The association of paraoxonase 1 activities, serum mRNA expression and polymorphisms with in-stent coronary restenosis; a case-control study

Sara Saffar Soflaei; Mojtaba Baktashian; Maryam Saberi-Karimian; Habibollah Esmaily; Mohsen Moohebati; Mahmoud Ebrahimi; Aida Gholoobi; Seyed Mohammad Hashemi; Hamideh Ghazizadeh; Gordon A. Ferns; Mansoor Salehi; Alireza Pasdar; Majid Ghayour-Mobarhan

doi:10.1016/j.genrep.2023.101773

The association of paraoxonase 1 activities, serum mRNA expression and polymorphisms with in-stent coronary restenosis; a case-control study

Sara Saffar Soflaei, Mojtaba Baktashian, Maryam Saberi-Karimian, Habibollah Esmaily, Mohsen Moohebati, Mahmoud Ebrahimi, Aida Gholoobi, Seyed Mohammad Hashemi, Hamideh Ghazizadeh, Gordon A. Ferns, Mansoor Salehi^*, Alireza Pasdar^*, Majid Ghayour-Mobarhan^*

^*Corresponding author for this work

Applied Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Background: PON1 polymorphisms and enzyme activity have been reported to be associated with cardiovascular disease (CVD). The aim of current study was to assess the association of these PON1 SNPs and serum enzyme activity and with restenosis following coronary artery stenting. Methods: A total of 306 unrelated Iranian patients with history of coronary stent implantation who underwent elective re-angiography were enrolled in the current study. Three single nucleotide polymorphisms (SNPs) of PON1 gene were genotyped. We used a double ARMS PCR method for genotyping at the Q192R and L55M loci, and PCR-RFLP method for C-108 T. Serum mRNA expression of PON1 was determined using SYBER green method and Roche LightCycler. Results: There were 104 patients considered as in-stent restenosis (ISR) (mean age: 60.55 ± 8.60 years), and 202 individuals as non ISR (NISR) (mean age: 61.94 ± 9.18 years). There were no significant difference between paraoxonase and arylestrase actvities of paraoxonase 1 enzyme. Furthermore, there were no significant differences in the distribution of genotypes of PON1 polymorphism with ISR compared to the control group. The AAC haplotype was the most frequent haplotype in our population study, and haplotypes were not associated with ISR occurrence. The difference between serum mRNA expression of PON1 was not significant between ISR and NISR groups. Conclusions: According to our finding it seems that paraoxonase 1 activities, the most important polymorphisms of PON1 including Q192R, L55M and C-108 T and serum mRNA expression of PON1 are not beneficial markers for ISR diagnosis.

Original language	English
Article number	101773
Journal	Gene Reports
Volume	31
Early online date	19 Apr 2023
DOIs	https://doi.org/10.1016/j.genrep.2023.101773
Publication status	Published - Jun 2023

Bibliographical note

Funding Information:
This work was supported by Mashhad University of Medical Science (MUMS) and Medical Univeristy of Isfahan (MUI), Iran.

Data Availability Statement

Data sharing is applicable under the permission of Mashhad University of Medical Sciences.

Keywords

C-108 T
In-stent restenosis
L55M
Paraoxonase 1 activity
PON1
Q192R
Serum mRNA expression

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.genrep.2023.101773

Cite this

Soflaei, S. S., Baktashian, M., Saberi-Karimian, M., Esmaily, H., Moohebati, M., Ebrahimi, M., Gholoobi, A., Hashemi, S. M., Ghazizadeh, H., Ferns, G. A., Salehi, M., Pasdar, A., & Ghayour-Mobarhan, M. (2023). The association of paraoxonase 1 activities, serum mRNA expression and polymorphisms with in-stent coronary restenosis; a case-control study. Gene Reports, 31, Article 101773. https://doi.org/10.1016/j.genrep.2023.101773

Soflaei, SS, Baktashian, M, Saberi-Karimian, M, Esmaily, H, Moohebati, M, Ebrahimi, M, Gholoobi, A, Hashemi, SM, Ghazizadeh, H, Ferns, GA, Salehi, M, Pasdar, A & Ghayour-Mobarhan, M 2023, 'The association of paraoxonase 1 activities, serum mRNA expression and polymorphisms with in-stent coronary restenosis; a case-control study', Gene Reports, vol. 31, 101773. https://doi.org/10.1016/j.genrep.2023.101773

@article{8683a8a5947f43eb9036390c9d12ad9e,

title = "The association of paraoxonase 1 activities, serum mRNA expression and polymorphisms with in-stent coronary restenosis; a case-control study",

abstract = "Background: PON1 polymorphisms and enzyme activity have been reported to be associated with cardiovascular disease (CVD). The aim of current study was to assess the association of these PON1 SNPs and serum enzyme activity and with restenosis following coronary artery stenting. Methods: A total of 306 unrelated Iranian patients with history of coronary stent implantation who underwent elective re-angiography were enrolled in the current study. Three single nucleotide polymorphisms (SNPs) of PON1 gene were genotyped. We used a double ARMS PCR method for genotyping at the Q192R and L55M loci, and PCR-RFLP method for C-108 T. Serum mRNA expression of PON1 was determined using SYBER green method and Roche LightCycler. Results: There were 104 patients considered as in-stent restenosis (ISR) (mean age: 60.55 ± 8.60 years), and 202 individuals as non ISR (NISR) (mean age: 61.94 ± 9.18 years). There were no significant difference between paraoxonase and arylestrase actvities of paraoxonase 1 enzyme. Furthermore, there were no significant differences in the distribution of genotypes of PON1 polymorphism with ISR compared to the control group. The AAC haplotype was the most frequent haplotype in our population study, and haplotypes were not associated with ISR occurrence. The difference between serum mRNA expression of PON1 was not significant between ISR and NISR groups. Conclusions: According to our finding it seems that paraoxonase 1 activities, the most important polymorphisms of PON1 including Q192R, L55M and C-108 T and serum mRNA expression of PON1 are not beneficial markers for ISR diagnosis.",

