Technical note: methods for detecting liquid enzyme additives added to animal feeds

R. J. Wallace, G. F. Hartnell

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4 Citations (Scopus)


Methods for detecting and measuring the quantity of fibrolytic enzyme preparations added to feeds were investigated by enzymatic and tracer methods. Enzyme preparations added to corn silage, ryegrass silage, and a total mixed ration containing both silages and a concentrate could not be detected using their enzymatic activities. Glycosidase activities of solubles washed from the feed were more than an order of magnitude greater than glycosidases in the added enzymes. Carboxymethylcellulase and xylanase activity determinations, using reducing sugar release as the measurement, were subject to interference from reducing sugars present in the feed. A fluorescent tracer method, using fluorescein added at a rate of 1 g/L of feed enzymes, or 2 g/t of feed, was developed that enabled sensitive detection of liquid enzyme additions to feeds
Original languageEnglish
Pages (from-to)2731-2735
Number of pages5
JournalJournal of Animal Science
Issue number10
Publication statusPublished - Oct 2001

Bibliographical note

UI - 21578463 LA - eng RN - 0 (Contrast Media) RN - 0 (Food Additives) RN - 2321-07-5 (Fluorescein) RN - EC 3.2.1. (Glycoside Hydrolases) RN - EC 3.2.1.- (Xylosidases) RN - EC 3.2.1.- (carboxymethylcellulase) RN - EC (endo-1,3-beta-xylanase) PT - Journal Article DA - 20011127 IS - 0021-8812 SB - IM CY - United States


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