Rapid method for quantification of anthocyanidins and anthocyanins in human biological samples

Deepak M. Kasote; Gary J. Duncan; Madalina Neacsu; Wendy R. Russell

doi:10.1016/j.foodchem.2019.03.109

Rapid method for quantification of anthocyanidins and anthocyanins in human biological samples

Deepak M. Kasote^* (Corresponding Author), Gary J. Duncan, Madalina Neacsu, Wendy R. Russell^* (Corresponding Author)

^*Corresponding author for this work

The Rowett Institute of Nutrition and Health

University of Aberdeen

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

4 Downloads (Pure)

Abstract

Using simple solvent extraction and enzymatic hydrolysis, a rapid LC-MS/MS method for quantification of free and conjugated forms of anthocyanidins and anthocyanins in plasma and urine samples was developed and validated. A mixed enzymatic treatment containing β-glucuronidase (100 U mL ⁻¹ ) and sulfatase (2.5 U mL ⁻¹ ) for 5 min (37 °C; pH 6) was optimal condition for deconjugation of anthocyanidins and anthocyanins in urine and plasma samples. The LC–MS/MS allowed quantifying thirteen different anthocyanidins and anthocyanins simultaneously. The developed LC–MS/MS method was precise and accurate over multiple days and nominal concentrations. The stability assessment study confirmed that the long-term storage and/or periodic use of plasma and urine samples might have a considerable impact on the stability of some anthocyanidins. The method was successfully applied to measure anthocyanidins and anthocyanins in plasma and urine samples following consumption of acute blueberry test meals.

Original language	English
Pages (from-to)	56-63
Number of pages	8
Journal	Food Chemistry
Volume	290
Early online date	21 Mar 2019
DOIs	https://doi.org/10.1016/j.foodchem.2019.03.109
Publication status	Published - 30 Aug 2019

Bibliographical note

This work was performed with the support of “The Scottish Government's Rural and Environment Science and Analytical Services Division (RESAS),” Scotland, U.K.

Keywords

Acute blueberry consumption
Anthocyanidin
Anthocyanin
Plasma
Urine
MASS-SPECTROMETRY METHOD
LIQUID-CHROMATOGRAPHY
HUMAN PLASMA
URINE
VALIDATION
BIOAVAILABILITY

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Accepted Manuscript
© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Accepted author manuscript, 745 KBLicence: CC BY-NC-ND

Cite this

@article{eb532d3792d94377864f20183a955660,

title = "Rapid method for quantification of anthocyanidins and anthocyanins in human biological samples",

abstract = " Using simple solvent extraction and enzymatic hydrolysis, a rapid LC-MS/MS method for quantification of free and conjugated forms of anthocyanidins and anthocyanins in plasma and urine samples was developed and validated. A mixed enzymatic treatment containing β-glucuronidase (100 U mL −1 ) and sulfatase (2.5 U mL −1 ) for 5 min (37 °C; pH 6) was optimal condition for deconjugation of anthocyanidins and anthocyanins in urine and plasma samples. The LC–MS/MS allowed quantifying thirteen different anthocyanidins and anthocyanins simultaneously. The developed LC–MS/MS method was precise and accurate over multiple days and nominal concentrations. The stability assessment study confirmed that the long-term storage and/or periodic use of plasma and urine samples might have a considerable impact on the stability of some anthocyanidins. The method was successfully applied to measure anthocyanidins and anthocyanins in plasma and urine samples following consumption of acute blueberry test meals. ",

keywords = "Acute blueberry consumption, Anthocyanidin, Anthocyanin, Plasma, Urine, MASS-SPECTROMETRY METHOD, LIQUID-CHROMATOGRAPHY, HUMAN PLASMA, URINE, VALIDATION, BIOAVAILABILITY",

author = "Kasote, {Deepak M.} and Duncan, {Gary J.} and Madalina Neacsu and Russell, {Wendy R.}",

note = "This work was performed with the support of “The Scottish Government's Rural and Environment Science and Analytical Services Division (RESAS),” Scotland, U.K.",

year = "2019",

month = aug,

day = "30",

doi = "10.1016/j.foodchem.2019.03.109",

language = "English",

volume = "290",

pages = "56--63",

journal = "Food Chemistry",

issn = "0308-8146",

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AU - Kasote, Deepak M.

AU - Duncan, Gary J.

AU - Neacsu, Madalina

AU - Russell, Wendy R.

N1 - This work was performed with the support of “The Scottish Government's Rural and Environment Science and Analytical Services Division (RESAS),” Scotland, U.K.

PY - 2019/8/30

Y1 - 2019/8/30

N2 - Using simple solvent extraction and enzymatic hydrolysis, a rapid LC-MS/MS method for quantification of free and conjugated forms of anthocyanidins and anthocyanins in plasma and urine samples was developed and validated. A mixed enzymatic treatment containing β-glucuronidase (100 U mL −1 ) and sulfatase (2.5 U mL −1 ) for 5 min (37 °C; pH 6) was optimal condition for deconjugation of anthocyanidins and anthocyanins in urine and plasma samples. The LC–MS/MS allowed quantifying thirteen different anthocyanidins and anthocyanins simultaneously. The developed LC–MS/MS method was precise and accurate over multiple days and nominal concentrations. The stability assessment study confirmed that the long-term storage and/or periodic use of plasma and urine samples might have a considerable impact on the stability of some anthocyanidins. The method was successfully applied to measure anthocyanidins and anthocyanins in plasma and urine samples following consumption of acute blueberry test meals.

AB - Using simple solvent extraction and enzymatic hydrolysis, a rapid LC-MS/MS method for quantification of free and conjugated forms of anthocyanidins and anthocyanins in plasma and urine samples was developed and validated. A mixed enzymatic treatment containing β-glucuronidase (100 U mL −1 ) and sulfatase (2.5 U mL −1 ) for 5 min (37 °C; pH 6) was optimal condition for deconjugation of anthocyanidins and anthocyanins in urine and plasma samples. The LC–MS/MS allowed quantifying thirteen different anthocyanidins and anthocyanins simultaneously. The developed LC–MS/MS method was precise and accurate over multiple days and nominal concentrations. The stability assessment study confirmed that the long-term storage and/or periodic use of plasma and urine samples might have a considerable impact on the stability of some anthocyanidins. The method was successfully applied to measure anthocyanidins and anthocyanins in plasma and urine samples following consumption of acute blueberry test meals.

KW - Acute blueberry consumption

KW - Anthocyanidin

KW - Anthocyanin

KW - Plasma

KW - Urine

KW - MASS-SPECTROMETRY METHOD

KW - LIQUID-CHROMATOGRAPHY

KW - HUMAN PLASMA

KW - URINE

KW - VALIDATION

KW - BIOAVAILABILITY

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DO - 10.1016/j.foodchem.2019.03.109

M3 - Article

C2 - 31000056

AN - SCOPUS:85063507980

SN - 0308-8146

VL - 290

SP - 56

EP - 63

JO - Food Chemistry

JF - Food Chemistry

ER -

Rapid method for quantification of anthocyanidins and anthocyanins in human biological samples

Abstract

Bibliographical note

Keywords

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Wendy Russell

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Rapid method for quantification of anthocyanidins and anthocyanins in human biological samples

Abstract

Bibliographical note

Keywords

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Wendy Russell

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