Spike-Driven Glutamate Electrodiffusion Triggers Synaptic Potentiation via a Homer-Dependent mGluR-NMDAR Link

Sergiy Sylantyev; Leonid P. Savtchenko; Yaroslav Ermolyuk; Piotr Michaluk; Dmitri A. Rusakov

doi:10.1016/j.neuron.2012.11.026

Spike-Driven Glutamate Electrodiffusion Triggers Synaptic Potentiation via a Homer-Dependent mGluR-NMDAR Link

Sergiy Sylantyev, Leonid P. Savtchenko, Yaroslav Ermolyuk, Piotr Michaluk, Dmitri A. Rusakov^*

^*Corresponding author for this work

The Rowett Institute of Nutrition and Health

University College London

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

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Abstract

Electric fields of synaptic currents can influence diffusion of charged neurotransmitters, such as glutamate, in the synaptic cleft. However, this phenomenon has hitherto been detected only through sustained depolarization of large principal neurons, and its adaptive significance remains unknown. Here, we find that in cerebellar synapses formed on electrically compact granule cells, a single postsynaptic action potential can retard escape of glutamate released into the cleft. This retardation boosts activation of perisynaptic group I metabotropic glutamate receptors (mGluRs), which in turn rapidly facilitates local NMDA receptor currents. The underlying mechanism relies on a Homer-containing protein scaffold, but not GPCR- or Ca2+-dependent signaling. Through the mGluR-NMDAR interaction, the coincidence between a postsynaptic spike and glutamate release triggers a lasting enhancement of synaptic transmission that alters the basic integrate-and-spike rule in the circuitry. Our results thus reveal an electrodiffusion-driven synaptic memory mechanism that requires high-precision coincidence detection suitable for high-fidelity circuitries.

Original language	English
Pages (from-to)	528-541
Number of pages	14
Journal	Neuron
Volume	77
Issue number	3
DOIs	https://doi.org/10.1016/j.neuron.2012.11.026
Publication status	Published - 6 Feb 2013

Bibliographical note

ACKNOWLEDGMENTS
This work was supported by the Wellcome Trust, MRC, BBSRC, and ERC Advanced Grant (D.A.R.) and the Foundation for Polish Science (P.M.). The authors thank Angus Silver, Dimitri Kullmann, Kirill Volynski, and the anonymous referees for their valuable comments and Mykola Stryebkov and Ksenia Redunova for help with data processing software.

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Sylantyev_et_al_Neuron_SpikeDrivenGlutamate_VoR
Open access under CC BY license. https://creativecommons.org/licenses/by/3.0/
Final published version, 2.06 MBLicence: CC BY

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title = "Spike-Driven Glutamate Electrodiffusion Triggers Synaptic Potentiation via a Homer-Dependent mGluR-NMDAR Link",

abstract = "Electric fields of synaptic currents can influence diffusion of charged neurotransmitters, such as glutamate, in the synaptic cleft. However, this phenomenon has hitherto been detected only through sustained depolarization of large principal neurons, and its adaptive significance remains unknown. Here, we find that in cerebellar synapses formed on electrically compact granule cells, a single postsynaptic action potential can retard escape of glutamate released into the cleft. This retardation boosts activation of perisynaptic group I metabotropic glutamate receptors (mGluRs), which in turn rapidly facilitates local NMDA receptor currents. The underlying mechanism relies on a Homer-containing protein scaffold, but not GPCR- or Ca2+-dependent signaling. Through the mGluR-NMDAR interaction, the coincidence between a postsynaptic spike and glutamate release triggers a lasting enhancement of synaptic transmission that alters the basic integrate-and-spike rule in the circuitry. Our results thus reveal an electrodiffusion-driven synaptic memory mechanism that requires high-precision coincidence detection suitable for high-fidelity circuitries.",

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AU - Rusakov, Dmitri A.

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N2 - Electric fields of synaptic currents can influence diffusion of charged neurotransmitters, such as glutamate, in the synaptic cleft. However, this phenomenon has hitherto been detected only through sustained depolarization of large principal neurons, and its adaptive significance remains unknown. Here, we find that in cerebellar synapses formed on electrically compact granule cells, a single postsynaptic action potential can retard escape of glutamate released into the cleft. This retardation boosts activation of perisynaptic group I metabotropic glutamate receptors (mGluRs), which in turn rapidly facilitates local NMDA receptor currents. The underlying mechanism relies on a Homer-containing protein scaffold, but not GPCR- or Ca2+-dependent signaling. Through the mGluR-NMDAR interaction, the coincidence between a postsynaptic spike and glutamate release triggers a lasting enhancement of synaptic transmission that alters the basic integrate-and-spike rule in the circuitry. Our results thus reveal an electrodiffusion-driven synaptic memory mechanism that requires high-precision coincidence detection suitable for high-fidelity circuitries.

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Spike-Driven Glutamate Electrodiffusion Triggers Synaptic Potentiation via a Homer-Dependent mGluR-NMDAR Link

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