Responses in whole-body amino acid kinetics to an acute, sub-clinical endotoxin challenge in lambs

S. O. Hoskin; D. M. Bremner; G. Holtrop; G. E. Lobley

doi:10.1017/S0007114515004894

Responses in whole-body amino acid kinetics to an acute, sub-clinical endotoxin challenge in lambs

S. O. Hoskin, D. M. Bremner, G. Holtrop, G. E. Lobley^*

^*Corresponding author for this work

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Some effects of parasitism, endotoxaemia or sepsis can be mitigated by provision of extra protein. Supplemented protein may encompass a metabolic requirement for specific amino acids (AA). The current study investigates a method to identify and quantify the amounts of AA required during inflammation induced by an endotoxin challenge. One of each pair of six twin sheep was infused in the jugular vein for 20 h with either saline (control) or lipopolysaccharide (LPS, 2 ng/kg body weight per min) from Escherichia coli. Between 12 and 20 h a mixture of stable isotope-labelled AA was infused to measure irreversible loss rates. From 16 to 20 h all sheep were supplemented with a mixture of unlabelled AA infused intravenously. Blood samples were taken before the start of infusions, and then continuously over intervals between 14 and 20 h. At 20 h the sheep were euthanised, and liver and kidney samples were taken for measurement of serine-threonine dehydratase (SDH) activity. LPS infusion decreased plasma concentrations of most AA (P

Original language	English
Pages (from-to)	576-584
Number of pages	9
Journal	British Journal of Nutrition
Volume	115
Issue number	4
Early online date	14 Dec 2015
DOIs	https://doi.org/10.1017/S0007114515004894
Publication status	Published - 28 Feb 2016

Bibliographical note

Acknowledgements
The expertise of A. Graham Calder and Susan Anderson for the
various stable isotope analyses is gratefully recognised. Celine
Germaine performed the SDH measurements from the tissue
samples as part of a student placement from AgroParisTech,
Paris, France.
This study was funded as part of the core grant provided to
the Rowett Institute of Nutrition and Health, University of
Aberdeen and Biomathematics and Statistics Scotland by Rural
and Environment Science and Analytical Services, part of the
Scottish Government. Hoskin was a recipient of a FoRST (NZ)
award to study abroad.
The authors’ contributions were as follows: S. O. H. and G. E. L.
were responsible for study concept and design. S. O. H., G. E. L.
and D. M. B. were responsible for data collection and collation.
G. E. L. and G. H. were responsible for data analysis and statistical
matters. S. O. H., G. E. L. and G. H. were responsible for the
first draft and critical revision of the manuscript for important
intellectual content.
The authors declare that there are no conflicts of interest

Keywords

endotoxin lipopolysaccharide
amino acids
irreversible loss rate
serine-threonine dehydratase
sheep
acute-phase proteins
teladorsagia-circumcincta
porcine model
septic rats
metabolism
sepsis
arginine
plasma
infection

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title = "Responses in whole-body amino acid kinetics to an acute, sub-clinical endotoxin challenge in lambs",

abstract = "Some effects of parasitism, endotoxaemia or sepsis can be mitigated by provision of extra protein. Supplemented protein may encompass a metabolic requirement for specific amino acids (AA). The current study investigates a method to identify and quantify the amounts of AA required during inflammation induced by an endotoxin challenge. One of each pair of six twin sheep was infused in the jugular vein for 20 h with either saline (control) or lipopolysaccharide (LPS, 2 ng/kg body weight per min) from Escherichia coli. Between 12 and 20 h a mixture of stable isotope-labelled AA was infused to measure irreversible loss rates. From 16 to 20 h all sheep were supplemented with a mixture of unlabelled AA infused intravenously. Blood samples were taken before the start of infusions, and then continuously over intervals between 14 and 20 h. At 20 h the sheep were euthanised, and liver and kidney samples were taken for measurement of serine-threonine dehydratase (SDH) activity. LPS infusion decreased plasma concentrations of most AA (P",

keywords = "endotoxin lipopolysaccharide, amino acids, irreversible loss rate, serine-threonine dehydratase, sheep, acute-phase proteins, teladorsagia-circumcincta, porcine model, septic rats, metabolism, sepsis, arginine, plasma, infection",

author = "Hoskin, {S. O.} and Bremner, {D. M.} and G. Holtrop and Lobley, {G. E.}",

note = "Acknowledgements The expertise of A. Graham Calder and Susan Anderson for the various stable isotope analyses is gratefully recognised. Celine Germaine performed the SDH measurements from the tissue samples as part of a student placement from AgroParisTech, Paris, France. This study was funded as part of the core grant provided to the Rowett Institute of Nutrition and Health, University of Aberdeen and Biomathematics and Statistics Scotland by Rural and Environment Science and Analytical Services, part of the Scottish Government. Hoskin was a recipient of a FoRST (NZ) award to study abroad. The authors{\textquoteright} contributions were as follows: S. O. H. and G. E. L. were responsible for study concept and design. S. O. H., G. E. L. and D. M. B. were responsible for data collection and collation. G. E. L. and G. H. were responsible for data analysis and statistical matters. S. O. H., G. E. L. and G. H. were responsible for the first draft and critical revision of the manuscript for important intellectual content. The authors declare that there are no conflicts of interest",

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AU - Lobley, G. E.

N1 - Acknowledgements The expertise of A. Graham Calder and Susan Anderson for the various stable isotope analyses is gratefully recognised. Celine Germaine performed the SDH measurements from the tissue samples as part of a student placement from AgroParisTech, Paris, France. This study was funded as part of the core grant provided to the Rowett Institute of Nutrition and Health, University of Aberdeen and Biomathematics and Statistics Scotland by Rural and Environment Science and Analytical Services, part of the Scottish Government. Hoskin was a recipient of a FoRST (NZ) award to study abroad. The authors’ contributions were as follows: S. O. H. and G. E. L. were responsible for study concept and design. S. O. H., G. E. L. and D. M. B. were responsible for data collection and collation. G. E. L. and G. H. were responsible for data analysis and statistical matters. S. O. H., G. E. L. and G. H. were responsible for the first draft and critical revision of the manuscript for important intellectual content. The authors declare that there are no conflicts of interest

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KW - teladorsagia-circumcincta

KW - porcine model

KW - septic rats

KW - metabolism

KW - sepsis

KW - arginine

KW - plasma

KW - infection

U2 - 10.1017/S0007114515004894

DO - 10.1017/S0007114515004894

M3 - Article

SN - 0007-1145

VL - 115

SP - 576

EP - 584

JO - British Journal of Nutrition

JF - British Journal of Nutrition

IS - 4

ER -

Responses in whole-body amino acid kinetics to an acute, sub-clinical endotoxin challenge in lambs

Abstract

Bibliographical note

Keywords

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