Regenerated soleus muscle shows reduced creatine kinase efflux after contractile activity in vitro

Juozas Baltusnikas; Audrius Kilikevicius; Tomas Venckunas; Andrej Fokin; Arimantas Lionikas; Aivaras Ratkevicius

doi:10.1139/apnm-2014-0274

Regenerated soleus muscle shows reduced creatine kinase efflux after contractile activity in vitro

Juozas Baltusnikas, Audrius Kilikevicius, Tomas Venckunas, Andrej Fokin, Arimantas Lionikas, Aivaras Ratkevicius

Medical Sciences

Lithuanian Sports University

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

9 Downloads (Pure)

Abstract

Regenerated skeletal muscles show less muscle damage after strenuous muscle exercise. The aim of the studies was to investigate if the regeneration is associated with reduced muscle creatine kinase (CK) efflux immediately after the exercise. Cryolesion was applied to the soleus muscle of 3-month-old C57BL/6J male mice. Then total CK efflux was assessed in vitro in the regenerated muscles without exercise or after 100 eccentric contractions. The same measurements were performed in the control muscles, which were not exposed to cryolesion. Regenerated muscles generated weaker (P < 0.05) twitches, but stronger (P < 0.05) 150-Hz and 300-Hz tetani with prolonged (P < 0.01) contraction times compared with the control muscles. There was no difference between regenerated and control muscles in the total CK efflux without exercise, but only control muscles showed an increase (P < 0.001) in the CK efflux after the exercise. Our results suggest that muscle regeneration is associated with modulation of contractile properties and improvement in muscle resistance to damage after eccentric exercise.

Original language	English
Pages (from-to)	129-133
Number of pages	5
Journal	Applied Physiology Nutrition and Metabolism / Physiologie Appliquée, Nutrition et Métabolisme
Volume	40
Issue number	2
Early online date	15 Oct 2014
DOIs	https://doi.org/10.1139/apnm-2014-0274
Publication status	Published - Feb 2015

Bibliographical note

Date of Acceptance: 07/10/2014

Acknowledgements
We are very grateful to Petras Jeneckas and Audrius Capskas for technical assistance. The study was supported by a grant (No. MIP-067/2012) from the Research Council of Lithuania.

Keywords

cryolesion
primary damage
muscle damage
repeated bout effect
eccentric exercise
lengthening contractions
mice

Access to Document

10.1139/apnm-2014-0274Licence: Unspecified

Baltusnikas_APNM_0274.R1
This is the author's final draft of a manuscript accepted for publication in Applied Physiology, Nutrition, and Metabolism
Accepted author manuscript, 415 KB

Cite this

Regenerated soleus muscle shows reduced creatine kinase efflux after contractile activity in vitro. / Baltusnikas, Juozas; Kilikevicius, Audrius; Venckunas, Tomas et al.
In: Applied Physiology Nutrition and Metabolism / Physiologie Appliquée, Nutrition et Métabolisme, Vol. 40, No. 2, 02.2015, p. 129-133.

Research output: Contribution to journal › Article › peer-review

@article{f2f0ae4684b94115ad9e8df6e400f530,

title = "Regenerated soleus muscle shows reduced creatine kinase efflux after contractile activity in vitro",

abstract = "Regenerated skeletal muscles show less muscle damage after strenuous muscle exercise. The aim of the studies was to investigate if the regeneration is associated with reduced muscle creatine kinase (CK) efflux immediately after the exercise. Cryolesion was applied to the soleus muscle of 3-month-old C57BL/6J male mice. Then total CK efflux was assessed in vitro in the regenerated muscles without exercise or after 100 eccentric contractions. The same measurements were performed in the control muscles, which were not exposed to cryolesion. Regenerated muscles generated weaker (P < 0.05) twitches, but stronger (P < 0.05) 150-Hz and 300-Hz tetani with prolonged (P < 0.01) contraction times compared with the control muscles. There was no difference between regenerated and control muscles in the total CK efflux without exercise, but only control muscles showed an increase (P < 0.001) in the CK efflux after the exercise. Our results suggest that muscle regeneration is associated with modulation of contractile properties and improvement in muscle resistance to damage after eccentric exercise.",

