Sodium-lithium countertransport, sodium-hydrogen exchange and membrane microviscosity in patients with hyperlipidaemia

M.J. Macleod, W.K. Lee, A.M. Devlin, M. Caslake, N.H. Anderson, C.J. Packard, M.H. Dominiczak, J.L. Reid, A.F. Dominiczak

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

1. We measured sodium-lithium countertransport, sodium-hydrogen exchange and membrane microviscosity in 48 individuals with familial hypercholesterolaemia, 33 subjects with hypertriglyceridaemia and 54 normolipaemic controls. Full lipid profile, blood pressure, body mass index, fasting glucose and insulin levels were also measured.

2. Subjects with hypertriglyceridaemia had higher blood pressure, body mass index, fasting glucose and insulin levels than normal controls.

3. Vmax of the sodium-lithium countertransport was elevated in the hypertriglyceridaemic group compared with controls. Across the whole group loge triacylglycerols correlated with Vmax of the sodium-lithium countertransport. There was no difference in sodium-lithium countertransport Km between the groups.

4. Sodium-hydrogen maximal proton efflux rate (Vmax) and Km were not different between the three groups. There were no correlations between sodium—hydrogen exchange and sodium—lithium countertransport parameters.

5. Microviscosity as measured by diphenylhexatriene was reduced at the core of the membrane in subjects with hypertriglyceridaemia compared with those with familial hypercholesterolaemia or normolipaemic controls, suggesting an alteration in membrane structure.

6. Changes in sodium transport in hyperlipidaemia may be mediated by changes in membrane structure resulting in altered protein conformation or turnover.
Original languageEnglish
Pages (from-to)237-246
Number of pages10
JournalClinical Science
Volume92
Issue number3
DOIs
Publication statusPublished - 1 Mar 1997
Externally publishedYes

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