keywords = "C-108 T, In-stent restenosis, L55M, Paraoxonase 1 activity, PON1, Q192R, Serum mRNA expression",

author = "Soflaei, {Sara Saffar} and Mojtaba Baktashian and Maryam Saberi-Karimian and Habibollah Esmaily and Mohsen Moohebati and Mahmoud Ebrahimi and Aida Gholoobi and Hashemi, {Seyed Mohammad} and Hamideh Ghazizadeh and Ferns, {Gordon A.} and Mansoor Salehi and Alireza Pasdar and Majid Ghayour-Mobarhan",

note = "Funding Information: This work was supported by Mashhad University of Medical Science (MUMS) and Medical Univeristy of Isfahan (MUI), Iran. ",

year = "2023",

month = jun,

doi = "10.1016/j.genrep.2023.101773",

language = "English",

volume = "31",

journal = "Gene Reports",

issn = "2452-0144",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - The association of paraoxonase 1 activities, serum mRNA expression and polymorphisms with in-stent coronary restenosis; a case-control study

AU - Soflaei, Sara Saffar

AU - Baktashian, Mojtaba

AU - Saberi-Karimian, Maryam

AU - Esmaily, Habibollah

AU - Moohebati, Mohsen

AU - Ebrahimi, Mahmoud

AU - Gholoobi, Aida

AU - Hashemi, Seyed Mohammad

AU - Ghazizadeh, Hamideh

AU - Ferns, Gordon A.

AU - Salehi, Mansoor

AU - Pasdar, Alireza

AU - Ghayour-Mobarhan, Majid

N1 - Funding Information: This work was supported by Mashhad University of Medical Science (MUMS) and Medical Univeristy of Isfahan (MUI), Iran.

PY - 2023/6

Y1 - 2023/6

N2 - Background: PON1 polymorphisms and enzyme activity have been reported to be associated with cardiovascular disease (CVD). The aim of current study was to assess the association of these PON1 SNPs and serum enzyme activity and with restenosis following coronary artery stenting. Methods: A total of 306 unrelated Iranian patients with history of coronary stent implantation who underwent elective re-angiography were enrolled in the current study. Three single nucleotide polymorphisms (SNPs) of PON1 gene were genotyped. We used a double ARMS PCR method for genotyping at the Q192R and L55M loci, and PCR-RFLP method for C-108 T. Serum mRNA expression of PON1 was determined using SYBER green method and Roche LightCycler. Results: There were 104 patients considered as in-stent restenosis (ISR) (mean age: 60.55 ± 8.60 years), and 202 individuals as non ISR (NISR) (mean age: 61.94 ± 9.18 years). There were no significant difference between paraoxonase and arylestrase actvities of paraoxonase 1 enzyme. Furthermore, there were no significant differences in the distribution of genotypes of PON1 polymorphism with ISR compared to the control group. The AAC haplotype was the most frequent haplotype in our population study, and haplotypes were not associated with ISR occurrence. The difference between serum mRNA expression of PON1 was not significant between ISR and NISR groups. Conclusions: According to our finding it seems that paraoxonase 1 activities, the most important polymorphisms of PON1 including Q192R, L55M and C-108 T and serum mRNA expression of PON1 are not beneficial markers for ISR diagnosis.

AB - Background: PON1 polymorphisms and enzyme activity have been reported to be associated with cardiovascular disease (CVD). The aim of current study was to assess the association of these PON1 SNPs and serum enzyme activity and with restenosis following coronary artery stenting. Methods: A total of 306 unrelated Iranian patients with history of coronary stent implantation who underwent elective re-angiography were enrolled in the current study. Three single nucleotide polymorphisms (SNPs) of PON1 gene were genotyped. We used a double ARMS PCR method for genotyping at the Q192R and L55M loci, and PCR-RFLP method for C-108 T. Serum mRNA expression of PON1 was determined using SYBER green method and Roche LightCycler. Results: There were 104 patients considered as in-stent restenosis (ISR) (mean age: 60.55 ± 8.60 years), and 202 individuals as non ISR (NISR) (mean age: 61.94 ± 9.18 years). There were no significant difference between paraoxonase and arylestrase actvities of paraoxonase 1 enzyme. Furthermore, there were no significant differences in the distribution of genotypes of PON1 polymorphism with ISR compared to the control group. The AAC haplotype was the most frequent haplotype in our population study, and haplotypes were not associated with ISR occurrence. The difference between serum mRNA expression of PON1 was not significant between ISR and NISR groups. Conclusions: According to our finding it seems that paraoxonase 1 activities, the most important polymorphisms of PON1 including Q192R, L55M and C-108 T and serum mRNA expression of PON1 are not beneficial markers for ISR diagnosis.

KW - C-108 T

KW - In-stent restenosis

KW - L55M

KW - Paraoxonase 1 activity

KW - PON1

KW - Q192R

KW - Serum mRNA expression

UR - http://www.scopus.com/inward/record.url?scp=85152737833&partnerID=8YFLogxK

U2 - 10.1016/j.genrep.2023.101773

DO - 10.1016/j.genrep.2023.101773

M3 - Article

AN - SCOPUS:85152737833

SN - 2452-0144

VL - 31

JO - Gene Reports

JF - Gene Reports

M1 - 101773

ER -

The association of paraoxonase 1 activities, serum mRNA expression and polymorphisms with in-stent coronary restenosis; a case-control study

Abstract

Bibliographical note

Data Availability Statement

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this