Cardiovascular diseases (CVD) are the major cause of morbidity and mortality worldwide. The consumption of healthy diets rich in polyphenols has been inversely associated with the development of CVD. This study evaluated the effects of green coffee bean (GCBE) and yerba mate (YMPE) phenolic extracts, the main phenolic and methylxanthines constituents (5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, caffeine, and theobromine), and their main metabolites (caffeic acid, ferulic acid, dihydrocaffeic acid -DHCA- and dihydroferulic acid -DHFA-) on platelet activation in vitro. Upon incubation with different doses (0.01 – 100 µg/mL or µM) of each compound, adenosine 5’-diphosphate-induced P-selectin expression and fibrinogen binding were determined using whole blood flow cytometry. Platelet P-selectin expression was significantly decreased by YMPE and all phenolic and methylxanthines constituents at physiological concentrations, compared with control, whereas fibrinogen binding on platelets was significantly increased. The colonic metabolites (DHCA and DHFA) had stronger inhibitory effects on P-selectin expression than their phenolic precursors, suggesting an increase in the efficacy to modulate platelet activation with the metabolism of the phenolic compounds.
Bibliographical noteThis work was funded by the Spanish Ministry of Economy and Competitivity (projects AGL2010- 18269 and AGL 2015-69986-R). G.B. is a FPI fellow (BES-2011-047476) granted with a bursary for short stays from MINECO (EEBB-I-14-08802). The Rowett Institute of Nutrition and Health receives funding from the Scottish Government Rural and Environment Science and Analytical Services (RESAS). The funding bodies had no involvement in the design and execution of the study.
- green coffee
- yerba mate
- phenolic compounds
- platelet activation