Abstract
Here we provide a SOP to outline the correct procedures for performing Immunohistochemistry (IHC) to detect pathological TDP-43 in FFPE-preserved human tissue using the TDP-43 RNA Aptamer (TDP-43APT), as described in Spence and Waldron et al., 2024 published in Acta Neuropathologica (in press at the time of release of this SOP on
01/03/2024)
Users with access to Sequenza immunostaining racks and histological facilities (with fume hood) should be able to carry out all steps over two days.
This protocol uses the TDP-43APT sequence published in Zacco et al., 2022. The sequence is: CGGUGUUGCU with a 3' Biotin-TEG modification, purified using HPLC, scale: 1.0 µM synthesis.
01/03/2024)
Users with access to Sequenza immunostaining racks and histological facilities (with fume hood) should be able to carry out all steps over two days.
This protocol uses the TDP-43APT sequence published in Zacco et al., 2022. The sequence is: CGGUGUUGCU with a 3' Biotin-TEG modification, purified using HPLC, scale: 1.0 µM synthesis.
Original language | English |
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Type | Protocol: SOP |
Publisher | protocols.io |
DOIs | |
Publication status | Published - 1 Mar 2024 |