From 2013–2017, 11 chestnut populations and 16 apple orchards/plantations in Macedonia were examined for health; soil, root and bark samples were collected from trees expressing symptoms regarded as Phytophthora specific. Using leaf baits of Prunus laurocerasus and selective V8 Agar (PARPNH), 19 pure Phytophthora sp. cultures were isolated and identified as P. cactorum by ITS sequencing. Sixteen isolates were from apple trees and 3 from chestnut trees. Phylogenetic analyses suggested slight distance between P. cactorum isolates originating from chestnut trees compared to those from apple orchards. Assessment of pathogenicity using chestnuts twigs showed no differences between P. cactorum isolates from the two tree host species.
|Number of pages||11|
|Journal||Macedonian Academy of Sciences and Arts, Section: Natural, Mathematical and Biotechnical Sciences|
|Publication status||Published - 2019|
Bibliographical noteAcknowledgments. SCOPES Joint Research Project "Invasive chestnut diseases in the Balkans and Georgia - epidemiological research and management options".
Multilateral project (Switzerland, Croatia, Macedonia and Georgia), 2013-2016 (IZ73Z0_152525).
SEE-ERA.NET PLUS Joint Research Project "Diversity and invading Phytophthora spp. plant pathogens in agro and forest ecosystems in Southeast Europe". Multilateral project (Bulgaria, Macedonia, Greece, Serbia and Romania), 2010-2012.
COST (Cost Action FP0801: Established and Emerging Phytophthora: Increasing Threats to Woodland and Forest Ecosystems.)
- Malus spp.
- Castanea sativa
- phylogenetic analysis