rough surfaces. BIO markedly increased the expression of Osteoprotegerin and Osteocalcin in MC3T3 cells on rough surfaces at the concentration of 100 nM, and on all surfaces at the concentration of 1 mM. BIO enhances Wnt signaling activation and the expression of osteoblastic genes on rough surfaces and could be a viable approach to improve cell response to implant surfaces.
Original languageEnglish
Pages (from-to)489-495
JournalJournal of Biological Regulators and Homeostatic Agents
Issue number3
Publication statusPublished - 2014


Dive into the research topics of 'Pharmacological GSK-3beta inhibition improves osteoblast differentiation on titanium surfaces'. Together they form a unique fingerprint.

Cite this