Specificity of the osmotic stress response in Candida albicans highlighted by quantitative proteomics

Mette D. Jacobsen, Robert J. Beynon, Lee A. Gethings, Amy J. Claydon, James I. Langridge, Johannes P. C. Vissers, Alistair J. P. Brown* (Corresponding Author), Dean E. Hammond* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)
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Stress adaptation is critical for the survival of microbes in dynamic environments, and in particular, for fungal pathogens to survive in and colonise host niches. Proteomic analyses have the potential to significantly enhance
our understanding of these adaptive responses by providing insight into post-transcriptional regulatory mechanisms that contribute to the outputs, as well as testing presumptions about the regulation of protein levels based on transcript profiling. Here, we used label-free, quantitative mass spectrometry to re-examine the response of the major fungal pathogen of humans, Candida albicans, to osmotic stress. Of the 1,262 proteins that were identified, 84 were down-regulated in response to 1M NaCl, reflecting the decrease in ribosome
biogenesis and translation that often accompanies stress. The 64 up-regulated proteins included central metabolic enzymes required for glycerol synthesis, a key osmolyte for this yeast, as well as proteins with functions during stress. These data reinforce the view that adaptation to salt stress involves a transient
reduction in ribosome biogenesis and translation together with the accumulation of the osmolyte, glycerol. The specificity of the response to salt stress is highlighted by the small proportion of quantified C. albicans proteins (5%) whose relative elevated abundances were statistically significant.
Original languageEnglish
Article number14492
JournalScientific Reports
Publication statusPublished - 27 Sept 2018

Bibliographical note

We are grateful to the BBSRC for funding the CRISP Consortium (Combinatorial Responses in Stress Pathways) under the SABR Initiative (Systems Approaches to Biological Research) (BB/F00513X/1; BB/F005210/1). AJPB was also funded by the BBSRC (BB/K017365/1), the ERC (C-2009-AdG-249793), the Wellcome Trust (097377), the MRC (MR/M026663/1), and the MRC Centre for Medical Mycology and the University of Aberdeen (MR/M026663/1).


  • mechanisms of disease
  • pathogens


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