Abstract
BACKGROUND: Autoimmune uveitis is a leading cause of visual impairment in developed countries in patients of working age. Animal models of experimental autoimmune uveitis (EAU) have been established to serve as a useful template for novel therapeutic approaches.
METHODS: Experimental autoimmune uveitis is induced in C57BL/6 mice by subcutaneous application of interphotoreceptor retinoid binding protein in complete Freund's adjuvant and pertussis toxin. Clinical and histological grading is used to assess the inflammation intensity of EAU.
RESULTS: The protocol of induction of EAU in mice hides several important aspects, which are crucial for developing the disease. These details have to be addressed to ensure reproducible disease induction. We describe our experience in establishing the model by pointing out the critical steps in EAU protocol which we found important.
CONCLUSION: The mouse model of EAU has practical value for preclinical studies, is robust and well established. However, the induction of inflammation of the eye can be quite challenging when important details of the protocol are not recognized and adhered to.
| Original language | English |
|---|---|
| Pages (from-to) | 140-142 |
| Number of pages | 3 |
| Journal | Biomedical Papers of the Medical Faculty of the University PalackÝ, Olomouc, Czechoslovakia |
| Volume | 160 |
| Issue number | 1 |
| Early online date | 10 Nov 2015 |
| DOIs | |
| Publication status | Published - Mar 2016 |
Bibliographical note
ACKNOWLEDGEMENTThis paper was supported by research project from the Ministry of Heath of the Czech Republic IGA MZ CR NT/14017-3/2013 and SVV UK 260148/2015.
Keywords
- experimental autoimmune uveitis
- C57BL/6 mouse strain
- mouse model
- induction protocol
- chronic posterior autoimmune uveitis