Physicochemical Characterization of Acidiphilium sp. Biofilms

Moustafa Malki, Santiago Casado, Maria Francisca Lopez, Renaud Caillard, F. Javier Palomares, Jose A. Martin Gago, Cristina Vaz-Dominguez, Angel Cuesta, Ricardo Amils, Victor M. Fernandez, Marisela Velez, Antonio L. De Lacey, David Olea

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


The biofilm formation of a strain of the extremophile bacterium Acidiphilium sp., capable of donating electrons directly to electrodes, was studied by different surface characterization techniques. We develop a method that allows the simultaneous study of bacterial biofilms by means of fluorescence microscopy and atomic force microscopy (AFM), in which transparent graphitic flakes deposited on a glass substrate are used as a support for the biofilm. The majority of the cells present on the surface were viable, and the growth of the biofilms over time showed a critical increase of the extracellular polymeric substances (EPS) as well as the formation of nanosized particles inside the biofilm. Also, the presence of Fe in Acidiphilium biofilms was determined by X-ray photoelectron spectroscopy (XPS), whereas surface-enhanced infrared absorption spectroscopy indicated the presence of redox-active proteins.
Original languageEnglish
Pages (from-to)1237-1244
Number of pages8
Issue number6
Early online date1 Mar 2013
Publication statusPublished - 15 Apr 2013


  • atomic force microscopy
  • biofilms
  • graphite
  • redox reactions
  • X-ray photoelectron spectroscopy


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