Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats.

M. Brookes; N. Aegerter; C. Smith; M. Healy; A. Jolliffe; Susan Swift; I. J. Mackie; J. S. Pritchard; Paul Adrian Racey; P. Moore; A. R. Fooks

Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats.

M. Brookes, N. Aegerter, C. Smith, M. Healy, A. Jolliffe, Susan Swift, I. J. Mackie, J. S. Pritchard, Paul Adrian Racey, P. Moore, A. R. Fooks

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

We report the first seroprevalence study of the occurrence of specific antibodies to European bat lyssavirus type 2 (EBLV-2) in Daubenton's bats. Bats were captured from 19 sites across eastern and southern Scotland. Samples from 198 Daubenton's bats, 20 Natterer's bats, and 6 Pipistrelle's bats were tested for EBLV-2. Blood samples (N = 94) were subjected to a modified fluorescent antibody virus neutralization test to determine antibody titer. From 0.05% to 3.8% (95% confidence interval) of Daubenton's bats were seropositive. Antibodies to EBLV-2 were not detected in the 2 other species tested. Mouth swabs (N = 218) were obtained, and RNA was extracted for a reverse transcription-polymerase chain reaction (RTPCR). The RT-PCR included pan lyssavirus-primers (N gene) and internal PCR control primers for ribosomal RNA. EBLV-2 RNA was not detected in any of the saliva samples tested, and live virus was not detected in virus isolation tests.

Original language	English
Pages (from-to)	572-578
Number of pages	6
Journal	Emerging Infectious Diseases
Volume	11
Publication status	Published - 2005

Keywords

RABIES-RELATED VIRUSES
DAUBENTONS BAT
UNITED-KINGDOM
TYPE-2
IDENTIFICATION
POPULATIONS
INFECTION
PCR
UK

Cite this

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title = "Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats.",

abstract = "We report the first seroprevalence study of the occurrence of specific antibodies to European bat lyssavirus type 2 (EBLV-2) in Daubenton's bats. Bats were captured from 19 sites across eastern and southern Scotland. Samples from 198 Daubenton's bats, 20 Natterer's bats, and 6 Pipistrelle's bats were tested for EBLV-2. Blood samples (N = 94) were subjected to a modified fluorescent antibody virus neutralization test to determine antibody titer. From 0.05% to 3.8% (95% confidence interval) of Daubenton's bats were seropositive. Antibodies to EBLV-2 were not detected in the 2 other species tested. Mouth swabs (N = 218) were obtained, and RNA was extracted for a reverse transcription-polymerase chain reaction (RTPCR). The RT-PCR included pan lyssavirus-primers (N gene) and internal PCR control primers for ribosomal RNA. EBLV-2 RNA was not detected in any of the saliva samples tested, and live virus was not detected in virus isolation tests.",

keywords = "RABIES-RELATED VIRUSES, DAUBENTONS BAT, UNITED-KINGDOM, TYPE-2, IDENTIFICATION, POPULATIONS, INFECTION, PCR, UK",

author = "M. Brookes and N. Aegerter and C. Smith and M. Healy and A. Jolliffe and Susan Swift and Mackie, {I. J.} and Pritchard, {J. S.} and Racey, {Paul Adrian} and P. Moore and Fooks, {A. R.}",

year = "2005",

language = "English",

volume = "11",

pages = "572--578",

journal = "Emerging Infectious Diseases",

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T1 - Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats.

AU - Brookes, M.

AU - Aegerter, N.

AU - Smith, C.

AU - Healy, M.

AU - Jolliffe, A.

AU - Swift, Susan

AU - Mackie, I. J.

AU - Pritchard, J. S.

AU - Racey, Paul Adrian

AU - Moore, P.

AU - Fooks, A. R.

PY - 2005

Y1 - 2005

N2 - We report the first seroprevalence study of the occurrence of specific antibodies to European bat lyssavirus type 2 (EBLV-2) in Daubenton's bats. Bats were captured from 19 sites across eastern and southern Scotland. Samples from 198 Daubenton's bats, 20 Natterer's bats, and 6 Pipistrelle's bats were tested for EBLV-2. Blood samples (N = 94) were subjected to a modified fluorescent antibody virus neutralization test to determine antibody titer. From 0.05% to 3.8% (95% confidence interval) of Daubenton's bats were seropositive. Antibodies to EBLV-2 were not detected in the 2 other species tested. Mouth swabs (N = 218) were obtained, and RNA was extracted for a reverse transcription-polymerase chain reaction (RTPCR). The RT-PCR included pan lyssavirus-primers (N gene) and internal PCR control primers for ribosomal RNA. EBLV-2 RNA was not detected in any of the saliva samples tested, and live virus was not detected in virus isolation tests.

AB - We report the first seroprevalence study of the occurrence of specific antibodies to European bat lyssavirus type 2 (EBLV-2) in Daubenton's bats. Bats were captured from 19 sites across eastern and southern Scotland. Samples from 198 Daubenton's bats, 20 Natterer's bats, and 6 Pipistrelle's bats were tested for EBLV-2. Blood samples (N = 94) were subjected to a modified fluorescent antibody virus neutralization test to determine antibody titer. From 0.05% to 3.8% (95% confidence interval) of Daubenton's bats were seropositive. Antibodies to EBLV-2 were not detected in the 2 other species tested. Mouth swabs (N = 218) were obtained, and RNA was extracted for a reverse transcription-polymerase chain reaction (RTPCR). The RT-PCR included pan lyssavirus-primers (N gene) and internal PCR control primers for ribosomal RNA. EBLV-2 RNA was not detected in any of the saliva samples tested, and live virus was not detected in virus isolation tests.

KW - RABIES-RELATED VIRUSES

KW - DAUBENTONS BAT

KW - UNITED-KINGDOM

KW - TYPE-2

KW - IDENTIFICATION

KW - POPULATIONS

KW - INFECTION

KW - PCR

KW - UK

M3 - Article

SN - 1080-6040

VL - 11

SP - 572

EP - 578

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

ER -

Prevalence of Antibodies to European Bat Lyssavirus Type 2 in Scottish Bats.

Abstract

Keywords

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