Abstract
We characterized the intravenous (i.v.) challenge model for disseminated Candida albicans infection in female BALB/c and DBA/2 mice. Clearance of fungi from the bloodstream and appearance of fungi in tissues were measured at intervals after challenge with various doses of C. albicans. The wild-type isolate SC5314 and derived strains CAF2-1 and CAI-4 transformed with CIp10 were of equal virulence in the model. Variability in mouse survival times, kidney fungal burdens and cachexia was lowest when challenge inocula were within the range 10(4)-10(5) CFU g(-1) body weight in BALB/c mice, but brain fungal burdens and outcomes in DBA/2 mice were variable for all inocula tested. Critical times in the development of infections in optimally challenged BALB/c mice were at 5-10 h (bloodstream fully cleared of fungi), 24 h (start of exponential fungal growth in kidneys) and 48 h (50% of blood cultures become positive). Differential involvement of right and left kidneys occurred almost exclusively in mice challenged with
Original language | English |
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Pages (from-to) | 151-61 |
Number of pages | 11 |
Journal | Mycoses |
Volume | 48 |
Issue number | 3 |
Early online date | 13 Apr 2005 |
DOIs | |
Publication status | Published - 1 May 2005 |
Keywords
- Animals
- Brain
- Cachexia
- Candida albicans
- Candidiasis
- Disease Models, Animal
- Female
- Fungemia
- Kidney
- Mice
- Mice, Inbred BALB C
- Mice, Inbred DBA
- Survival Analysis
- Time Factors
- Candida albicans
- mouse model
- virulence