Abstract
Techniques used to generate mutants in Candida albicans commonly result in additional and undesired genetic rearrangements. Detection of aneuploidy is, therefore, an important step forward in the quality control of mutant phenotypes. In this chapter, we describe how to extract genomic DNA and perform a quantitative multiplex PCR to compare the karyotype of any mutant strain to that of its parent and allow the detection of any unwanted aneuploidy.
Original language | English |
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Pages (from-to) | 41-49 |
Number of pages | 9 |
Journal | Methods in Molecular Biology |
Volume | 845 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2012 |