keywords = "cryolesion, primary damage, muscle damage, repeated bout effect, eccentric exercise, lengthening contractions, mice",

author = "Juozas Baltusnikas and Audrius Kilikevicius and Tomas Venckunas and Andrej Fokin and Arimantas Lionikas and Aivaras Ratkevicius",

note = "Date of Acceptance: 07/10/2014 Acknowledgements We are very grateful to Petras Jeneckas and Audrius Capskas for technical assistance. The study was supported by a grant (No. MIP-067/2012) from the Research Council of Lithuania. ",

year = "2015",

month = feb,

doi = "10.1139/apnm-2014-0274",

language = "English",

volume = "40",

pages = "129--133",

journal = "Applied Physiology Nutrition and Metabolism / Physiologie Appliqu{\'e}e, Nutrition et M{\'e}tabolisme",

issn = "1715-5312",

publisher = "National Research Council of Canada",

number = "2",

}

TY - JOUR

T1 - Regenerated soleus muscle shows reduced creatine kinase efflux after contractile activity in vitro

AU - Baltusnikas, Juozas

AU - Kilikevicius, Audrius

AU - Venckunas, Tomas

AU - Fokin, Andrej

AU - Lionikas, Arimantas

AU - Ratkevicius, Aivaras

N1 - Date of Acceptance: 07/10/2014 Acknowledgements We are very grateful to Petras Jeneckas and Audrius Capskas for technical assistance. The study was supported by a grant (No. MIP-067/2012) from the Research Council of Lithuania.

PY - 2015/2

Y1 - 2015/2

N2 - Regenerated skeletal muscles show less muscle damage after strenuous muscle exercise. The aim of the studies was to investigate if the regeneration is associated with reduced muscle creatine kinase (CK) efflux immediately after the exercise. Cryolesion was applied to the soleus muscle of 3-month-old C57BL/6J male mice. Then total CK efflux was assessed in vitro in the regenerated muscles without exercise or after 100 eccentric contractions. The same measurements were performed in the control muscles, which were not exposed to cryolesion. Regenerated muscles generated weaker (P < 0.05) twitches, but stronger (P < 0.05) 150-Hz and 300-Hz tetani with prolonged (P < 0.01) contraction times compared with the control muscles. There was no difference between regenerated and control muscles in the total CK efflux without exercise, but only control muscles showed an increase (P < 0.001) in the CK efflux after the exercise. Our results suggest that muscle regeneration is associated with modulation of contractile properties and improvement in muscle resistance to damage after eccentric exercise.

AB - Regenerated skeletal muscles show less muscle damage after strenuous muscle exercise. The aim of the studies was to investigate if the regeneration is associated with reduced muscle creatine kinase (CK) efflux immediately after the exercise. Cryolesion was applied to the soleus muscle of 3-month-old C57BL/6J male mice. Then total CK efflux was assessed in vitro in the regenerated muscles without exercise or after 100 eccentric contractions. The same measurements were performed in the control muscles, which were not exposed to cryolesion. Regenerated muscles generated weaker (P < 0.05) twitches, but stronger (P < 0.05) 150-Hz and 300-Hz tetani with prolonged (P < 0.01) contraction times compared with the control muscles. There was no difference between regenerated and control muscles in the total CK efflux without exercise, but only control muscles showed an increase (P < 0.001) in the CK efflux after the exercise. Our results suggest that muscle regeneration is associated with modulation of contractile properties and improvement in muscle resistance to damage after eccentric exercise.

KW - cryolesion

KW - primary damage

KW - muscle damage

KW - repeated bout effect

KW - eccentric exercise

KW - lengthening contractions

KW - mice

U2 - 10.1139/apnm-2014-0274

DO - 10.1139/apnm-2014-0274

M3 - Article

C2 - 25565131

SN - 1715-5312

VL - 40

SP - 129

EP - 133

JO - Applied Physiology Nutrition and Metabolism / Physiologie Appliquée, Nutrition et Métabolisme

JF - Applied Physiology Nutrition and Metabolism / Physiologie Appliquée, Nutrition et Métabolisme

IS - 2

ER -

Regenerated soleus muscle shows reduced creatine kinase efflux after contractile activity in vitro

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this