Abstract
BACKGROUND
Miscanthus×giganteus (M.×giganteus) is a potential source for bioethanol or other useful products. Pretreatment of lignocellulosic biomass is an essential step prior to enzymatic hydrolysis to sugars and fermentation to bioethanol.
RESULTS
In this work, a one-step process uses aqueous ammonia with or without hydrogen peroxide; a proposed two-step process uses aqueous ammonia in the first step and hydrogen peroxide in the second step. In the two-step process, overall 89.5% lignin is removed. The pretreated biomass is followed by using cellulase and β-glucosidase to convert cellulose and hemicellulose from the recovered solid to fermentable sugars. The conversion of cellulose to glucose is 90.2% and to xylose is 73.4%. Characterization data are obtained for the recovered solid using scanning electron microscopy (SEM), attenuated total reflection-infrared spectroscopy (ATR-IR), and X-ray diffraction (XRD) for better understanding of the two-step process.
CONCLUSION
Results from the two-step process using aqueous ammonia and hydrogen peroxide separately are much better than those from the one-step process for removing lignin and for enhancing conversion to sugars by enzymatic hydrolysis. © 2013 Society of Chemical Industry
Miscanthus×giganteus (M.×giganteus) is a potential source for bioethanol or other useful products. Pretreatment of lignocellulosic biomass is an essential step prior to enzymatic hydrolysis to sugars and fermentation to bioethanol.
RESULTS
In this work, a one-step process uses aqueous ammonia with or without hydrogen peroxide; a proposed two-step process uses aqueous ammonia in the first step and hydrogen peroxide in the second step. In the two-step process, overall 89.5% lignin is removed. The pretreated biomass is followed by using cellulase and β-glucosidase to convert cellulose and hemicellulose from the recovered solid to fermentable sugars. The conversion of cellulose to glucose is 90.2% and to xylose is 73.4%. Characterization data are obtained for the recovered solid using scanning electron microscopy (SEM), attenuated total reflection-infrared spectroscopy (ATR-IR), and X-ray diffraction (XRD) for better understanding of the two-step process.
CONCLUSION
Results from the two-step process using aqueous ammonia and hydrogen peroxide separately are much better than those from the one-step process for removing lignin and for enhancing conversion to sugars by enzymatic hydrolysis. © 2013 Society of Chemical Industry
Original language | English |
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Pages (from-to) | 698-706 |
Number of pages | 9 |
Journal | Journal of Chemical Technology & Biotechnology |
Volume | 89 |
Issue number | 5 |
DOIs | |
Publication status | Published - May 2014 |
Keywords
- Miscanthus×giganteus
- pretreatment
- aqueous ammonia
- hydrogen peroxide
- enzymatic hydrolysis